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Table of Contents

Archive: Issue 170, April 2021

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Behavior(5)
Biochemistry(7)
Bioengineering(11)
Biology(22)
Cancer Research(7)
Chemistry(1)
Developmental Biology(8)
Engineering(2)
Environment(2)
Genetics(2)
Immunology and Infection(8)
Medicine(13)
Neuroscience(10)
Calcium Imaging in Freely Behaving <em>Caenorhabditis elegans</em> with Well-Controlled, Nonlocalized Vibration

Calcium Imaging in Freely Behaving Caenorhabditis elegans with Well-Controlled, Nonlocalized Vibration

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Journal (Behavior)

Evaluation of the Cognitive Performance of Hypertensive Patients with Silent Cerebrovascular Lesions

Evaluation of the Cognitive Performance of Hypertensive Patients with Silent Cerebrovascular Lesions

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Journal (Behavior)

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Methods Collections

The Three-Chamber Choice Behavioral Task using Zebrafish as a Model System

The Three-Chamber Choice Behavioral Task using Zebrafish as a Model System

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Journal (Behavior)

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Methods Collections

Using Enclosed Y-Mazes to Assess Chemosensory Behavior in Reptiles

Using Enclosed Y-Mazes to Assess Chemosensory Behavior in Reptiles

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Journal (Behavior)

Comparative Analysis of Experimental Methods to Quantify Animal Activity in <em>Caenorhabditis elegans</em> Models of Mitochondrial Disease

Comparative Analysis of Experimental Methods to Quantify Animal Activity in Caenorhabditis elegans Models of Mitochondrial Disease

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Journal (Behavior)

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Methods Collections

Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

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Journal (Biochemistry)

Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy

Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy

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Journal (Biochemistry)

<sup>15</sup>N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

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Journal (Biochemistry)

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Methods Collections

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

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Journal (Biochemistry)

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Methods Collections

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast <em>Saccharomyces cerevisiae</em>

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae

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Journal (Biochemistry)

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Methods Collections

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

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Journal (Biochemistry)

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Methods Collections

Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block

Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block

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Journal (Biochemistry)

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Methods Collections

Continuous Measurement of Biological Noise in <em>Escherichia Coli</em> Using Time-lapse Microscopy

Continuous Measurement of Biological Noise in Escherichia Coli Using Time-lapse Microscopy

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Journal (Bioengineering)

Modulating Shape of Polyester Based Polymersomes using Osmotic Pressure

Modulating Shape of Polyester Based Polymersomes using Osmotic Pressure

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Journal (Bioengineering)

3D Printing of <em>In Vitro</em> Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

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Journal (Bioengineering)

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

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Journal (Bioengineering)

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Methods Collections

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

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Journal (Bioengineering)

Electric-Field-Induced Neural Precursor Cell Differentiation in Microfluidic Devices

Electric-Field-Induced Neural Precursor Cell Differentiation in Microfluidic Devices

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Journal (Bioengineering)

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Methods Collections

Microfluidic Model to Mimic Initial Event of Neovascularization

Microfluidic Model to Mimic Initial Event of Neovascularization

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Journal (Bioengineering)

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Methods Collections

Studying Cavitation Enhanced Therapy

Studying Cavitation Enhanced Therapy

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Journal (Bioengineering)

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Methods Collections

Targeting the Rat's Small Bowel: Long-Term Infusion into the Superior Mesenteric Artery

Targeting the Rat's Small Bowel: Long-Term Infusion into the Superior Mesenteric Artery

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Journal (Bioengineering)

Automation of Bio-Atomic Force Microscope Measurements on Hundreds of <em>C. albicans</em> Cells

Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells

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Journal (Bioengineering)

Earthworm, Lumbricus Terrestris: A Novel Microinjection Vasculature In vivo Invertebrate Model

Earthworm, Lumbricus Terrestris: A Novel Microinjection Vasculature In vivo Invertebrate Model

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Journal (Bioengineering)

Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning

Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning

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Journal (Biology)

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

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Journal (Biology)

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Methods Collections

Analysis of Motility Patterns of <em>Stentor</em> During and After Oral Apparatus Regeneration Using Cell Tracking

Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell Tracking

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Journal (Biology)

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

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Journal (Biology)

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Methods Collections

A Mouse Model of Lumbar Spine Instability

A Mouse Model of Lumbar Spine Instability

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Journal (Biology)

Size Determination and Phenotypic Analysis of Urinary Extracellular Vesicles using Flow Cytometry

Size Determination and Phenotypic Analysis of Urinary Extracellular Vesicles using Flow Cytometry

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Journal (Biology)

Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission

Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission

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Journal (Biology)

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Methods Collections

Application of Ultrasound and Shear Wave Elastography Imaging in a Rat Model of NAFLD/NASH

Application of Ultrasound and Shear Wave Elastography Imaging in a Rat Model of NAFLD/NASH

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Journal (Biology)

Isolation and Characterization of Human Adipocyte-Derived Extracellular Vesicles using Filtration and Ultracentrifugation

Isolation and Characterization of Human Adipocyte-Derived Extracellular Vesicles using Filtration and Ultracentrifugation

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Journal (Biology)

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Methods Collections

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

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Journal (Biology)

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Methods Collections

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

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Journal (Biology)

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Methods Collections

Isolation of Human Primary Valve Cells for In vitro Disease Modeling

Isolation of Human Primary Valve Cells for In vitro Disease Modeling

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Journal (Biology)

Generation of Human Kidney Tubuloids from Tissue and Urine

Generation of Human Kidney Tubuloids from Tissue and Urine

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Journal (Biology)

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Methods Collections

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

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Journal (Biology)

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Methods Collections

Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity

Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity

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Journal (Biology)

Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices

Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices

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Journal (Biology)

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Methods Collections

Chemical Dimerization-Induced Protein Condensates on Telomeres

Chemical Dimerization-Induced Protein Condensates on Telomeres

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Journal (Biology)

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Methods Collections

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

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Journal (Biology)

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control

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Journal (Biology)

Partial Hepatectomy in Adult Zebrafish

Partial Hepatectomy in Adult Zebrafish

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Journal (Biology)

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model

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Journal (Biology)

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Methods Collections

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

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Journal (Biology)

Medium-Throughput Drug- and Radiotherapy Screening Assay using Patient-Derived Organoids

Medium-Throughput Drug- and Radiotherapy Screening Assay using Patient-Derived Organoids

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Journal (Cancer Research)

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Methods Collections

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

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Journal (Cancer Research)

Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle

Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle

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Journal (Cancer Research)

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Methods Collections

Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System

Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System

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Journal (Cancer Research)

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Journal (Cancer Research)

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Methods Collections

Creation and Maintenance of a Living Biobank - How We Do It

Creation and Maintenance of a Living Biobank - How We Do It

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Journal (Cancer Research)

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Methods Collections

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

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Journal (Cancer Research)

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Methods Collections

Crystallization of Proteins on Chip by Microdialysis for <i>In Situ</i> X-ray Diffraction Studies

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies

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Journal (Chemistry)

Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish

Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish

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Journal (Developmental Biology)

Dissection, Immunohistochemistry and Mounting of Larval and Adult <em>Drosophila</em> Brains for Optic Lobe Visualization

Dissection, Immunohistochemistry and Mounting of Larval and Adult Drosophila Brains for Optic Lobe Visualization

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Journal (Developmental Biology)

Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish

Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish

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Journal (Developmental Biology)

A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells

A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells

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Journal (Developmental Biology)

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Methods Collections

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

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Journal (Developmental Biology)

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Methods Collections

Visualizing the Effects of Oxidative Damage on Drosophila Egg Chambers using Live Imaging

Visualizing the Effects of Oxidative Damage on Drosophila Egg Chambers using Live Imaging

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Journal (Developmental Biology)

Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells

Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells

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Journal (Developmental Biology)

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Methods Collections

Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish

Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish

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Journal (Developmental Biology)

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Methods Collections

A Versatile Kit Based on Digital Microfluidics Droplet Actuation for Science Education

A Versatile Kit Based on Digital Microfluidics Droplet Actuation for Science Education

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Journal (Engineering)

Micromechanical Tension Testing of Additively Manufactured 17-4 PH Stainless Steel Specimens

Micromechanical Tension Testing of Additively Manufactured 17-4 PH Stainless Steel Specimens

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Journal (Engineering)

A Field Primer for Monitoring Benthic Ecosystems Using Structure-From-Motion Photogrammetry

A Field Primer for Monitoring Benthic Ecosystems Using Structure-From-Motion Photogrammetry

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Journal (Environment)

Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

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Journal (Environment)

Using the GAL4-UAS System for Functional Genetics in <em>Anopheles gambiae</em>

Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

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Journal (Genetics)

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Methods Collections

Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders

Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders

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Journal (Genetics)

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Methods Collections

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

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Journal (Immunology and Infection)

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Methods Collections

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation

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Journal (Immunology and Infection)

Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic <em>Escherichia Coli</em>

Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic Escherichia Coli

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Journal (Immunology and Infection)

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Methods Collections

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

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Journal (Immunology and Infection)

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

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Journal (Immunology and Infection)

Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices

Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices

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Journal (Immunology and Infection)

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Methods Collections

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

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Journal (Immunology and Infection)

Tools for the Real-Time Assessment of a <em>Pseudomonas aeruginosa</em> Infection Model

Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model

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Journal (Immunology and Infection)

Quantification of Absolute Myocardial Flow and Resistance by Continuous Thermodilution in Patients with Ischemia and Nonobstructive Coronary Artery Disease (INOCA)

Quantification of Absolute Myocardial Flow and Resistance by Continuous Thermodilution in Patients with Ischemia and Nonobstructive Coronary Artery Disease (INOCA)

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Journal (Medicine)

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Methods Collections

Psychophysical Tracking Method to Assess Taste Detection Thresholds in Children, Adolescents, and Adults: The Taste Detection Threshold (TDT) Test

Psychophysical Tracking Method to Assess Taste Detection Thresholds in Children, Adolescents, and Adults: The Taste Detection Threshold (TDT) Test

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Journal (Medicine)

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Methods Collections

Directed Induction of Retinal Organoids from Human Pluripotent Stem Cells

Directed Induction of Retinal Organoids from Human Pluripotent Stem Cells

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Journal (Medicine)

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Methods Collections

Piezo-ICSI for Human Oocytes

Piezo-ICSI for Human Oocytes

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Journal (Medicine)

Laser Doppler Perfusion Imaging in the Mouse Hindlimb

Laser Doppler Perfusion Imaging in the Mouse Hindlimb

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Journal (Medicine)

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Methods Collections

Direct Drug Delivery to Kidney via the Renal Artery

Direct Drug Delivery to Kidney via the Renal Artery

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Journal (Medicine)

Post-Myocardial Infarction Heart Failure in Closed-chest Coronary Occlusion/Reperfusion Model in G&#246;ttingen Minipigs and Landrace Pigs

Post-Myocardial Infarction Heart Failure in Closed-chest Coronary Occlusion/Reperfusion Model in Göttingen Minipigs and Landrace Pigs

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Journal (Medicine)

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Methods Collections

Creation of Two Saccular Elastase-Digested Aneurysms with Different Hemodynamics in One Rabbit

Creation of Two Saccular Elastase-Digested Aneurysms with Different Hemodynamics in One Rabbit

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Journal (Medicine)

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Methods Collections

3D Imaging of PDL Collagen Fibers during Orthodontic Tooth Movement in Mandibular Murine Model

3D Imaging of PDL Collagen Fibers during Orthodontic Tooth Movement in Mandibular Murine Model

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Journal (Medicine)

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Methods Collections

TBase - an Integrated Electronic Health Record and Research Database for Kidney Transplant Recipients

TBase - an Integrated Electronic Health Record and Research Database for Kidney Transplant Recipients

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Journal (Medicine)

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Methods Collections

Retinal Organoid Induction System for Derivation of 3D Retinal Tissues from Human Pluripotent Stem Cells

Retinal Organoid Induction System for Derivation of 3D Retinal Tissues from Human Pluripotent Stem Cells

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Journal (Medicine)

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Methods Collections

Digital Home-Monitoring of Patients after Kidney Transplantation: The MACCS Platform

Digital Home-Monitoring of Patients after Kidney Transplantation: The MACCS Platform

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Journal (Medicine)

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Methods Collections

Surfactant Depletion Combined with Injurious Ventilation Results in a Reproducible Model of the Acute Respiratory Distress Syndrome (ARDS)

Surfactant Depletion Combined with Injurious Ventilation Results in a Reproducible Model of the Acute Respiratory Distress Syndrome (ARDS)

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Journal (Medicine)

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Methods Collections

Objectively Assessing Sports Concussion Utilizing Visual Evoked Potentials

Objectively Assessing Sports Concussion Utilizing Visual Evoked Potentials

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Journal (Neuroscience)

Preparing Adult <em>Drosophila melanogaster</em> for Whole Brain Imaging during Behavior and Stimuli Responses

Preparing Adult Drosophila melanogaster for Whole Brain Imaging during Behavior and Stimuli Responses

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Journal (Neuroscience)

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Methods Collections

Extraction and Dissection of the Domesticated Pig Brain

Extraction and Dissection of the Domesticated Pig Brain

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Journal (Neuroscience)

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

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Journal (Neuroscience)

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Methods Collections

&#181;Tongue: A Microfluidics-Based Functional Imaging Platform for the Tongue <em>In Vivo</em>

µTongue: A Microfluidics-Based Functional Imaging Platform for the Tongue In Vivo

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Journal (Neuroscience)

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Methods Collections

Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing

Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing

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Journal (Neuroscience)

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Methods Collections

Detecting Amyloid-&#946; Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease

Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease

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Journal (Neuroscience)

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Methods Collections

Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting

Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting

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Journal (Neuroscience)

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Methods Collections

Investigation of Activated Mouse Olfactory Sensory Neurons via Combined Immunostaining and <em>in situ</em> Hybridization

Investigation of Activated Mouse Olfactory Sensory Neurons via Combined Immunostaining and in situ Hybridization

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Journal (Neuroscience)

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Methods Collections

Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice

Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice

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Journal (Neuroscience)

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Methods Collections

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