私たちは、自然科学とカナダの工学研究評議会（NSERC、＃436117から2013）、癌研究学会（CRS、＃20172）、骨髄腫カナダ、グランドチャレンジカナダからの財政支援を認めます。

以下で説明する手順のすべてを開発し、非クリーンルーム環境で行われていることに注意してください。溶剤結合ステップは、利用可能な場合に必ず、クリーンルームで行うことができるが、これは必須ではありません。 熱可塑性マイクロ流体デバイス層の調製<ol><li>設計および適切な製造方法を用いて、選択した熱可塑性のマイクロ流体デバイス層を作製する（ 例えば 、16エンボス、15マイクロミリング- 18、射出成形）。 </li><li>視覚的にエッジが「クリーン」であることを確認するために、デバイス層を検査する（ すなわち 、製造工程から無バリや残りの材料の隆起部）。最良の結果を得るために、光学顕微鏡下でデバイスの外側のエッジに加えて、すべての機械加工されたマイクロフィーチャエッジをチェックしてください。 </li><li>残りの材料を目視検査中に発見された場合、カミソリの刃を使用し、またはメスを十分に行い、マットを削除するには層の界面がコンフォーマルに接触するように互いに対して平らに横たわっているからデバイス層を防ぎerial。 </li><li>クリーンデバイスは、実験室の石鹸と水と圧縮空気で乾燥して表面。 2分間の2-プロパノールにデバイス層を水没し、圧縮空気で乾燥しました。 </li></ol> 2.溶剤接着<ol><li> 加熱（PMMA用）プレス又は（COP用）ホットプレートを準備します。 <ol><li> PMMA（キャストアクリル、〜100〜110℃のガラス転移温度）18予熱プレスを70℃、そして温度を安定させます。 </li><li> （メーカーから102℃のガラス転移温度）COPのために、25℃にホットプレートを予熱し、温度が安定します。 </li></ol></li><li> ボンディング工程のための溶媒準備します。 <ol><li> PMMAのために、接合面積の平方インチ当たり0.5mlのエタノールを測定します。 </li><li> COPは、2-プロパノール、シクロヘキサン、ウィットの65:35混合物を調製接合面積の平方インチ当たりの混合物を0.5mlのHA全容積。 注：シクロヘキサンは、一般的なポリプロピレンの実験器具を溶解するようCOPについては、ガラスピペットとコンテナを使用します。シクロヘキサンは有毒であるとして、ヒュームフード内のすべての混合との結合を実行します。 </li></ol></li><li> 清浄プラスチック層の間の接着面積の平方インチ当たり溶媒を0.1ml分配し、層を一緒にもたらします。視覚的に共通であり、可能な限り除去する必要があり、接合界面での気泡、点検します。 注：揮発性溶媒が蒸発を開始します（したがって、溶媒の混合物が組成物に変更されます）溶媒としては、分配された後に迅速に作業することが有益です。 <ol><li>気泡が存在する場合、それらはほぼ離れて来るように、接合界面に沿った2つのプラスチック層をスライドさせ（ただし、接触したまま）、その後、戻って一緒にそれらをスライドさせます。 </li></ol></li><li> 位置合わせピンとデバイスの層を合わせ、カスタム治具、あるいは単に手で（詳細については、議論のセクションを参照）。 <ol><li>位置合わせピンを使用している場合、ピン用の穴を合わせて、デバイススタックにピンを挿入します。 </li><li>カスタム治具を使用している場合は、治具にデバイススタックを挿入し、装置の周囲で締めます。 </li><li>手で揃える場合は、デバイスの外側のエッジを揃えるために指を使用しています。 </li></ol></li><li> （PMMA）または（COP用）予備加熱したホットプレート上に予備加熱したプレス内に溶剤を持つデバイスを配置します。 <ol><li> PMMAの場合は、2分間の圧力の2300キロパスカルを適用します。 </li><li> COPは、圧力の350キロパスカルを適用します。 5℃/分の速度で70℃まで25℃の温度を上げます。 70°C（10分後）に到達した後、さらに15分間の結合。 </li></ol></li><li>安全検査のためのホットデバイスを削除するには、ピンセットを使用します。接合が完了しました。 </li><li> マイクロチャネルまたは他のfeaturで（デバイスに残っている液体を除去エス）。 <ol><li> PMMAの場合は、圧縮空気で、残りの液体を除去します。 COPは、任意の残りのシクロヘキサンを除去するために、24時間45℃でホットプレートやベークに貼り付けデバイスを配置します。 </li></ol></li></ol>

<ol>
	<li>Beebe, D. J., Mensing, G. A., Walker, G. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Physics+and+applications+of+microfluidics+in+biology.">Physics and applications of microfluidics in biology.</a> Annual Review of Biomedical Engineering. 4, 261-286 (2002).</li><li>Situma, C., Hashimoto, M., Soper, S. a. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Merging+microfluidics+with+microarray-based+bioassays.">Merging microfluidics with microarray-based bioassays.</a> Biomolecular Engineering. 23 (5), 213-231 (2006).</li><li>Paguirigan, A. L., Beebe, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microfluidics+meet+cell+biology:+Bridging+the+gap+by+validation+and+application+of+microscale+techniques+for+cell+biological+assays.">Microfluidics meet cell biology: Bridging the gap by validation and application of microscale techniques for cell biological assays.</a> BioEssays. 30 (9), 811-821 (2008).</li><li>Young, E. W. K., Beebe, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fundamentals+of+microfluidic+cell+culture+in+controlled+microenvironments.">Fundamentals of microfluidic cell culture in controlled microenvironments.</a> Chemical Society Reviews. 39 (3), 1036-1048 (2010).</li><li>Duffy, D. C., McDonald, J. C., Schueller, O. J. A., Whitesides, G. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapid+Prototyping+of+Microfluidic+Systems+in+Poly(dimethylsiloxane).">Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane).</a> Analytical Chemistry. 70 (23), 4974-4984 (1998).</li><li>Berthier, E., Young, E. W. K., Beebe, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Engineers+are+from+PDMS-land,+Biologists+are+from+Polystyrenia.">Engineers are from PDMS-land, Biologists are from Polystyrenia.</a> Lab on a Chip. 12 (7), 1224-1237 (2012).</li><li>Sackmann, E. K., Fulton, A. L., Beebe, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+present+and+future+role+of+microfluidics+in+biomedical+research.">The present and future role of microfluidics in biomedical research.</a> Nature. 507 (7491), 181-189 (2014).</li><li>Young, E. W. K., Berthier, E., Beebe, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+enhanced+autofluorescence+and+impact+on+cell+microscopy+for+microfabricated+thermoplastic+devices.">Assessment of enhanced autofluorescence and impact on cell microscopy for microfabricated thermoplastic devices.</a> Analytical Chemistry. 85 (1), 44-49 (2013).</li><li>Wallow, T. I., Morales, A. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Low-distortion,+high-strength+bonding+of+thermoplastic+microfluidic+devices+employing+case-II+diffusion-mediated+permeant+activation.">Low-distortion, high-strength bonding of thermoplastic microfluidic devices employing case-II diffusion-mediated permeant activation.</a> Lab on a Chip. 7 (12), 1825-1831 (2007).</li><li>Tsao, C. W., DeVoe, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bonding+of+thermoplastic+polymer+microfluidics.">Bonding of thermoplastic polymer microfluidics.</a> Microfluidics and Nanofluidics. 6 (1), 1-16 (2009).</li><li>Young, E. W. K., Berthier, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapid+prototyping+of+arrayed+microfluidic+systems+in+polystyrene+for+cell-based+assays.">Rapid prototyping of arrayed microfluidic systems in polystyrene for cell-based assays.</a> Analytical Chemistry. 83 (4), 1408-1417 (2011).</li><li>Truckenm&#252;ller, R., Henzi, P., Herrmann, D., Saile, V., Schomburg, W. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bonding+of+polymer+microstructures+by+UV+irradiation+and+subsequent+welding+at+low+temperatures.">Bonding of polymer microstructures by UV irradiation and subsequent welding at low temperatures.</a> Microsystem Technologies. 10 (5), 372-374 (2004).</li><li>Tsao, C. W., Hromada, L., Liu, J., Kumar, P., DeVoe, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Low+temperature+bonding+of+PMMA+and+COC+microfluidic+substrates+using+UV/ozone+surface+treatment.">Low temperature bonding of PMMA and COC microfluidic substrates using UV/ozone surface treatment.</a> Lab on a Chip. 7 (4), 499-505 (2007).</li><li>Wan, A. M. D., Sadri, A., Young, E. W. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Liquid+phase+solvent+bonding+of+plastic+microfluidic+devices+assisted+by+retention+grooves.">Liquid phase solvent bonding of plastic microfluidic devices assisted by retention grooves.</a> Lab on a Chip. 15 (18), 3785-3792 (2015).</li><li>Guckenberger, D. J., de Groot, T. E., Wan, A. M. D., Beebe, D. J., Young, E. W. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Micromilling:+a+method+for+ultra-rapid+prototyping+of+plastic+microfluidic+devices.">Micromilling: a method for ultra-rapid prototyping of plastic microfluidic devices.</a> Lab on a Chip. 15 (11), 2364-2378 (2015).</li><li>Cameron, N. S., Roberge, H., Veres, T., Jakeway, S. C., John Crabtree, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+fidelity,+high+yield+production+of+microfluidic+devices+by+hot+embossing+lithography:+rheology+and+stiction.">High fidelity, high yield production of microfluidic devices by hot embossing lithography: rheology and stiction.</a> Lab on a Chip. 6 (7), 936 (2006).</li><li>Yang, S., Devoe, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microfluidic+device+fabrication+by+thermoplastic+hot-embossing.">Microfluidic device fabrication by thermoplastic hot-embossing.</a> Methods in Molecular Biology. 949, 115-123 (2013).</li><li>Konstantinou, D., Shirazi, A., Sadri, A., Young, E. W. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Combined+hot+embossing+and+milling+for+medium+volume+production+of+thermoplastic+microfluidic+devices.">Combined hot embossing and milling for medium volume production of thermoplastic microfluidic devices.</a> Sensors and Actuators B: Chemical. 234, 209-221 (2016).</li><li>Maszara, W. P., Goetz, G., Caviglia, A., McKitterick, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bonding+of+silicon+wafers+for+silicon-on-insulator.">Bonding of silicon wafers for silicon-on-insulator.</a> Journal of Applied Physics. 64 (10), 4943 (1988).</li><li>Bhattacharyya, A., Klapperich, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanical+and+chemical+analysis+of+plasma+and+ultraviolet-ozone+surface+treatments+for+thermal+bonding+of+polymeric+microfluidic+devices.">Mechanical and chemical analysis of plasma and ultraviolet-ozone surface treatments for thermal bonding of polymeric microfluidic devices.</a> Lab on a Chip. 7 (7), 876-882 (2007).</li></ol>

著者は、彼らが競合する金融利害関係を持たないことを宣言します。

潜在的なボンディング戦略の実現可能性は、利用可能な機器に依存します。ホットプレートは、比較的一般的であり、フリーウェイトが安価に購入することができますが、高圧戦略は、加熱プレス機の使用が必要になります。例えば、我々の最適なPMMA結合レシピエタノール（ 表1参照）と結合するために高い圧力を必要とし、必要な圧力は、フリーウェイトを使用する典型的な?...

4 -マイクロフルイディクスは、マイクロスケール1で、大幅生物学研究に2を貢献する成長約束で化学と物理学を研究するのに適し技術として、過去20年間に浮上しています。マイクロ流体デバイスの大部分は、歴史的に、（PDMS）、ポリ（ジメチルシロキサン）から、使いやすく安価なシリコーンエラストマーを行い、高品質の特徴の複製5が提供されています。しかし、PDMSはよく文書欠点を有しており、高容量の製造は6,7を処理すると互換性がありません、そのようなものとして、ため大量生産、したがって、商業化のための彼らの潜在的なのは、熱可塑性材料からマイクロ流体デバイスを作製するに向かって成長している傾向がありました。 プラスチック微細加工の広い普及に対する大きな障壁の一つは、プラスチック装置の容易な、高品質の接合を達成されています。現在の戦略はトンを採用しますhermal、接着剤、溶媒結合技術は、多くの重要な課題に悩まされます。接着剤技術はステンシル、慎重な位置合わせを必要とし、最終的にマイクロ流路10に露出した接着剤の厚さを残しながら、11 -熱結合は自己蛍光8が増大し、多くの場合、マイクロチャネルの形状9を変形させます。 14 -溶剤接着は、そのシンプルさ、同調性、および低コスト10,12に魅力的です。具体的には、その同調性は、マイクロ構造14の変形を最小限に抑え、一貫性のある高品質な接合を得ることができる様々なプラスチックのための最適化を可能にします。 溶剤接合時、溶媒露出が接合界面を横切る鎖の相互拡散を可能にする、プラスチックの表面付近のポリマー鎖の移動度を増大させます。これは、拡散チェーンの機械的連結を介して絡み合いを生じ、およびAPにおける結果hysicalボンド10。熱接合は、同様に機能するが、連鎖移動性を増加させるだけで、高温に依存します。溶媒の使用が大幅に結合するために必要な温度を低下させる、従って、不要な変形を低減することができ、一方、このように、熱的方法は、ポリマーのガラス転移点付近またはそれ以上の温度を必要とします。 私たちは、PMMAとCOPデバイスの両方を結合するための特定のプロトコルを提供します。しかしながら、このプロトコルおよび方法は、溶媒、他のプラスチック材料に適合させることができる熱可塑性マイクロ流体デバイスのボンディング、溶剤、および利用可能な機器のための簡単な、一般的な方法を記載しています。私たちは、債券の品質を評価するための多数の方法を説明する（ 例えば 、結合カバレッジ、接着強度、接着耐久性、及び微細形状の変形）、およびこれらの共通の課題に対処するためのトラブルシューティングのアプローチを提供します。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>COP</td>
			<td>Zeonor</td>
			<td>604Z1020R080</td>
			<td>20 kg COP Pellets - 1020R. Multiple suppliers can be used, but may affect bonding characteristics.</td>
		</tr>
		<tr>
			<td>PMMA</td>
			<td>McMaster Carr</td>
			<td>8560K173</td>
			<td>1.5 mm sheet thickness for our typical applications. Multiple suppliers can be used, but may affect bonding characteristics.</td>
		</tr>
		<tr>
			<td>Cyclohexane</td>
			<td>Sigma-Aldrich</td>
			<td>227048</td>
			<td>Cyclohexane, anhydrous, 99.5%. Multiple suppliers can be used. Toxic, requires fumehood.</td>
		</tr>
		<tr>
			<td>Ethanol</td>
			<td>Sigma-Aldrich</td>
			<td>24102</td>
			<td>Ethanol, absolute, &#8805;99.8% (GC). Multiple suppliers can be used.</td>
		</tr>
		<tr>
			<td>Acetone</td>
			<td>Sigma-Aldrich</td>
			<td>179124</td>
			<td>Acetone, ACS reagent, &#8805;99.5%. Multiple suppliers can be used.</td>
		</tr>
		<tr>
			<td>2-Propanol</td>
			<td>Sigma-Aldrich</td>
			<td>278475</td>
			<td>2-Propanol, anhydrous, 99.5%. Multiple suppliers can be used.</td>
		</tr>
		<tr>
			<td>Hot plate(s)</td>
			<td>Torrey Pines Scientific</td>
			<td>HP60</td>
			<td>Fully programmable digital hotplate. Multiple suppliers can be used.</td>
		</tr>
		<tr>
			<td>Free weights</td>
			<td>Cap Barbell</td>
			<td>RPG#2</td>
			<td>Standard cast iron plate. Multiple suppliers and different weights can be used.</td>
		</tr>
		<tr>
			<td>Heated press</td>
			<td>Carver</td>
			<td>Auto CH</td>
			<td>Auto series heated hydraulic press. Multiple suppliers can be used. A press that fits in a fumehood would allow the most flexibility (this model does not).</td>
		</tr>
		<tr>
			<td>CNC Milling Machine</td>
			<td>Tormach</td>
			<td>PCNC 770</td>
			<td>3 Axis CNC mill. Multiple suppliers can be used.</td>
		</tr>
		<tr>
			<td>Endmills</td>
			<td>Various</td>
			<td>Various</td>
			<td>Required sizes depend on designs. Multiple suppliers can be used.</td>
		</tr>
	</tbody>
</table>

solvent bonding for fabrication of pmma and cop microfluidic devices

熱可塑性マイクロ流体デバイスは、シリコーンエラストマーから作られたものよりも多くの利点を提供していますが、ボンディング手順は、関心のある各熱可塑性のために開発されなければなりません。溶剤接着は、プラスチックの様々なデバイスを製造するために使用することができる単純で汎用性の高い方法です。適当な溶媒は、接合すべき2つのデバイス層の間に追加され、熱および圧力は、ボンディングを容易にするために、デバイスに適用されます。溶媒、プラスチック、熱及び圧力の適切な組み合わせを使用することにより、装置は、高品質の結合で密封することができ、微細に高い接着被覆、接着強度、光学的透明性、経時的耐久性、及び低変形または損傷を有することを特徴とジオメトリ。我々は、接合2つの一般的な熱可塑性樹脂から作られたデバイス、ポリ（メチルメタクリレート）（PMMA）、およびシクロオレフィンポリマー（COP）、ならびに得られた結合の品質を特徴付けるための種々の方法、および戦略のための手順を記述しトゥルへ低品質の結合をbleshoot。これらの方法は、他のプラスチック、溶媒系のための新たな溶剤結合プロトコルを開発するために使用することができます。

一般的な溶剤結合手順の概略を図1に示されています。接合品質を評価するための最も簡単な方法は、貧弱な結合カバレッジは未結合のプラスチックの領域のように簡単に表示されているので、視覚的に、債券のカバレッジを検査することであり、弱い結合の指標です。このような領域は、典型的には、近くの自由端（ 例えば 、デバイスの周辺、?...

Watch this Scientific Journal Video about Solvent Bonding for Fabrication of PMMA and COP Microfluidic Devices at JoVE.com

Solvent Bonding for Fabrication of PMMA and COP Microfluidic Devices

溶剤結合は、高品質の結合を有する熱可塑性マイクロ流体デバイスを製造するための簡単で汎用性の高い方法です。私たちは、PMMAに強い、光学的に透明な債券や圧力、温度、適切な溶媒、およびデバイスの幾何学的形状の賢明な組み合わせによって、微細の詳細を保存するCOPマイクロ流体デバイスを実現するためのプロトコルについて説明します。

PMMAおよびCOPマイクロ流体デバイスの作製のための溶剤接着

Thermoplastic microfluidic devices offer many advantages over those made from silicone elastomers, but bonding procedures must be developed for each ...

solvent-bonding-for-fabrication-of-pmma-and-cop-microfluidic-devices

Universidad de Cartagena UDEC

Research

JoVE Journal

Engineering

16.1K ビュー.  University of Toronto. 溶剤結合は、高品質の結合を有する熱可塑性マイクロ流体デバイスを製造するための簡単で汎用性の高い方法です。私たちは、PMMAに強い、光学的に透明な債券や圧力、温度、適切な溶媒、およびデバイスの幾何学的形状の賢明な組み合わせによって、微細の詳細を保存するCOPマイクロ流体デバイスを実現するためのプロトコルについて説明します。 

ビデオ: Solvent Bonding for Fabrication of PMMA and COP Microfluidic Devices

Fabrication of Uniform Nanoscale Cavities via Silicon Direct Wafer Bonding

Measurements of the heat capacity and superfluid fraction of confined 4He have been performed near the lambda transition using lithographically patterned and bonded silicon wafers. Unlike confinements in porous materials often used for these types of experiments3, bonded wafers provide predesigned uniform spaces for confinement. The geometry of each cell is well known, which removes a large source of ambiguity in the interpretation of data.
Exceptionally flat, 5 cm diameter, 375 &#181;m thick Si wafers with about 1 &#181;m variation over the entire wafer can be obtained commercially (from Semiconductor Processing Company, for example). Thermal oxide is grown on the wafers to define the confinement dimension in the z-direction. A pattern is then etched in the oxide using lithographic techniques so as to create a desired enclosure upon bonding. A hole is drilled in one of the wafers (the top) to allow for the introduction of the liquid to be measured. The wafers are cleaned2 in RCA solutions and then put in a microclean chamber where they are rinsed with deionized water4. The wafers are bonded at RT and then annealed at ~1,100 &#176;C. This forms a strong and permanent bond. This process can be used to make uniform enclosures for measuring thermal and hydrodynamic properties of confined liquids from the nanometer to the micrometer scale.

A method for permanently bonding two silicon wafers so as to realize a uniform enclosure is described. This includes wafer preparation, cleaning, RT bonding, and annealing processes. The resulting bonded wafers (cells) have uniformity of enclosure ~1%1,2. The resulting geometry allows for measurements of confined liquids and gasses.

シリコン直接ウェハー接合による均一ナノスケールキャビティの作製

Measurements of the heat capacity and superfluid fraction of confined 4He have been performed near the lambda transition using lithographically patterned ...

工学

Fabrication and Testing of Microfluidic Optomechanical Oscillators

Cavity optomechanics experiments that parametrically couple the phonon modes and photon modes have been investigated in various optical systems including microresonators. However, because of the increased acoustic radiative losses during direct liquid immersion of optomechanical devices, almost all published optomechanical experiments have been performed in solid phase. This paper discusses a recently introduced hollow microfluidic optomechanical resonator. Detailed methodology is provided to fabricate these ultra-high-Q microfluidic resonators, perform optomechanical testing, and measure radiation pressure-driven breathing mode and SBS-driven whispering gallery mode parametric vibrations. By confining liquids inside the capillary resonator, high mechanical- and optical- quality factors are simultaneously maintained.

Parametric optomechanical excitations have recently been experimentally demonstrated in microfluidic optomechanical resonators by means of optical radiation pressure and stimulated Brillouin scattering. This paper describes the fabrication of these microfluidic resonators along with methodologies for generating and verifying optomechanical oscillations.

作製とマイクロ流体光力学発振器のテスト

Cavity optomechanics experiments that parametrically couple the phonon modes and photon modes have been investigated in various optical systems including ...

Fabrication of Gate-tunable Graphene Devices for Scanning Tunneling Microscopy Studies with Coulomb Impurities

Owing to its relativistic low-energy charge carriers, the interaction between graphene and various impurities leads to a wealth of new physics and degrees of freedom to control electronic devices. In particular, the behavior of graphene&#8217;s charge carriers in response to potentials from charged Coulomb impurities is predicted to differ significantly from that of most materials. Scanning tunneling microscopy (STM) and scanning tunneling spectroscopy (STS) can provide detailed information on both the spatial and energy dependence of graphene&#39;s electronic structure in the presence of a charged impurity.&#160;The design of a hybrid impurity-graphene device, fabricated using controlled deposition of impurities onto a back-gated graphene surface, has enabled several novel methods for controllably tuning graphene&#8217;s electronic properties.1-8 Electrostatic gating enables control of the charge carrier density in graphene and the ability to reversibly tune the charge2 and/or molecular5 states of an impurity. This paper outlines the process of fabricating a gate-tunable graphene device decorated with individual Coulomb impurities for combined STM/STS studies.2-5 These studies provide valuable insights into the underlying physics, as well as signposts for designing hybrid graphene devices.

This paper details the fabrication process of a gate-tunable graphene device, decorated with Coulomb impurities for scanning tunneling microscopy studies. Mapping the spatially dependent electronic structure of graphene in the presence of charged impurities unveils the unique behavior of its relativistic charge carriers in response to a local Coulomb potential.

クーロン不純物を走査トンネル顕微鏡研究のためのゲート - 調整可能なグラフェンデバイスの作製

Owing to its relativistic low-energy charge carriers, the interaction between graphene and various impurities leads to a wealth of new physics and degrees ...

Thermal Measurement Techniques in Analytical Microfluidic Devices

Thermal measurement techniques have been used for many applications such as thermal characterization of materials and chemical reaction detection. Micromachining techniques allow reduction of the thermal mass of fabricated structures and introduce the possibility to perform high sensitivity thermal measurements in the micro-scale and nano-scale devices. Combining thermal measurement techniques with microfluidic devices allows performing different analytical measurements with low sample consumption and reduced measurement time by integrating the miniaturized system on a single chip. The procedures of thermal measurement techniques for particle detection, material characterization, and chemical detection are introduced in this paper.

Here, we present three protocols for thermal measurements in microfluidic devices.

分析マイクロ流体デバイスの熱測定技術

Thermal measurement techniques have been used for many applications such as thermal characterization of materials and chemical reaction detection. ...

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

Acoustophoresis refers to the displacement of suspended objects in response to directional forces from sound energy. Given that the suspended objects must be smaller than the incident wavelength of sound and the width of the fluidic channels are typically tens to hundreds of micrometers across, acoustofluidic devices typically use ultrasonic waves generated from a piezoelectric transducer pulsating at high frequencies (in the megahertz range). At characteristic frequencies that depend on the geometry of the device, it is possible to induce the formation of standing waves that can focus particles along desired fluidic streamlines within a bulk flow. Here, we describe a method for the fabrication of acoustophoretic devices from common materials and clean room equipment. We show representative results for the focusing of particles with positive or negative acoustic contrast factors, which move towards the pressure nodes or antinodes of the standing waves, respectively. These devices offer enormous practical utility for precisely positioning large numbers of microscopic entities (e.g., cells) in stationary or flowing fluids for applications ranging from cytometry to assembly.

Acoustofluidic devices use ultrasonic waves within microfluidic channels to manipulate, concentrate and isolate suspended micro and nanoscopic entities. This protocol describes the fabrication and operation of such a device supporting bulk acoustic standing waves to focus particles in a central streamline without the aid of sheath fluids.

作製とAcoustofluidicのデバイスの動作は、粒子のフォーカシングSheathlessためのバルク弾性定在波を支援

Acoustophoresis refers to the displacement of suspended objects in response to directional forces from sound energy. Given that the suspended objects must ...

Fabrication of Fully Solution Processed Inorganic Nanocrystal Photovoltaic Devices

We demonstrate a method for the preparation of fully solution processed inorganic solar cells from a spin and spray coating deposition of nanocrystal inks. For the photoactive absorber layer, colloidal CdTe and CdSe nanocrystals (3-5 nm) are synthesized using an inert hot injection technique and cleaned with precipitations to remove excess starting reagents. Similarly, gold nanocrystals (3-5 nm) are synthesized under ambient conditions and dissolved in organic solvents. In addition, precursor solutions for transparent conductive indium tin oxide (ITO) films are prepared from solutions of indium and tin salts paired with a reactive oxidizer. Layer-by-layer, these solutions are deposited onto a glass substrate following annealing (200-400 &#176;C) to build the nanocrystal solar cell (glass/ITO/CdSe/CdTe/Au). Pre-annealing ligand exchange is required for CdSe and CdTe nanocrystals where films are dipped in NH4Cl:methanol to replace long-chain native ligands with small inorganic Cl- anions. NH4Cl(s) was found to act as a catalyst for the sintering reaction (as a non-toxic alternative to the conventional CdCl2(s) treatment) leading to grain growth (136&#177;39 nm) during heating. The thickness and roughness of the prepared films are characterized with SEM and optical profilometry. FTIR is used to determine the degree of ligand exchange prior to sintering, and XRD is used to verify the crystallinity and phase of each material. UV/Vis spectra show high visible light transmission through the ITO layer and a red shift in the absorbance of the cadmium chalcogenide nanocrystals after thermal annealing. Current-voltage curves of completed devices are measured under simulated one sun illumination. Small differences in deposition techniques and reagents employed during ligand exchange have been shown to have a profound influence on the device properties. Here, we examine the effects of chemical (sintering and ligand exchange agents) and physical treatments (solution concentration, spray-pressure, annealing time and annealing temperature) on photovoltaic device performance.

This protocol describes the synthesis and solution deposition of inorganic nanocrystals layer by layer to produce thin film electronics on non-conductive surfaces. Solvent-stabilized inks can produce complete photovoltaic devices on glass substrates via spin and spray coating following post-deposition ligand exchange and sintering.

完全に解決策が処理無機ナノ結晶太陽電池素子の作製

We demonstrate a method for the preparation of fully solution processed inorganic solar cells from a spin and spray coating deposition of nanocrystal ...

Scanning Light Scattering Profiler (SLPS) Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses

The scanning light scattering profiler (SLSP) methodology has been developed for the full-angle quantitative evaluation of forward and backward light scattering from intraocular lenses (IOLs) using goniophotometer principles. This protocol describes the SLSP platform and how it employs a 360&#176; rotational photodetector sensor that is scanned around an IOL sample while recording the intensity and location of scattered light as it passes through the IOL medium. The SLSP platform can be used to predict, non-clinically, the propensity for current and novel IOL designs and materials to induce light scatter. Non-clinical evaluation of light scattering properties of IOLs can significantly reduce the number of patient complaints related to unwanted glare, glistening, optical defects, poor image quality, and other phenomena associated with the unintended light scattering. Future studies should be conducted to correlate SLSP data with clinical results to help identify which measured light scatter is most problematic for patients that have undergone cataract surgery subsequent to IOL implantation.

Scanning Light Scattering Profiler SLPS Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses

This protocol describes the scanning light scattering profiler (SLSP) that enables the full-angle quantitative evaluation of forward and backward scattering of light from intraocular lenses (IOLs) using goniophotometer principles.

眼内レンズからの前方散乱光および後方散乱光を定量的に評価するための走査型光散乱プロファイラ（SLPS）に基づく方法論

The scanning light scattering profiler (SLSP) methodology has been developed for the full-angle quantitative evaluation of forward and backward light ...

Flow-assisted Dielectrophoresis: A Low Cost Method for the Fabrication of High Performance Solution-processable Nanowire Devices

Flow-assisted dielectrophoresis (DEP) is an efficient self-assembly method for the controllable and reproducible positioning, alignment, and selection of nanowires. DEP is used for nanowire analysis, characterization, and for solution-based fabrication of semiconducting devices. The method works by applying an alternating electric field between metallic electrodes. The nanowire formulation is then dropped onto the electrodes which are on an inclined surface to create a flow of the formulation using gravity. The nanowires then align along the gradient of the electric field and in the direction of the liquid flow. The frequency of the field can be adjusted to select nanowires with superior conductivity and lower trap density. 
In this work, flow-assisted DEP is used to create nanowire field effect transistors. Flow-assisted DEP has several advantages: it allows selection of nanowire electrical properties; control of nanowire length; placement of nanowires in specific areas; control of orientation of nanowires; and control of nanowire density in the device. 
The technique can be expanded to many other applications such as gas sensors and microwave switches. The technique is efficient, quick, reproducible, and it uses a minimal amount of dilute solution making it ideal for the testing of novel nanomaterials. Wafer scale assembly of nanowire devices can also be achieved using this technique, allowing large numbers of samples for testing and large-area electronic applications.

In this paper, flow assisted dielectrophoresis is demonstrated for the self-assembly of nanowire devices. The fabrication of a silicon nanowire field effect transistor is shown as an example.

誘電泳動の流れ支援: 高性能低温硬化型ナノワイヤ デバイス作製のための低コストの方法

Flow-assisted dielectrophoresis (DEP) is an efficient self-assembly method for the controllable and reproducible positioning, alignment, and selection of ...

Atmospheric Pressure Fabrication of Large-Sized Single-Layer Rectangular SnSe Flakes

Tin selenide (SnSe) belongs to the family of layered metal chalcogenide materials with a buckled structure like phosphorene, and has shown potential for applications in two-dimensional nanoelectronics devices. Although many methods to synthesize SnSe nanocrystals have been developed, a simple way to fabricate large-sized single-layer SnSe flakes remains a great challenge. Herein, we show the experimental method to directly grow large-sized single-layer rectangular SnSe flakes on commonly used SiO2/Si insulating substrates using a straightforward two-step fabrication method in an atmospheric pressure quartz tube furnace system. The single-layer rectangular SnSe flakes with an average thickness of ~6.8 &#197; and lateral dimensions of about 30 &#181;m &#215; 50 &#181;m were fabricated by a combination of vapor transport deposition technique and nitrogen etching route. We characterized the morphology, microstructure, and electrical properties of the rectangular SnSe flakes and obtained excellent crystallinity and good electronic properties. This article about the two-step fabrication method can help researchers grow other similar two-dimensional, large-sized, single-layer materials using an atmospheric pressure system.

A protocol is presented demonstrating a two-step fabrication technique to grow large-sized single-layer rectangular shaped SnSe flakes on low-cost SiO2/Si dielectrics wafers in an atmospheric pressure quartz tube furnace system.

大型単層長方形 SnSe フレークの大気圧作製

Tin selenide (SnSe) belongs to the family of layered metal chalcogenide materials with a buckled structure like phosphorene, and has shown potential for ...

Fabrication of Refractive-index-matched Devices for Biomedical Microfluidics

The use of microfluidic devices has emerged as a defining tool for biomedical applications. When combined with modern microscopy techniques, these devices can be implemented as part of a robust platform capable of making simultaneous complementary measurements. The primary challenge created by the combination of these two techniques is the mismatch in refractive index between the materials traditionally used to make microfluidic devices and the aqueous solutions typically used in biomedicine. This mismatch can create optical artifacts near the channel or device edges. One solution is to reduce the refractive index of the material used to fabricate the device by using a fluorinated polymer such as MY133-V2000 whose refractive index is similar to that of water (n = 1.33). Here, the construction of a microfluidic device made out of MY133-V2000 using soft lithography techniques is demonstrated, using O2 plasma in conjunction with an acrylic holder to increase the adhesion between the MY133-V2000 fabricated device and the polydimethylsiloxane (PDMS) substrate. The device is then tested by incubating it filled with cell culture media for 24 h to demonstrate the ability of the device to maintain cell culture conditions during the course of a typical imaging experiment. Finally, quantitative phase microscopy (QPM) is used to measure the distribution of mass within the live adherent cells in the microchannel. This way, the increased precision, enabled by fabricating the device from a low index of refraction polymer such as MY133-V2000 in lieu of traditional soft lithography materials such as PDMS, is demonstrated. Overall, this approach for fabricating microfluidic devices can be readily integrated into existing soft lithography workflows in order to reduce optical artifacts and increase measurement precision.

This protocol describes the fabrication of microfluidic devices from MY133-V2000 to eliminate artifacts that often arise in microchannels due to the mismatching refractive indices between microchannel structures and an aqueous solution. This protocol uses an acrylic holder to compress the encapsulated device, improving adhesion both chemically and mechanically.

生体の屈折率-屈折率整合素子の作製

The use of microfluidic devices has emerged as a defining tool for biomedical applications. When combined with modern microscopy techniques, these devices ...

PMMAおよびCOPマイクロ流体デバイスの作製のための溶剤接着

要約

さらに動画を探す

この動画の章

シリコン直接ウェハー接合による均一ナノスケールキャビティの作製

作製とマイクロ流体光力学発振器のテスト

クーロン不純物を走査トンネル顕微鏡研究のためのゲート - 調整可能なグラフェンデバイスの作製

分析マイクロ流体デバイスの熱測定技術

作製とAcoustofluidicのデバイスの動作は、粒子のフォーカシングSheathlessためのバルク弾性定在波を支援

完全に解決策が処理無機ナノ結晶太陽電池素子の作製

眼内レンズからの前方散乱光および後方散乱光を定量的に評価するための走査型光散乱プロファイラ（SLPS）に基づく方法論

誘電泳動の流れ支援: 高性能低温硬化型ナノワイヤデバイス作製のための低コストの方法

大型単層長方形 SnSe フレークの大気圧作製

生体の屈折率-屈折率整合素子の作製

PMMAおよびCOPマイクロ流体デバイスの作製のための溶剤接着

要約

さらに動画を探す

この動画の章

シリコン直接ウェハー接合による均一ナノスケールキャビティの作製

作製とマイクロ流体光力学発振器のテスト

クーロン不純物を走査トンネル顕微鏡研究のためのゲート - 調整可能なグラフェンデバイスの作製

分析マイクロ流体デバイスの熱測定技術

作製とAcoustofluidicのデバイスの動作は、粒子のフォーカシングSheathlessためのバルク弾性定在波を支援

完全に解決策が処理無機ナノ結晶太陽電池素子の作製

眼内レンズからの前方散乱光および後方散乱光を定量的に評価するための走査型光散乱プロファイラ（SLPS）に基づく方法論

誘電泳動の流れ支援: 高性能低温硬化型ナノワイヤ デバイス作製のための低コストの方法

大型単層長方形 SnSe フレークの大気圧作製

生体の屈折率-屈折率整合素子の作製

誘電泳動の流れ支援: 高性能低温硬化型ナノワイヤデバイス作製のための低コストの方法