著者は、オーストラリア政府研究プログラムに奨学金研修を通じてモルガン Philp の支援を認めると思います。

1. 色テスト試薬溶液の調製
注: 乾燥の 100 mL ビーカーに銅硝酸三水和物の 0.12 g の重量を量る。純水 (DI) 30 mL を追加し、慎重にすべての固体を溶解する室温で旋回します。100 mL のメスフラスコにこの溶液を注ぐし、純水の校正マークがいっぱい。この準備されたソリューションは試薬 1 です。 注: 試薬 1 は、他の銅 (ii) 塩、例えば銅 (ii) 塩化物を用いて調製できます。
<ol><li>乾燥 100 mL ビーカーに 2, 9-ジメチル-1, 10-フェナントロリン (ネオクプロイン) 系の 0.11 グラムの重量を量る。0.10 mol/L の塩酸 (HCl) の 50 mL を追加し、室温で固体の溶解を促進するためにガラスの攪拌棒を使用します。100 mL のメスフラスコにこの溶液を注ぐし、0.10 mol/L HCl の校正マークがいっぱい。この準備されたソリューションは試薬 2 です。 注意: ネオクプロインは急性毒性は、皮膚刺激性、眼に対する重篤な損傷を引き起こす可能性が。暴露のリスクを最小限に処理中の安全メガネを着用します。 注: ネオクプロインは水に難溶性のみ、したがって、希薄な酸はこの試薬を準備し、すべての固体の溶解が確認されます。</li>
	<li>乾燥 100 mL ビーカーに酢酸ナトリウム 16.4 g の重量を量る。・ ディ ・水 50 mL を加えて、室温で固体の溶解を促進するためにガラスの攪拌棒を使用します。100 mL のメスフラスコにこの溶液を注ぐし、純水の校正マークがいっぱい。この準備されたソリューションは試薬 3 です。 注: プロトコルはここで一時停止することができます。試薬は安定性の高い、室温で 12 ヶ月間保存できます。</li>
</ol>2 色テスト
<ol><li>1 つのきれいな磁器スポット プレート、3 使い捨てピペット、手順 2.1、1 つのきれいなヘラ、電気ホット プレート、テストするサンプル/押収した材料で作製した 3 つの試薬ソリューションを収集します。</li>
	<li>小さいを配置、ヘラを使って、ピンの頭サイズの量 (約 0.1 0.2 mg) 3 つに未知の試料の分離磁器スポット プレートの井戸。4-methylmethcathinone 塩酸 (4-MMC)、合成 cathinone 参照サンプル (肯定的な制御) の同量を 3 つの隣接する井空 (空のコントロール) と別の 3 つの井戸を残します。 注: 最寄りの試験面は磁器スポット プレートです。これらが使用できない場合は、プラスチック マイクロウェル プレートまたは半マイクロ テスト チューブを使用します。</li>
	<li>使い捨てのピペットを使用して、空白および陽性対照の井戸だけでなく、サンプルも硝酸銅溶液 (試薬 1) の 5 滴を追加します。</li>
	<li>2 番目の使い捨てピペットを使用して、各サンプルに加えて、空白および陽性対照井戸にネオクプロイン ソリューション (試薬 2) 2 滴を追加します。</li>
	<li>3 使い捨てのピペットを使用して、各サンプルの空白および陽性対照井戸に加えて、よくする酢酸ナトリウム溶液 (試薬 3) 2 滴を追加します。 注: ソリューションのターン ライトブルー。</li>
	<li>電気のホット プレートの上に直接スポット プレートは 80 ° C のセット磁器を配置します。 注: は、ホット プレート上で直接プラスチック マイクロウェル プレートを加熱しないでください。プラスチック製のプレートを設定する浅い沸騰水浴を準備します。小さな沸騰水浴中半マイクロ テスト チューブを熱します。色の変化には厚さとスポット プレートの組成が異なりますを観察に必要な正確な時間。 注意: 防止処理スポット板火傷するとき注意してください。</li>
	<li>10 分間加熱後肉眼による観察し最終的な色の変化に注意してくださいまたは最終的な色変化の写真を撮る。 注: 使用より白い背景色の変更を視覚化します。</li>
</ol>

<ol>
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著者が明らかに何もありません。

この色テスト プロトコルはアル Obaidらによって公開された実験的な作品から適応されました。18作者が色の変化を示した cathinone アラビアチャノキ植物から抽出の存在下で発生します。公開されているプロトコルに対する変更が推定の違法薬物検出への応用を予見する必要でした。最も重要な考慮事項は、反応のスケールを減らすことでした。本稿で説明されたプロ?...

違法薬物市場は進化し、変化する市場に適応し続けることにより従来のビジネスと同様に動作します。現代の科学技術の進歩によって、具体的には、強力なコミュニケーションのグローバルな不拡散を見ている暗い純1と広範な知識を共有するオンライン フォーラム2を介してユーザー間で高められたオンライン購入。化学の進歩と組み合わせることで、新しい向精神物質 (NPS) の急速な出現は国際および国民の薬剤制御のための深刻な挑戦を作成されます。
NPS は、国際的な制御の下で薬剤と同様の効果を持つ虐待の危険性のある物質です。「法的」の選択肢として販売当初、739 NPS と報告された国際連合事務局薬剤および罪 (UNODC) の 2009 年と 20163間。最新のアニュアル レポートによると NPS のレコード番号は、オーストラリアとの国境に、これらの分析の多数合成 cathinones4として識別されるさらに握られました。グローバル規模で合成 cathinones の発作が着実に増加して以来、2010 年に最初に報告され、最もよく握られた NPS5の 1 つ。
NPS によってもたらされる課題は、ディスカッション6、7の主に公開されたトピックをされています。法医学研究所、法執行担当者は、検出して彼らの急速な出現中に NPS を識別する場所に適切なメソッドがなければ不利に残っていた。握られた材料の合成 cathinones を含む NPS の検出に広範な研究は、ガスクロマトグラフィー質量分析法 (GC/MS)8と液体クロマトグラフィー ・高解像度質量分析法 (LC HRMS)9を採用しています。検証的解析。赤外ラマン分光の10とリアルタイム質量分析法 (ダーツ MS)11,の直接分析などの周囲のイオン化質量分析法による分析を見ている最小限のサンプル準備のための需要の増加12. 分野で迅速、高感度の分析の必要性は法律施行13紙スプレー イオン化質量分析法 (PSI MS) の使用するためのポータブル デバイスへの取り込みを見ても。多くの楽器のテクニックは、高感度検出と定量結果検証分析を提供します。しかし、高いスループット分析のためサンプルの準備、実行時間、および計器飛行訓練とメンテナンスのため時間がかかる彼らすることができます。
推定色テストは、テスト サンプル14で特定の薬物クラスの存在の有無を示唆する設計されています。科学的なワーキング グループのため、解析の押収した薬 (SWGDRUG) は、紫外分光法と免疫15と並んで、最も目の肥えた電力手法としてテスト色を分類します。ただし、他の技術と比較して大幅に低コストで迅速な結果を提供する手段として法の執行および他の保証人員によってまだ広く用いられます。主な利点提供スポット色試験方法、ポータブル テスト キットを使用してフィールドでそれらを実行する機能です。
色テストの選択性は、実験試薬と色の変化を作成する興味の薬剤クラス間で発生する個々 の化学反応に依存します。現在推定テスト プロトコル合成 cathinones のみを検出するための特定のテストを欠如します。特異性を欠いているし、有害物質が含まれている一般的に使用される試薬が用いられます。その他の推奨される試薬ない可能な合成 cathinone 物質16の大規模な数の検査を受けました。
この作業の目的は、未知の組成の違法物質の合成 cathinones の予備審査のための利害関係者によって容易に採用することができます単純な色テスト プロトコルを提示することです。利害関係者には、法執行機関、国境保護機関、法医学研究所、その他関連するセキュリティ担当者が含まれます。提案手法では、電子を受け入れる銅複雑な試薬、電子豊富な合成 cathinone 薬物分子間で発生する酸化還元反応を採用しています。これらの化学的方法の開発を使用して、合成 cathinones の存在を示唆する推定の色テストの形でそれらを適用できます。

<table><tbody><tr><td>&lt;strong&gt;Chemicals&lt;/strong&gt;</td><td></td><td></td><td></td></tr><tr><td>&lt;strong&gt;&lt;em&gt;Reagents and solvents&lt;/em&gt;&lt;/strong&gt;</td><td></td><td></td><td></td></tr><tr><td>neocuproine hemihydrate</td><td>Sigma-Aldrich</td><td>72090</td><td>&amp;ge;99.0%. Acute toxicity</td></tr><tr><td>copper(II) nitrate trihydrate</td><td>Sigma Aldrich</td><td>61197</td><td>98.0%-103%</td></tr><tr><td>sodium acetate</td><td>Ajax Finechem</td><td>AJA680</td><td>anhydrous</td></tr><tr><td>hydrochloric acid</td><td>RCI Labscan</td><td>RP 1106</td><td>36%. Corrosive</td></tr><tr><td>&lt;strong&gt;Name&lt;/strong&gt;</td><td>&lt;strong&gt;Company&lt;/strong&gt;</td><td>&lt;strong&gt;Catalog Number&lt;/strong&gt;</td><td>&lt;strong&gt;Comments&lt;/strong&gt;</td></tr><tr><td>&lt;em&gt;&lt;strong&gt;Powders&lt;/strong&gt;&lt;/em&gt;</td><td></td><td></td><td></td></tr><tr><td>ascorbic acid</td><td>AJAX Finechem UNIVAR</td><td>104</td><td>L</td></tr><tr><td>benzocaine</td><td>Sigma-Aldrich</td><td>E1501</td><td></td></tr><tr><td>benzoic acid</td><td>Sigma-Aldrich</td><td>242381</td><td>&amp;ge;99.5%</td></tr><tr><td>boric acid</td><td>Silform Chemicals</td><td>R27410</td><td></td></tr><tr><td>caffeine</td><td>Sigma-Aldrich</td><td>C0750</td><td></td></tr><tr><td>cellulose</td><td>Sigma-Aldrich</td><td>435236</td><td>microcrystalline</td></tr><tr><td>calcium chloride</td><td>AJAX Finechem UNILAB</td><td>960</td><td></td></tr><tr><td>citric acid</td><td>AJAX Finechem UNIVAR</td><td>160</td><td></td></tr><tr><td>codeine phosphate</td><td>Glaxo</td><td>-</td><td>Acute toxicity</td></tr><tr><td>cysteine</td><td>Sigma-Aldrich</td><td>168149</td><td>L</td></tr><tr><td>dimethylsulfone</td><td>Sigma-Aldrich</td><td>M81705</td><td>98%</td></tr><tr><td>ephedrine HCl</td><td>Sigma-Aldrich</td><td>285749</td><td>99%. Acute toxicity</td></tr><tr><td>glucose</td><td>AJAX Finechem UNIVAR</td><td>783</td><td>D, anhydrous</td></tr><tr><td>glutathione</td><td>AJAX Finechem UNILAB</td><td>234</td><td></td></tr><tr><td>glycine</td><td>AJAX Finechem UNIVAR</td><td>1083</td><td></td></tr><tr><td>lactose</td><td>Sigma</td><td>L254</td><td>D, monohydrate</td></tr><tr><td>levamisole HCl</td><td>Sigma-Aldrich</td><td>PHR1798</td><td>Acute toxicity</td></tr><tr><td>magnesium sulphate</td><td>Scharlau</td><td>MA0080</td><td>anhydrous, extra pure</td></tr><tr><td>maltose</td><td>AJAX Finechem LABCHEM</td><td>1126</td><td>Bacteriological</td></tr><tr><td>mannitol</td><td>AJAX Finechem UNIVAR</td><td>310</td><td></td></tr><tr><td>O-acetylsalicylic Acid</td><td>Sigma-Aldrich</td><td>A5376</td><td></td></tr><tr><td>phenethylamine</td><td>Sigma-Aldrich</td><td>241008</td><td></td></tr><tr><td>phenolphthalein</td><td>AJAX Finechem LABCHEM</td><td>368</td><td>Acute toxicity</td></tr><tr><td>potassium carbonate</td><td>Chem-Supply</td><td>PA021</td><td>AR, anhydrous</td></tr><tr><td>sodium carbonate</td><td>Chem-Supply</td><td>SA099</td><td>AR, anhydrous</td></tr><tr><td>sodium chloride</td><td>Rowe Scientific</td><td>CC10363</td><td></td></tr><tr><td>starch</td><td>AJAX Finechem UNILAB</td><td>1254</td><td>soluble</td></tr><tr><td>stearic acid</td><td>AJAX Finechem UNILAB</td><td>1255</td><td></td></tr><tr><td>sucrose</td><td>AJAX Finechem UNIVAR</td><td>530</td><td></td></tr><tr><td>tartaric acid</td><td>AJAX Finechem UNIVAR</td><td>537</td><td>(+)</td></tr><tr><td>&lt;strong&gt;Name&lt;/strong&gt;</td><td>&lt;strong&gt;Company&lt;/strong&gt;</td><td>&lt;strong&gt;Catalog Number&lt;/strong&gt;</td><td>&lt;strong&gt;Comments&lt;/strong&gt;</td></tr><tr><td>&lt;em&gt;&lt;strong&gt;Household products&lt;/strong&gt;&lt;/em&gt;</td><td></td><td></td><td></td></tr><tr><td>artificial sweetener</td><td>ALDI Be Light</td><td>n/a</td><td>Contains aspartame</td></tr><tr><td>brown sugar</td><td>CSR</td><td>n/a</td><td></td></tr><tr><td>icing sugar</td><td>CSR</td><td>n/a</td><td></td></tr><tr><td>caster sugar</td><td>CSR</td><td>n/a</td><td></td></tr><tr><td>paracetamol tablet</td><td>Panadol</td><td>n/a</td><td></td></tr><tr><td>protein powder</td><td>Aussie Bodies ProteinFX</td><td>n/a</td><td></td></tr><tr><td>self-raising</td><td>Woolworths Australia Homebrand</td><td>n/a</td><td></td></tr><tr><td>plain flour</td><td>Woolworths Australia Homebrand</td><td>n/a</td><td></td></tr><tr><td>&lt;strong&gt;Name&lt;/strong&gt;</td><td>&lt;strong&gt;Company&lt;/strong&gt;</td><td>&lt;strong&gt;Catalog Number&lt;/strong&gt;</td><td>&lt;strong&gt;Comments&lt;/strong&gt;</td></tr><tr><td>&lt;strong&gt;Reference compounds&lt;/strong&gt;</td><td></td><td></td><td>controlled or illegal substances</td></tr><tr><td>&lt;em&gt;&lt;strong&gt;Cathinone-type substances&lt;/strong&gt;&lt;/em&gt;</td><td></td><td></td><td></td></tr><tr><td>1-(4-methoxyphenyl)-2-(1-pyrrolidinyl)-1-propanone HCl (MOPPP)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D1024</td><td>Acute toxicity potential</td></tr><tr><td>1-phenyl-2-methylamino-pentan-1-one HCl</td><td>Lipomed</td><td>PTD-1507-HC</td><td>Acute toxicity potential</td></tr><tr><td>2,3-dimethylmethcathinone HCl (2,3-DMMC)</td><td>Chiron Chemicals</td><td>10970.12</td><td>Acute toxicity potential</td></tr><tr><td>2,4,5-trimethylmethcathinone HCl (2,4,5-TMMC)</td><td>Chiron Chemicals</td><td>10927.13</td><td>Acute toxicity potential</td></tr><tr><td>2,4-dimethylmethcathinone HCl (2,4-DMMC)</td><td>Chiron Chemicals</td><td>10971.12</td><td>Acute toxicity potential</td></tr><tr><td>2-benzylamino-1-(3,4-methylenedioxyphenyl)-1-butanone HCl (BMDB)</td><td>Chiron Chemicals</td><td>10925.18</td><td>Acute toxicity potential</td></tr><tr><td>2-fluoromethcathinone HCl (2-FMC)</td><td>LGC Standards</td><td>LGCFOR 1275.64</td><td>Acute toxicity potential</td></tr><tr><td>2-methylmethcathinone HCl (2-MMC)</td><td>LGC Standards</td><td>LGCFOR 1387.02</td><td>Acute toxicity potential</td></tr><tr><td>3,4-methylenedioxy-&amp;alpha;-pyrrolidinobutiophenone (MDPBP) HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D973</td><td>Acute toxicity potential</td></tr><tr><td>3,4-dimethylmethcathinone HCl (DMMC)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D962</td><td>Acute toxicity potential</td></tr><tr><td>3,4-methylenedioxymethcathinone HCl (MDMC)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D942</td><td>Acute toxicity potential</td></tr><tr><td>3,4-methylenedioxy-N,N-dimethylcathinone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D977</td><td>Acute toxicity potential</td></tr><tr><td>3,4-methylenedioxypyrovalerone HCl (MDPV)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D951b</td><td>Acute toxicity potential</td></tr><tr><td>3-bromomethcathinone HCl (3-BMC)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D1035</td><td>Acute toxicity potential</td></tr><tr><td>3-fluoromethcathinone HCl (3-FMC)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D947b</td><td>Acute toxicity potential</td></tr><tr><td>3-methylmethcathinone HCl (3-MMC)</td><td>LGC Standards</td><td>LGCFOR 1387.03</td><td>Acute toxicity potential</td></tr><tr><td>4-bromomethcathinone HCl (4-BMC)</td><td>LGC Standards</td><td>LGCFOR 1387.11</td><td>Acute toxicity potential</td></tr><tr><td>4-fluoromethcathinone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D969</td><td>Acute toxicity potential</td></tr><tr><td>4-methoxymethcathinone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D952</td><td>Acute toxicity potential</td></tr><tr><td>4-methylethylcathinone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D968</td><td>Acute toxicity potential</td></tr><tr><td>4-methylmethcathinone HCl (4-MMC)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D937b</td><td>Acute toxicity potential</td></tr><tr><td>4-methyl-N-benzylcathinone&amp;nbsp;HCl (4-MBC)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D1026</td><td>Acute toxicity potential</td></tr><tr><td>4-methyl-pyrrolidinopropiophenone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D964</td><td>Acute toxicity potential</td></tr><tr><td>4-methyl-&amp;alpha;-pyrrolidinobutiophenone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D974</td><td>Acute toxicity potential</td></tr><tr><td>cathinone HCl (bk-amphetamine)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D929</td><td>Acute toxicity potential</td></tr><tr><td>dibutylone HCl (bk-DMBDB)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D1027</td><td>Acute toxicity potential</td></tr><tr><td>iso-ethcathinone HCl</td><td>Chiron Chemicals</td><td>10922.11</td><td>Acute toxicity potential</td></tr><tr><td>methcathinone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D724</td><td>Acute toxicity potential</td></tr><tr><td>methylenedioxy-&amp;alpha;-pyrrolidinopropiophenone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D960</td><td>Acute toxicity potential</td></tr><tr><td>N,N-diethylcathinone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D957</td><td>Acute toxicity potential</td></tr><tr><td>N,N-dimethylcathinone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D958</td><td>Acute toxicity potential</td></tr><tr><td>naphthylpyrovalerone HCl (naphyrone)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D981</td><td>Acute toxicity potential</td></tr><tr><td>N-ethyl-3,4-methylenedioxycathinone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D959</td><td>Acute toxicity potential</td></tr><tr><td>N-ethylbuphedrone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D1013</td><td>Acute toxicity potential</td></tr><tr><td>N-ethylcathinone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D938b</td><td>Acute toxicity potential</td></tr><tr><td>pentylone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D992</td><td>Acute toxicity potential</td></tr><tr><td>pyrovalerone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D985</td><td>Acute toxicity potential</td></tr><tr><td>&amp;alpha;-dimethylaminobutyrophenone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D1011</td><td>Acute toxicity potential</td></tr><tr><td>&amp;alpha;-dimethylaminopentiophenone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D1006</td><td>Acute toxicity potential</td></tr><tr><td>&amp;alpha;-ethylaminopentiophenone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D1005</td><td>Acute toxicity potential</td></tr><tr><td>&amp;alpha;-pyrrolidinobutiophenone HCl (&amp;alpha;-PBP)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D1012</td><td>Acute toxicity potential</td></tr><tr><td>&amp;alpha;-pyrrolidinopentiophenone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D986b</td><td>Acute toxicity potential</td></tr><tr><td>&amp;alpha;-pyrrolidinopropiophenone HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D956</td><td>Acute toxicity potential</td></tr><tr><td>&amp;beta;-keto-N-methyl-3,4-benzodioxyolylbutanamine HCl (bk-MBDB)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D948</td><td>Acute toxicity potential</td></tr><tr><td>&lt;strong&gt;Name&lt;/strong&gt;</td><td>&lt;strong&gt;Company&lt;/strong&gt;</td><td>&lt;strong&gt;Catalog Number&lt;/strong&gt;</td><td>&lt;strong&gt;Comments&lt;/strong&gt;</td></tr><tr><td>&lt;em&gt;&lt;strong&gt;Other substances&lt;/strong&gt;&lt;/em&gt;</td><td></td><td></td><td></td></tr><tr><td>(-)-ephedrine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>M924</td><td>Acute toxicity potential</td></tr><tr><td>(-)-methylephedrine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>M243</td><td>Acute toxicity potential</td></tr><tr><td>(+)-cathine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>M297</td><td>Acute toxicity potential</td></tr><tr><td>(+/-)- 3,4-methylenedioxyamphetamine HCl (MDA)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D842</td><td>Acute toxicity potential</td></tr><tr><td>(+/-)- N-methyl-3,4-methylenedioxyamphetamine HCl (MDMA)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D792c</td><td>Acute toxicity potential</td></tr><tr><td>(+/-)-methamphetamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D816e</td><td>Acute toxicity potential</td></tr><tr><td>(+/-)-N-ethyl-3,4-methylenedioxyamphetamine HCl (MDEA)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D739c</td><td>Acute toxicity potential</td></tr><tr><td>(+/-)-N-methyl-1-(3,4-methylenedioxyphenyl)-2-butylamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D450a</td><td>Acute toxicity potential</td></tr><tr><td>(+/-)-phenylpropanolamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>M296</td><td>Acute toxicity potential</td></tr><tr><td>(2S*,3R*)-2-methyl-3-[3,4-(methylenedioxy)phenyl]glycidic acid methyl ester</td><td>Australian Government National Measurement Institute (NMI)</td><td>D903</td><td>Acute toxicity potential</td></tr><tr><td>1-(3-chlorophenyl)piperazine HCl (mCPP)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D907</td><td>Acute toxicity potential</td></tr><tr><td>1-[3-(trifluoromethyl)phenyl]piperazine HCl (TFMPP)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D906</td><td>Acute toxicity potential</td></tr><tr><td>1-benzylpiperazine HCl (BZP)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D905</td><td>Acute toxicity potential</td></tr><tr><td>2,5-dimethoxy-4-iodophenylethylamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D922</td><td>Acute toxicity potential</td></tr><tr><td>2,5-dimethoxy-4-methylamphetamine HCl (DOM)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D470b</td><td>Acute toxicity potential</td></tr><tr><td>2,5-dimethoxy-4-propylthio-phenylethylamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D919</td><td>Acute toxicity potential</td></tr><tr><td>2,5-dimethoxyamphetamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D749</td><td>Acute toxicity potential</td></tr><tr><td>2-bromo-4-methylpropiophenone</td><td>Synthesised in-house</td><td>n/a</td><td>Acute toxicity potential</td></tr><tr><td>2-fluoroamphetamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D946</td><td>Acute toxicity potential</td></tr><tr><td>2-fluoromethamphetamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D933</td><td>Acute toxicity potential</td></tr><tr><td>3,4-dimethoxyamphetamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D453b</td><td>Acute toxicity potential</td></tr><tr><td>3,4-methylenedioxyphenyl-2-propanone (MDP2P)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D810b</td><td>Acute toxicity potential</td></tr><tr><td>4-bromo-2,5-dimethoxyamphetamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D396b</td><td>Acute toxicity potential</td></tr><tr><td>4-bromo-2,5-dimethoxyphenethylamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D758b</td><td>Acute toxicity potential</td></tr><tr><td>4-fluoroamphetamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D943b</td><td>Acute toxicity potential</td></tr><tr><td>4-fluorococaine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D854b</td><td>Acute toxicity potential</td></tr><tr><td>4-fluoromethamphetamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D934</td><td>Acute toxicity potential</td></tr><tr><td>4-hydroxyamphetamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D824b</td><td>Acute toxicity potential</td></tr><tr><td>4-methoxyamphetamine HCl (PMA)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D756</td><td>Acute toxicity potential</td></tr><tr><td>4-methoxymethamphetamine HCl (PMMA)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D908b</td><td>Acute toxicity potential</td></tr><tr><td>4-methylmethamphetamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D963</td><td>Acute toxicity potential</td></tr><tr><td>4-methylpropiophenone</td><td>Sigma-Aldrich</td><td>517925</td><td>Acute toxicity potential</td></tr><tr><td>5-methoxy-N,N-diallyltryptamine</td><td>Australian Government National Measurement Institute (NMI)</td><td>D954</td><td>Acute toxicity potential</td></tr><tr><td>amphetamine sulphate</td><td>Australian Government National Measurement Institute (NMI)</td><td>D420d</td><td>Acute toxicity potential</td></tr><tr><td>cocaine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D747b</td><td>Acute toxicity potential</td></tr><tr><td>dimethamphetamine (DMA)</td><td>Australian Government National Measurement Institute (NMI)</td><td>D693d</td><td>Acute toxicity potential</td></tr><tr><td>gamma-hydroxy butyrate</td><td>Australian Government National Measurement Institute (NMI)</td><td>D812b</td><td>Acute toxicity potential</td></tr><tr><td>heroin HCl</td><td>LGC Standards</td><td>LGCFOR 0037.20</td><td>Acute toxicity potential</td></tr><tr><td>ketamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D686b</td><td>Acute toxicity potential</td></tr><tr><td>methoxetamine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D989</td><td>Acute toxicity potential</td></tr><tr><td>methylamine HCl</td><td>Sigma-Aldrich</td><td>M0505</td><td>Acute toxicity potential</td></tr><tr><td>phencyclidine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D748</td><td>Acute toxicity potential</td></tr><tr><td>phentermine HCl</td><td>Australian Government National Measurement Institute (NMI)</td><td>D781</td><td>Acute toxicity potential</td></tr><tr><td>triethylamine</td><td>Sigma-Aldrich</td><td>T0886</td><td>Acute toxicity, corrosive, flammable</td></tr><tr><td>&lt;strong&gt;Name&lt;/strong&gt;</td><td>&lt;strong&gt;Company&lt;/strong&gt;</td><td>&lt;strong&gt;Catalog Number&lt;/strong&gt;</td><td>&lt;strong&gt;Comments&lt;/strong&gt;</td></tr><tr><td>&lt;strong&gt;Equipment&lt;/strong&gt;</td><td></td><td></td><td></td></tr><tr><td>12-well porcelain spot plates</td><td>HomeScienceTools</td><td>CE-SPOTP12</td><td></td></tr><tr><td>96-well microplates</td><td>Greiner Bio-One</td><td>650201</td><td></td></tr><tr><td>Hot plate</td><td>Industrial Equipment and Control Pty Ltd.</td><td>CH1920 (Scientrific)</td><td></td></tr><tr><td>100 mL glass volumetric flasks</td><td>Duran</td><td>24 678 25 54</td><td></td></tr><tr><td>Soda lime glass Pasteur pipettes</td><td>Marienfeld-Superior</td><td>3233050</td><td>230 mm length</td></tr></tbody></table>

color spot test as a presumptive tool for the rapid detection of synthetic cathinones

合成 cathinones は、世界的に法執行機関と他のボーダー保護機関製薬発作でますます普及している新しい精神活性物質 (NPS) の大規模なクラスです。色テストは、迅速かつ単純な化学的方法を使用して特定の薬剤クラスの存在の有無を示す推定同定法です。比較的最近の彼らの出現のおかげで、合成 cathinones の特定の識別のための色テストは現在使用できません。本研究で紹介する合成 cathinones の推定同定のためのプロトコル 3 水溶液、試薬を用いた: 2, 9-ジメチル-1, 10-フェナントロリン (ネオクプロイン) 銅 (ii) 硝酸、酢酸ナトリウム。小さなピンの頭サイズの量 (約 0.1 0.2 mg) スポット磁器の井戸に追加被疑薬剤のプレートと各試薬は、滴下順番にホット プレートで加熱する前に。非常に明るい青から黄色、オレンジに色変更後 10 分は合成 cathinones の可能性の存在を示します。高い安定性特定の試薬法医学研究室で合成 cathinones 未知試料の推定のスクリーニングに使用する可能性があります。ただし、色変更結果の追加加熱ステップの迷惑は研究室のアプリケーションにテストを制限、フィールド テストに簡単に翻訳の可能性が低くなります。

テスト プロトコル Philpらで説明の結果、いくつかの研究で検証されています。17. 色試験方法は推定的色を通して未知試料の合成 cathinones が水色から黄色-オレンジ (図 1) に変更を検出することができます。黄色とオレンジの色は、肯定的なテストの結果と他の色が変わる、非常に弱い黄色を含むまたは heatingare の否定的?...

Watch this Scientific Journal Video about Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones at JoVE.com

Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones

合成 Cathinones の急速な検出のための推定ツールとして色スポット テスト

合成 cathinones、新しい向精神物質のクラスの検出のためのシンプル、安価で、選択的な化学的スポット テスト プロトコルをご紹介します。プロトコルは、違法材が発生する法執行機関の様々 な地域での使用に適しています。

Synthetic cathinones are a large class of new psychoactive substances (NPS) that are increasingly prevalent in drug seizures made by law enforcement and ...

color-spot-test-as-presumptive-tool-for-rapid-detection-synthetic

Universidad de Cartagena UDEC

Research

JoVE Journal

Chemistry

24.6K ビュー.  University of Technology Sydney (UTS). 合成 cathinones、新しい向精神物質のクラスの検出のためのシンプル、安価で、選択的な化学的スポット テスト プロトコルをご紹介します。プロトコルは、違法材が発生する法執行機関の様々 な地域での使用に適しています。

ビデオ: Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Molecular cruciforms are X-shaped systems in which two conjugation axes intersect at a central core. If one axis of these molecules is substituted with electron-donors, and the other with electron-acceptors, cruciforms&#39; HOMO will localize along the electron-rich and LUMO along the electron-poor axis. This spatial isolation of cruciforms&#39; frontier molecular orbitals (FMOs) is essential to their use as sensors, since analyte binding to the cruciform invariably changes its HOMO-LUMO gap and the associated optical properties. Using this principle, Bunz and Miljani&#263; groups developed 1,4-distyryl-2,5-bis(arylethynyl)benzene and benzobisoxazole cruciforms, respectively, which act as fluorescent sensors for metal ions, carboxylic acids, boronic acids, phenols, amines, and anions. The emission colors observed when these cruciform are mixed with analytes are highly sensitive to the details of analyte&#39;s structure and - because of cruciforms&#39; charge-separated excited states - to the solvent in which emission is observed. Structurally closely related species can be qualitatively distinguished within several analyte classes: (a) carboxylic acids; (b) boronic acids, and (c) metals. Using a hybrid sensing system composed from benzobisoxazole cruciforms and boronic acid additives, we were also able to discern among structurally similar: (d) small organic and inorganic anions, (e) amines, and (f) phenols. The method used for this qualitative distinction is exceedingly simple. Dilute solutions (typically 10-6 M) of cruciforms in several off-the-shelf solvents are placed in UV/Vis vials. Then, analytes of interest are added, either directly as solids or in concentrated solution. Fluorescence changes occur virtually instantaneously and can be recorded through standard digital photography using a semi-professional digital camera in a dark room. With minimal graphic manipulation, representative cut-outs of emission color photographs can be arranged into panels which permit quick naked-eye distinction among analytes. For quantification purposes, Red/Green/Blue values can be extracted from these photographs and the obtained numeric data can be statistically processed.

Cross-conjugated cruciform fluorophores based on 1,4-distyryl-2,5-bis(arylethynyl)benzene and benzobisoxazole nuclei can be used to qualitatively identify diverse Lewis acidic and Lewis basic analytes. This method relies on the differences in emission colors of the cruciforms that are observed upon analyte addition. Structurally closely related species can be distinguished from each other.

カルボン酸、ボロン酸、およびアミンの定性的同定は十字フルオロフォアを使用

Molecular cruciforms are X-shaped systems in which two conjugation axes intersect at a central core. If one axis of these molecules is substituted with ...

化学

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

We demonstrate that direct analysis in real time-high resolution mass spectrometry can be used to produce mass spectral profiles of botanical material, and that these chemical fingerprints can be used for plant species identification. The mass spectral data can be acquired rapidly and in a high throughput manner without the need for sample extraction, derivatization or pH adjustment steps. The use of this technique bypasses challenges presented by more conventional techniques including lengthy chromatography analysis times and resource intensive methods. The high throughput capabilities of the direct analysis in real time-high resolution mass spectrometry protocol, coupled with multivariate statistical analysis processing of the data, provide not only class characterization of plants, but also yield species and varietal information. Here, the technique is demonstrated with two psychoactive plant products, Mitragyna speciosa (Kratom) and Datura (Jimsonweed), which were subjected to direct analysis in real time-high resolution mass spectrometry followed by statistical analysis processing of the mass spectral data. The application of these tools in tandem enabled the plant materials to be rapidly identified at the level of variety and species.

A method for species identification of botanical material by direct analysis in real time-high resolution mass spectrometry and multivariate statistical analysis is presented.

リアルタイム高分解能質量分析法で直接分析を用いた植物材料の急速なハイスループット種の同定

We demonstrate that direct analysis in real time-high resolution mass spectrometry can be used to produce mass spectral profiles of botanical material, ...

Fluorescent Paper Strips for the Detection of Diesel Adulteration with Smartphone Read-out

Three fluorescent molecular rotors of 4-dimethylamino-4-nitrostilbene (4-DNS) were investigated for their potential use as viscosity probes to indicate the content of kerosene in diesel/kerosene blends, a wide-spread activity to adulterate fuel. In solvents with low viscosity, the dyes rapidly deactivate via a so-called twisted intramolecular charge transfer state, efficiently quenching the fluorescence. Measurements of diesel/kerosene blends revealed a good linear correlation between the decrease in fluorescence and the increase of the fraction of the less viscous kerosene in diesel/kerosene blends. Immobilization of the hydroxy derivative 4-DNS-OH in cellulose paper yielded test strips that preserve the fluorescent indicator&#39;s behavior. Combination of the strips with a reader based on a smartphone and a controlling app allowed to create a simple field test. The method can reliably detect the presence of kerosene in diesel from 7 to 100%, outperforming present standard methods for diesel adulteration.

Here, we present a protocol to detect the adulteration of diesel with kerosene using test strips coated with a fluorescent viscosity probe together with a smartphone-based analysis system.

スマート フォンの読み出しとディーゼルの粗悪品の検出のための蛍光紙のストリップ

Three fluorescent molecular rotors of 4-dimethylamino-4-nitrostilbene (4-DNS) were investigated for their potential use as viscosity probes to indicate ...

環境学

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

The extraction and analysis of catecholamine neurotransmitters in biological fluids is of great importance in assessing nervous system function and related diseases, but their precise measurement is still a challenge. Many protocols have been described for neurotransmitter measurement by a variety of instruments, including high-pressure liquid chromatography (HPLC). However, there are shortcomings, such as complicated operation or hard-to-detect multiple targets, which cannot be avoided, and presently, the dominant analysis technique is still HPLC coupled with sensitive electrochemical or fluorimetric detection, due to its high sensitivity and good selectivity. Here, a detailed protocol is described for the pretreatment and detection of catecholamines with high pressure liquid chromatography with electrochemical detection (HPLC-ECD) in real urine samples of infants, using electrospun composite nanofibers composed of polymeric crown ether with polystyrene as adsorbent, also known as the packed-fiber solid phase extraction (PFSPE) method. We show how urine samples can be easily precleaned by a nanofiber-packed solid phase column, and how the analytes in the sample can be rapidly enriched, desorbed, and detected on an ECD system. PFSPE greatly simplifies the pretreatment procedures for biological samples, allowing for decreased time, expense, and reduction of the loss of targets. 
Overall, this work illustrates a simple and convenient protocol for solid-phase extraction coupled to an HPLC-ECD system for simultaneous determination of three monoamine neurotransmitters (norepinephrine (NE), epinephrine (E), dopamine (DA)) and two of their metabolites (3-methoxy-4-hydroxyphenylglycol (MHPG) and 3,4-dihydroxy-phenylacetic acid (DOPAC)) in infants' urine. The established protocol was applied to assess the differences of urinary catecholamines and their metabolites between high-risk infants with perinatal brain damage and healthy controls. Comparative analysis revealed a significant difference in urinary MHPG between the two groups, indicating that the catecholamine metabolites may be an important candidate marker for early diagnosis of cases at risk for brain damage in infants.

We present a convenient solid-phase extraction coupled to high-pressure liquid chromatography (HPLC) with electrochemical detection (ECD) for simultaneous determination of three monoamine neurotransmitters and two of their metabolites in infants' urine. We also identify the metabolite MHPG as a potential biomarker for the early diagnosis of brain damage for infants.

抽出と神経伝達物質カテコールアミン及びその代謝物の高速液体クロマトグラフィーによる分析のための便利な方法

The extraction and analysis of catecholamine neurotransmitters in biological fluids is of great importance in assessing nervous system function and ...

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

New analytical methods are urgently needed to enable high-throughput, yet comprehensive drug screening, given an alarming opioid and prescription drug crisis in public health. Conventional urine drug testing based on a two-tier immunoassay screen followed by a gas chromatography-tandem mass spectrometry (GC-MS/MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS) method are expensive and prone to bias while being limited to targeted panels of known drugs of abuse (DoA). Herein, we outline an improved method for drug surveillance that allows for the resolution and detection of an expanded panel of DoA and their metabolites when using multisegment injection-capillary electrophoresis-mass spectrometry (MSI-CE-MS). Multiplexed separations of ten urine samples with a quality control by CE (&lt; 3 min/sample) in conjunction with full-scan data acquisition using a time-of-flight mass spectrometer (TOF-MS) under positive ion mode detection allows for the identification and quantification of DoA above recommended cut-off levels. An excellent resolution of drug isomers and isobars, including background interferences, are achieved when using MSI-CE-MS with an electrokinetic spacer between sample segments, where accurate mass/molecular formula together with the comigration of a matching deuterated internal standard and the detection of one or more bio-transformed metabolites facilitate DoA identification over a wider detection window. Additionally, urine samples can be analyzed directly without enzyme deconjugation for the rapid screening without complicated sample workup. MSI-CE-MS enables the surveillance of a broad spectrum of DoA that is required for the treatment monitoring of high-risk patients, including confirming prescribed drug adherence, revealing illicit drug use/substitution, and evaluating optimal dosage regimes as required for new advances in precision medicine.

Here we describe a high-throughput method for comprehensive drug surveillance that allows for the improved resolution and detection of large panels of drugs of abuse and their metabolites, with quality control based on multisegment injection-capillary electrophoresis-mass spectrometry.

マルチ セグメントの注入キャピラリー電気泳動質量分析法による高スループットかつ包括的な薬物監視

New analytical methods are urgently needed to enable high-throughput, yet comprehensive drug screening, given an alarming opioid and prescription drug ...

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Membrane-based lateral flow immunochromatographic strips (ICSs) are useful tools for low-cost self-diagnosis and have been efficiently applied to toxin, physiological index and clinical biomarker detection. In this protocol, we provide a detailed description of the steps to develop a rapid, sensitive and quantitative lateral-flow immunoassay (using AuNPs as a marker and mAbs as a probe). The procedure describes the preparation and characterization of colloidal gold, synthesis of the AuNP-mAb conjugate, assembly of the immunochromatographic strip, and methodological investigation of the assay. The results showed that the final strips can be further utilized for the rapid and convenient self-diagnosis of a small molecule, which may provide an alternative tool in the rapid and precise analysis of physiological and biological indices.

小分子化合物の迅速・定量的検出用横流イムノクロマトグラフィーストリップの開発

Membrane-based lateral flow immunochromatographic strips (ICSs) are useful tools for low-cost self-diagnosis and have been efficiently applied to toxin, ...

生化学

DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density

Synapses are the site of communication between neurons. Neuronal circuit strength is related to synaptic density, and the breakdown of synapses is characteristic of disease states like major depressive disorder (MDD) and Alzheimer's disease. Traditional techniques to investigate synapse numbers include genetic expression of fluorescent markers (e.g., green fluorescent protein (GFP)), dyes that fill a neuron (e.g., carbocyanine dye, DiI), and immunofluorescent detection of spine markers (e.g., postsynaptic density 95 (PSD95)). A major caveat to these proxy techniques is that they only identify postsynaptic changes. Yet, a synapse is a connection between a presynaptic terminal and a postsynaptic spine. The gold standard for measuring synapse formation/elimination requires time-consuming electron microscopy or array tomography techniques. These techniques require specialized training and costly equipment. Further, only a limited number of neurons can be assessed and are used to represent changes to an entire brain region. DetectSyn is a rapid fluorescent technique that identifies changes to synapse formation or elimination due to a disease state or drug activity. DetectSyn utilizes a rapid proximity ligation assay to detect juxtaposed pre- and postsynaptic proteins and standard fluorescent microscopy, a technique readily available to most laboratories. Fluorescent detection of the resulting puncta allows for quick and unbiased analysis of experiments. DetectSyn provides more representative results than electron microscopy because larger areas can be analyzed than a limited number of fluorescent neurons. Moreover, DetectSyn works for in vitro cultured neurons and fixed tissue slices. Finally, a method is provided to analyze the data collected from this technique. Overall, DetectSyn offers a procedure for detecting relative changes in synapse density across treatments or disease states and is more accessible than traditional techniques.

DetectSyn is an unbiased, rapid fluorescent assay that measures changes in relative synapse (pre- and postsynaptic engagement) number across treatments or disease states. This technique utilizes a proximity ligation technique that can be used both in cultured neurons and fixed tissue.

DetectSyn:シナプス密度の変化を検出するための迅速で偏りのない蛍光法

Synapses are the site of communication between neurons. Neuronal circuit strength is related to synaptic density, and the breakdown of synapses is ...

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

3,4-Methylenedioxymethamphetamine (MDMA; ecstasy) toxicity may cause region-specific changes in serotonergic mRNA expression due to acute serotonin (5-hydroxytryptamine; 5-HT) syndrome. This hypothesis can be tested using in situ hybridization to detect the serotonin 5-HT2A receptor gene htr2a. In the past, such procedures, utilizing radioactive riboprobe, were difficult because of the complicated workflow that needs several days to perform and the added difficulty that the technique required the use of fresh frozen tissues maintained in an RNase-free environment. Recently, the development of short oligonucleotide probes has simplified in situ hybridization procedures and allowed the use of paraformaldehyde-prefixed brain sections, which are more widely available in laboratories. Here, we describe a detailed protocol using non-radioactive oligonucleotide probes on the prefixed brain tissues. Hybridization probes used for this study include dapB (a bacterial gene coding for dihydrodipicolinate reductase), ppiB (a housekeeping gene coding for peptidylprolyl isomerase B), and htr2a (a serotonin gene coding for 5-HT2A receptors). This method is relatively simply, cheap, reproducible and requires less than two days to complete.

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

This protocol describes a rapid and simplified in situ hybridization method ideal forparaformaldehyde-prefixed brain, thus reducing the need for prolonged complex steps while using fresh frozen tissues. The method is validated using the identification of the serotonin 5-HT2A receptor gene htr2a in rats.

ラピッド<em&gt;その場で</emセロトニン症候群のラットのパラホルムアルデヒド接頭辞脳にオリゴヌクレオチドプローブを使用して&gt;ハイブリダイゼーション

3,4-Methylenedioxymethamphetamine (MDMA; ecstasy) toxicity may cause region-specific changes in serotonergic mRNA expression due to acute serotonin ...

神経科学

<meta charset="utf8"></head><body>LC および TIMS-TOF MS/MS のフェンタニル類似体をスクリーニングするための直交性の採取(英語)

Fentanyl Analog Screening using LC-TIMS-TOF MS/MS

The use of fentanyl and the emergence of fentanyl analogs over recent decades has become an increasing concern to the community at large. Fentanyl and its analogs are the major contributors to fatal and nonfatal overdoses in the United States. Most recent cases of fentanyl-related overdose are linked to illicitly manufactured fentanyl and its associated extreme potency. In the present work, we describe a high-throughput analytical protocol for the screening of fentanyl analogs. The use of complementary liquid chromatography, trapped ion mobility spectrometry, and tandem mass spectrometry allow for the separation and assignment of hundreds of fentanyl analogs from a single sample in a single scan. The described approach takes advantage of the recent development of data-dependent acquisition and data-independent acquisition using parallel accumulation in the mobility trap followed by sequential fragmentation using collision-induced dissociation. The fentanyl analogs are confidently assigned based on their retention time, mobility, and MS fragmentation pattern.

<meta charset="utf8"></head><body>著者スポットライト:フェンタニル類似体を自信を持って区別し、特性評価するための効率的な方法論

A method for the screening of fentanyl analogs based on their retention time, mobility, and mass spectrometry fragmentation pattern.

LC-TIMS-TOF MS/MS を用いたフェンタニルアナログスクリーニング

The use of fentanyl and the emergence of fentanyl analogs over recent decades has become an increasing concern to the community at large. Fentanyl and its ...

合成 Cathinones の急速な検出のための推定ツールとして色スポットテスト

要約

さらに動画を探す

この動画の章

カルボン酸、ボロン酸、およびアミンの定性的同定は十字フルオロフォアを使用

リアルタイム高分解能質量分析法で直接分析を用いた植物材料の急速なハイスループット種の同定

スマートフォンの読み出しとディーゼルの粗悪品の検出のための蛍光紙のストリップ

抽出と神経伝達物質カテコールアミン及びその代謝物の高速液体クロマトグラフィーによる分析のための便利な方法

マルチセグメントの注入キャピラリー電気泳動質量分析法による高スループットかつ包括的な薬物監視

小分子化合物の迅速・定量的検出用横流イムノクロマトグラフィーストリップの開発

DetectSyn:シナプス密度の変化を検出するための迅速で偏りのない蛍光法

ラピッド

LC-TIMS-TOF MS/MS を用いたフェンタニルアナログスクリーニング

LC-TIMS-TOF MS/MS を用いたフェンタニルアナログスクリーニング

合成 Cathinones の急速な検出のための推定ツールとして色スポット テスト

要約

さらに動画を探す

この動画の章

カルボン酸、ボロン酸、およびアミンの定性的同定は十字フルオロフォアを使用

リアルタイム高分解能質量分析法で直接分析を用いた植物材料の急速なハイスループット種の同定

スマート フォンの読み出しとディーゼルの粗悪品の検出のための蛍光紙のストリップ

抽出と神経伝達物質カテコールアミン及びその代謝物の高速液体クロマトグラフィーによる分析のための便利な方法

マルチ セグメントの注入キャピラリー電気泳動質量分析法による高スループットかつ包括的な薬物監視

小分子化合物の迅速・定量的検出用横流イムノクロマトグラフィーストリップの開発

DetectSyn:シナプス密度の変化を検出するための迅速で偏りのない蛍光法

ラピッド

LC-TIMS-TOF MS/MS を用いたフェンタニルアナログスクリーニング

LC-TIMS-TOF MS/MS を用いたフェンタニルアナログスクリーニング

合成 Cathinones の急速な検出のための推定ツールとして色スポットテスト

スマートフォンの読み出しとディーゼルの粗悪品の検出のための蛍光紙のストリップ

マルチセグメントの注入キャピラリー電気泳動質量分析法による高スループットかつ包括的な薬物監視