Meiji University

3 ARTICLES PUBLISHED IN JoVE

Behavior

fMRI Validation of fNIRS Measurements During a Naturalistic Task

J. Adam Noah¹, Yumie Ono², Yasunori Nomoto², Sotaro Shimada², Atsumichi Tachibana³, Xian Zhang¹, Shaw Bronner¹, Joy Hirsch^1,5

¹Department of Psychiatry, Yale School of Medicine, ²Department of Electronics and Bioinformatics, Meiji University, ³Department of Histology and Neurobiology, Dokkyo Medical University School of Medicine, ⁴ADAM Center, Department of Physical Therapy, Movement and Rehabilitation Sciences, Northeastern University, ⁵Department of Neurobiology, Yale School of Medicine

We present a method to compare functional brain activity recorded during a naturalistic task using fNIRS with activity recorded during fMRI.

Behavior

Testing Animal Anxiety in Rats: Effects of Open Arm Ledges and Closed Arm Wall Transparency in Elevated Plus Maze Test

Yasuyuki Horii¹, Iain McTaggart², Maiko Kawaguchi¹

¹Laboratory of Animal Behavior and Environmental Science, School of Agriculture, Meiji University, ²Laboratory of Agri-environmental Science, School of Agriculture, Meiji University

Rats' anxiety-like behavior tested by the elevated plus maze varies highly depending on the testing environment. Here we show how apparatus settings, especially arm structure, affect behavior on the maze, and we explain a reliable protocol to detect effects of anxiolytic drugs. This report will contribute to anxiety research.

Biology

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds

Keisuke Seta¹, Daiki Shinozaki^1,2,3, Kohki Yoshimoto¹

¹Department of Life Sciences, School of Agriculture, Meiji University, ²Organization for the Strategic Coordination of Research and Intellectual Properties, Meiji University, ³Plant Functional Biotechnology, Agro-Biotechnology Research Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo

This protocol describes the preparation of intact samples of the endosperm cell layer in Arabidopsis thaliana seeds. The method requires only common laboratory equipment, such as an injection needle and precision forceps, and enables high-resolution fluorescent live-cell imaging of endosperm cells in both developing and mature seeds.