Name: handheld metal detector screening for metallic foreign body ingestion in children
Uploaded: 2018-09-11T15:00:00.000+00:00
Duration: 4 min 55 s
Description: Watch this Scientific Journal Video about Handheld Metal Detector Screening for Metallic Foreign Body Ingestion in Children at JoVE.com

作者感谢李潘文凯针, 头 & 资深顾问, 儿童急诊, KK 妇女和儿童医院, 100 武吉天然路, 新加坡为行政支持与视频文章制作。在 "儿科紧急情况下金属异物摄取手持金属探测器" 许可下转载的文章;2018年8月:85 (8): 618–624;Hazwani Binte 哈姆扎王子、守夜詹姆斯、苏拉吉马尼卡姆、Sashikumar Ganapathy: 版权所有印度儿科杂志。

该议定书遵循机构人类研究伦理委员会的准则。中央机构审查委员会为这项研究提供了道德上的批准。
1. 参加者筛选
<ol><li>招募向儿科急诊科的病人提供异物摄取病史。</li>
	<li>排除呼吸窘迫的病人, 并植入了类似于速度器的设备。</li>
</ol>2. 准备病人
<ol><li>给病人穿上医院礼服。</li>
	<li>删除所有可能包含金属物体, 如衣服, 眼睛眼镜, 耳环, 挂件, 和饰物在检查之前, 以避免干扰 HHMD 筛查。</li>
	<li>把病人放在房间的中心, 远离其他潜在的金属干扰。</li>
</ol>3. 准备考官和照顾者
<ol><li>卸下所有金属配件, 如首饰和手表。</li>
	<li>请护理人员删除所有金属配件, 如首饰和手表。</li>
</ol>4. 金属探测器筛查
<ol><li>使用 HHMD (图 1) 进行筛选。</li>
	<li>通过在金属片上挥舞一个正面的视听信号, 确认 HHMD 的工作状态。</li>
	<li>对较大的孩子进行 HHMD 扫描。</li>
	<li>在婴儿的直立位置进行 HHMD 扫描, 让看护者适当地控制病人。</li>
	<li>握住病人的手, 远离身体。</li>
	<li>将病人的头部放在延伸处以暴露颈部。</li>
	<li>从下巴的水平扫描到髋关节的水平, 从颈部到骨盆覆盖解剖区域。</li>
	<li>在整个身体内以曲折的方式执行扫描, 以确保扫描每个区域。</li>
	<li>扫描前方和向后作为患者的厚度必须考虑。</li>
	<li>仔细注意在扫描期间的正面视听信号 MFBs 存在。</li>
</ol>5. 执行相关的射线照相
<ol><li>根据 MFBs HHMD 筛查的区域, 订购颈部、胸部或腹部的相关射线。</li>
	<li>如果在 HHMD 筛查时没有发现 MFB, 则继续进行所有 X 线观察 (颈部、胸部和腹部), 而对异物摄取的怀疑的临床指标很高。</li>
</ol>6. 记录测试结果
<ol><li>记录根据工作流专门创建的日志 (图 2) 中的测试结果 (图1)。</li>
	<li>通过 palpating 以下突出的骨性地标-胸骨凹槽、xiphisternum、耻骨联合和双侧髂嵴, 将正的视听 HHMD 信号与身体底层的骨性地标相关联。</li>
	<li>在进行 X 光检查之前, 在航海日志的解剖参考提纲上记录地标。</li>
</ol>

<ol>
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作者没有什么可透露的。

金属异物在儿童中摄取是一个常见的问题, 在大多数患者中, 这是保守的管理7,16。单一或重复的射线照相可以暴露敏感的儿科人群的不必要的辐射风险, 而试图本地化的摄取金属异物。一些研究已经描述了使用金属探测器在 MFB 摄取儿童, 灵敏度超过 96%, 特异性大于 80%1,7,17,<sup...

由于婴儿和儿童的自然好奇性, 通过品尝和吞咽1,2来探索物体, 异物摄取在儿童急诊室中是一个普遍存在的问题。以前的研究报告, 金属物体构成85% 的所有异物 ingestions 在他们的队列的病人3,4。本议定书的目标是评估使用手持金属探测器检测儿童摄取的金属异物的准确性和可行性。我们建议, 通过在对金属异物摄取高度怀疑的儿童的分流过程中及早引入手持金属检测器筛选协议, 被下令定位金属异物的射线照相数量可以减少了这种对无线电敏感的人群。
手持金属探测器 (HHMD) 是一种价格低廉、易于获取的辅助辅料, 可帮助加快对摄取金属异物的管理。金属探测器是一种非常低频的装置, 它有一个接收器和一个发射机, 它能检测到在其附近3的金属物体的存在所引起的电磁场的变化。如果金属物体在磁场中, 电磁场就会中断, 传感器将在设备4上设置视听报警。金属探测器检查可以在不到2分5分钟内彻底完成。因此, HHMD 筛查可以在早期的诊断儿童的分流过程中高怀疑金属异物摄取的地形定位。
硬币是最常见的吞食金属异物1,4,6,7。由于缺乏锋利的边缘和一般无毒的性质, 大多数硬币 ingestions 管理保守。一般摄取的其他类型的材料包括磁铁、纽扣电池、小玩具、花生、首饰、纽扣、骨头、种子和爆米花8、9。异物的冲击最常见发生在食管三处, 即上食管括约肌, 中食管在主动脉弓水平, 下食管括约肌10。金属异物 (MFBs) 位于食管可能是无症状的40% 患者11,12 , 其长期的撞击可能导致各种并发症, 如食管穿孔, 纵隔,气管食管瘘, 气管狭窄, 主动脉食管瘘1,13,14,15。其他部位的异物嵌塞包括幽门, 十二指肠和 ileo-盲肠交界10。
执行颈部、胸部和腹部的射线照相来诊断疑似异物摄取是5紧急部门的普遍做法。在所有被摄取的异物中, 只有10% 是无线电不透明的10。放射检查是耗时的, 并有危险的辐射暴露在儿童。精确的地形定位的摄取金属物体是非常重要的指导治疗。在分流过程早期引入 HHMD 筛查可以通过地形对摄取的金属异物进行定位, 帮助减轻放射摄影的外部排列。
本研究评价了 HHMD 在系统地形学中定位 MFBs 的准确性, 确定了 HHMD 在当地儿科人群中的有效性和可行性。我们还研究了这种辐射免费筛查工具是如何良好的病人和家长接受。在可能和2016年7月之间, 所有连续的患者递交到儿科急诊科以外国身体摄取的历史被包括在研究中。知情同意是从父母或法定监护人那里获得的。使用 HHMD 对指称异物摄取的患者进行系统扫描。对摄取金属异物进行鉴定和定位的金标准是对宫颈区、胸腔和腹部的普通影像。为便于进行研究, 创建了一个标准化的工作流 (图 1)。根据工作流专门创建的日志簿用于收集初始数据, 随后将其转录到电子表格 (图 2)。结果部分中的数据被描述为按百分比显示的事例数。

<table><tbody><tr><td>Hand held metal detector</td><td>Garrett Super Scanner V</td><td>NA</td><td>NA</td></tr></tbody></table>

handheld metal detector screening for metallic foreign body ingestion in children

硬币是儿童中最常见的摄取金属异物。本议定书的目标是评估使用手持金属探测器检测儿童摄取的金属异物的准确性和可行性。我们建议, 通过引进手持金属探测器筛查早期的儿童的分流过程中, 高怀疑金属异物摄取, 可以减少的射线照相的数量, 以本地化金属异物可在此无线电敏感的人口。这项研究的议定书要求对参加者进行检查, 以了解外国身体摄取和排除呼吸窘迫或金属植入物患者的病史。病人改变了医院礼服和物品, 可能含有金属如眼镜, 耳环, 挂件, 和装饰品被删除。病人被安置在房间的中心远离其他金属干扰。手持式金属探测器的工作状态首先通过引出一个正面的视听信号来确认。然后, 筛查是在一个直立的位置, 头部延伸, 以揭露颈部, 从下巴水平到髋关节水平, 以前方和向后的曲折方式覆盖解剖区域从颈部到骨盆。在扫描时仔细注意到有金属异物存在的正面视听信号。有关的射线照相根据检测到的金属探测器筛查的区域排序。手持金属探测器能够精确地识别我们研究中摄取的金属异物中的所有硬币。手持式金属探测器不能始终如一地检测非硬币金属异物。该协议说明手持金属探测器在识别和定位硬币和硬币等金属异物方面的准确性。

研究人员为这项观察性研究招募了36名病人。这些病人已向儿科急诊科提交了一份外国身体摄取的病史。其中28例患者有金属异物 ingestions (图 3)。其余的8例患者摄取了非金属异物, 如鱼骨、沙爹棒和塑料纽扣。硬币是最常见的异物摄取类型 (表 1), 其中包括30.5% 的病人。其他最常见的异物, 被摄取包括鱼骨, 玩具零件, 纽扣电池, 金属螺丝。...

Watch this Scientific Journal Video about Handheld Metal Detector Screening for Metallic Foreign Body Ingestion in Children at JoVE.com

Handheld Metal Detector Screening for Metallic Foreign Body Ingestion in Children

在这里, 我们提出了一个使用手持金属探测器的协议, 用来筛查那些向儿科急诊医学系提供异物摄取病史的儿童的摄入金属异物。

金属异物在儿童体内摄取的手持金属探测器筛选

Coins are the most common ingested metallic foreign bodies among children. The goal of this protocol is to assess the accuracy and feasibility of using a ...

handheld-metal-detector-screening-for-metallic-foreign-body-ingestion

Universidad de Cartagena UDEC

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JoVE Journal

Medicine

10.6K 次观看.  KK women's and Children's Hospital. 在这里, 我们提出了一个使用手持金属探测器的协议, 用来筛查那些向儿科急诊医学系提供异物摄取病史的儿童的摄入金属异物。

视频: Handheld Metal Detector Screening for Metallic Foreign Body Ingestion in Children

Bacterial Detection &amp; Identification Using Electrochemical Sensors

Electrochemical sensors are widely used for rapid and accurate measurement of blood glucose and can be adapted for detection of a wide variety of analytes. Electrochemical sensors operate by transducing a biological recognition event into a useful electrical signal. Signal transduction occurs by coupling the activity of a redox enzyme to an amperometric electrode. Sensor specificity is either an inherent characteristic of the enzyme, glucose oxidase in the case of a glucose sensor, or a product of linkage between the enzyme and an antibody or probe.
Here, we describe an electrochemical sensor assay method to directly detect and identify bacteria. In every case, the probes described here are DNA oligonucleotides. This method is based on sandwich hybridization of capture and detector probes with target ribosomal RNA (rRNA). The capture probe is anchored to the sensor surface, while the detector probe is linked to horseradish peroxidase (HRP). When a substrate such as 3,3',5,5'-tetramethylbenzidine (TMB) is added to an electrode with capture-target-detector complexes bound to its surface, the substrate is oxidized by HRP and reduced by the working electrode. This redox cycle results in shuttling of electrons by the substrate from the electrode to HRP, producing current flow in the electrode.

Bacterial Detection & Identification Using Electrochemical Sensors

We describe an electrochemical sensor assay method for rapid bacterial detection and identification. The assay involves a sensor array functionalized with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. Sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase-linked DNA oligonucleotide detector probe produces a measurable amperometric current.

使用电化学传感器的细菌检测和鉴定

Electrochemical  sensors are widely used for rapid and accurate measurement of blood glucose and  can be adapted for detection of a wide variety of ...

科研

生物工程

Protocol for the Evaluation of MRI Artifacts Caused by Metal Implants to Assess the Suitability of Implants and the Vulnerability of Pulse Sequences

As the number of magnetic resonance imaging (MRI) scanners and patients with medical implants is constantly growing, radiologists increasingly encounter metallic implant-related artifacts in MRI, resulting in reduced image quality. Therefore, the MRI suitability of implants in terms of artifact volume, as well as the development of pulse sequences to reduce image artifacts, are becoming more and more important. Here, we present a comprehensive protocol which allows for a standardized evaluation of the artifact volume of implants on MRI. Furthermore, this protocol can be used to analyze the vulnerability of different pulse sequences to artifacts. The proposed protocol can be applied to T1- and T2-weighted images with or without fat-suppression and all passive implants. Furthermore, the procedure enables the separate and three-dimensional identification of signal loss and pile-up artifacts. As previous investigations differed greatly in evaluation methods, the comparability of their results was limited. Thus, standardized measurements of MRI artifact volumes are necessary to provide better comparability. This may improve the development of the MRI suitability of implants and better pulse sequences to finally improve patient care.

We describe a standardized method to evaluate magnetic resonance imaging artifacts caused by implants to estimate the suitability of the implants for magnetic resonance imaging and/or the vulnerability of different pulse sequences to metallic artifacts simultaneously.

评估植入物所引起的 MRI 伪影和脉冲序列易损性评估的协议

As the number of magnetic resonance imaging (MRI) scanners and patients with medical implants is constantly growing, radiologists increasingly encounter ...

工程学

Making MR Imaging Child's Play - Pediatric Neuroimaging Protocol, Guidelines and Procedure

Within the last decade there has been an increase in the use of structural and functional magnetic resonance imaging (fMRI) to investigate the neural basis of human perception, cognition and behavior 1, 2. Moreover, this non-invasive imaging method has grown into a tool for clinicians and researchers to explore typical and atypical brain development. Although advances in neuroimaging tools and techniques are apparent, (f)MRI in young pediatric populations remains relatively infrequent 2. Practical as well as technical challenges when imaging children present clinicians and research teams with a unique set of problems 3, 2. To name just a few, the child participants are challenged by a need for motivation, alertness and cooperation. Anxiety may be an additional factor to be addressed. Researchers or clinicians need to consider time constraints, movement restriction, scanner background noise and unfamiliarity with the MR scanner environment2,4-10. A progressive use of functional and structural neuroimaging in younger age groups, however, could further add to our understanding of brain development. As an example, several research groups are currently working towards early detection of developmental disorders, potentially even before children present associated behavioral characteristics e.g.11. Various strategies and techniques have been reported as a means to ensure comfort and cooperation of young children during neuroimaging sessions. Play therapy 12, behavioral approaches 13, 14,15, 16-18 and simulation 19, the use of mock scanner areas 20,21, basic relaxation 22 and a combination of these techniques 23 have all been shown to improve the participant's compliance and thus MRI data quality. Even more importantly, these strategies have proven to increase the comfort of families and children involved 12. One of the main advances of such techniques for the clinical practice is the possibility of avoiding sedation or general anesthesia (GA) as a way to manage children's compliance during MR imaging sessions 19,20. In the current video report, we present a pediatric neuroimaging protocol with guidelines and procedures that have proven to be successful to date in young children.

Despite an increase in the use of structural and functional magnetic resonance imaging (fMRI) in humans, the study of young pediatric populations remains a challenge. We present a hands-on, step-by-step video protocol including guidelines for clinicians and researchers intending to perform (f)MRI in young children.

使磁共振成像儿戏 - 小儿神经影像学的协议，指引及程序

Within the last decade there has been an increase in the use of structural and functional magnetic resonance imaging (fMRI) to investigate the neural ...

生物学

Ferromagnetic Bare Metal Stent for Endothelial Cell Capture and Retention

Rapid endothelialization of cardiovascular stents is needed to reduce stent thrombosis and to avoid anti-platelet therapy which can reduce bleeding risk. The feasibility of using magnetic forces to capture and retain endothelial outgrowth cells (EOC) labeled with super paramagnetic iron oxide nanoparticles (SPION) has been shown previously. But this technique requires the development of a mechanically functional stent from a magnetic and biocompatible material followed by in-vitro and in-vivo testing to prove rapid endothelialization. We developed a weakly ferromagnetic stent from 2205 duplex stainless steel using computer aided design (CAD) and its design was further refined using finite element analysis (FEA). The final design of the stent exhibited a principal strain below the fracture limit of the material during mechanical crimping and expansion. One hundred stents were manufactured and a subset of them was used for mechanical testing, retained magnetic field measurements, in-vitro cell capture studies, and in-vivo implantation studies. Ten stents were tested for deployment to verify if they sustained crimping and expansion cycle without failure. Another 10 stents were magnetized using a strong neodymium magnet and their retained magnetic field was measured. The stents showed that the retained magnetism was sufficient to capture SPION-labeled EOC in our in-vitro studies. SPION-labeled EOC capture and retention was verified in large animal models by implanting 1 magnetized stent and 1 non-magnetized control stent in each of 4 pigs. The stented arteries were explanted after 7 days and analyzed histologically. The weakly magnetic stents developed in this study were capable of attracting and retaining SPION-labeled endothelial cells which can promote rapid healing.

Our goals were to design, manufacture and test ferromagnetic stents for endothelial cell capture. Ten stents were tested for fracture and 10 more stents were tested for retained magnetism. Finally, 10 stents were tested in-vitro and 8 more stents were implanted in 4 pigs to show cell capture and retention.

铁磁金属裸支架对血管内皮细胞捕获和保留

Rapid endothelialization of cardiovascular stents is needed to reduce stent thrombosis and to avoid anti-platelet therapy which can reduce bleeding risk. ...

Preparation and Application of a New Bacterial Biosensor for the Presumptive Detection of Gunshot Residue

MicRoboCop is a biosensor that has been designed for a unique application in forensic chemistry. MicRoboCop is a system made up of three devices that, when used together, can indicate the presence of gunshot residue (GSR) by producing a fluorescence signal in the presence of three key analytes (antimony, lead, and organic components of GSR). The protocol describes the synthesis of the biosensors using Escherichia coli (E. coli), and the analytical chemistry methods used to evaluate the selectivity and sensitivity of the sensors. The functioning of the system is demonstrated by using GSR collected from the inside of a spent cartridge casing. Once prepared, the biosensors can be stored until needed and can be used as a test for these key analytes. A positive response from all three analytes provides a presumptive positive test for GSR, while each individual device has applications for detecting the analytes in other samples (e.g., a detector for lead contamination in drinking water). The main limitation of the system is the time required for a positive signal; future work may involve studying different organisms to optimize the response time.

A protocol is presented using synthetic biology techniques to synthesize a set of bacterial biosensors for the analysis of gunshot residue, and to test the functioning of the devices for their intended use using fluorescence spectroscopy.

一种用于射击残留量推定检测的新型细菌生物传感器的研制与应用

MicRoboCop is a biosensor that has been designed for a unique application in forensic chemistry. MicRoboCop is a system made up of three devices that, ...

化学

Hellgrammite as a Non-Conventional Biomonitor for Surface Water and Environmental Health Assessment

The larvae of dobsonflies from the genus Corydalus, commonly known as Hellgrammites, are characterized by their notable size, extensive range of occurrence, and extended period of immaturity, which can last up to one year. Hellgrammites are clearly known to exhibit sensitivity to pollution and habitat structure impacts. Given these unique features, using Corydalus texanus larvae is highly suitable as reliable biomonitoring agents to assess the ecological integrity of aquatic ecosystems. This protocol aims to provide the necessary tools for C. texanus assessment and demonstrate their efficacy through a case study. Research findings have practical implications, indicating that C. texanus larvae exhibit early-warning responses to mining pollution, bioaccumulating high amounts of heavy metals such as Zn, Fe, and Al. The presence or absence of C. texanus populations may serve as a helpful indicator for identifying potential issues related to ecosystem health. The unconventional approach has shown early warnings of pollution in mining-impacted sites, highlighting the need for timely action to protect the environment. Given their unique traits, the use of C. texanus larvae is highly suggested as a reliable non-conventional bioindicator.

This protocol describes the use of biomarkers for the early detection of deleterious impacts in aquatic ecosystems. The biomarkers are closely related to sentinel traits, and their changes aid in detecting early-warning damages.

Hellgrammite 作为地表水和环境健康评估的非常规生物监测器

The larvae of dobsonflies from the genus Corydalus, commonly known as Hellgrammites, are characterized by their notable size, extensive range of ...

环境科学

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

Contamination of enzymes with metals leached from immobilized metal affinity chromatography (IMAC) columns poses a major concern for enzymologists, as many of the common di-and trivalent cations used in IMAC resins have an inhibitory effect on enzymes. However, the extent of metal leaching and the impact of various eluting and reducing reagents are poorly understood in large part due to the absence of simple and practical transition metal quantification protocols that use equipment typically available in biochemistry labs. To address this problem, we have developed a protocol to quickly quantify the amount of metal contamination in samples prepared using IMAC as a purification step. The method uses hydroxynaphthol blue (HNB) as a colorimetric indicator for metal cation content in a sample solution and UV-Vis spectroscopy as a means to quantify the amount of metal present, into the nanomolar range, based on the change in the HNB spectrum at 647 nm. While metal content in a solution has historically been determined using atomic absorption spectroscopy or inductively coupled plasma techniques, these methods require specialized equipment and training outside the scope of a typical biochemistry laboratory. The method proposed here provides a simple and fast way for biochemists to determine the metal content of samples using existing equipment and knowledge without sacrificing accuracy.

We present an assay for easy quantification of metals introduced to samples prepared using immobilized metal affinity chromatography. The method uses hydroxynaphthol blue as the colorimetric metal indicator and a UV-Vis spectrophotometer as the detector.

金属浸出在固定金属亲和力色谱中的定量

Contamination of enzymes with metals leached from immobilized metal affinity chromatography (IMAC) columns poses a major concern for enzymologists, as ...

Magnetic Levitation Coupled with Portable Imaging and Analysis for Disease Diagnostics

Currently, many clinical diagnostic procedures are complex, costly, inefficient, and inaccessible to a large population in the world. The requirements for specialized equipment and trained personnel require that many diagnostic tests be performed at remote, centralized clinical laboratories. Magnetic levitation is a simple yet powerful technique and can be applied to levitate cells, which are suspended in a paramagnetic solution and placed in a magnetic field, at a position determined by equilibrium between a magnetic force and a buoyancy force. Here, we present a versatile platform technology designed for point-of-care diagnostics which uses magnetic levitation coupled to microscopic imaging and automated analysis to determine the density distribution of a patient's cells as a useful diagnostic indicator. We present two platforms operating on this principle: (i) a smartphone-compatible version of the technology, where the built-in smartphone camera is used to image cells in the magnetic field and a smartphone application processes the images and to measures the density distribution of the cells and (ii) a self-contained version where a camera board is used to capture images and an embedded processing unit with attached thin-film-transistor (TFT) screen measures and displays the results. Demonstrated applications include: (i) measuring the altered distribution of a cell population with a disease phenotype compared to a healthy phenotype, which is applied to sickle cell disease diagnosis, and (ii) separation of different cell types based on their characteristic densities, which is applied to separate white blood cells from red blood cells for white blood cell cytometry. These applications, as well as future extensions of the essential density-based measurements enabled by this portable, user-friendly platform technology, will significantly enhance disease diagnostic capabilities at the point of care.

We present a magnetic levitation technique coupled with automated imaging and analysis in both a smartphone-compatible device and a device with embedded imaging and processing. This is applied to measure the density distribution of cells with two demonstrated biomedical applications: sickle cell disease diagnosis and separating white and red blood cells.

磁悬浮加上便携式成像与分析诊断疾病

Currently, many clinical diagnostic procedures are complex, costly, inefficient, and inaccessible to a large population in the world. The requirements for ...

Magnetic Stirrer Method for the Detection of Trichinella Larvae in Muscle Samples

Trichinellosis is a debilitating disease in humans and is caused by the consumption of raw or undercooked meat of animals infected with the nematode larvae of the genus Trichinella. The most important sources of human infections worldwide are game meat and pork or pork products. In many countries, the prevention of human trichinellosis is based on the identification of infected animals by means of the artificial digestion of muscle samples from susceptible animal carcasses.
There are several methods based on the digestion of meat but the magnetic stirrer method is considered the gold standard. This method allows the detection of Trichinella larvae by microscopy after the enzymatic digestion of muscle samples and subsequent filtration and sedimentation steps. Although this method does not require special and expensive equipment, internal controls cannot be used. Therefore, stringent quality management should be applied throughout the test. The aim of the present work is to provide detailed handling instructions and critical control points of the method to analysts, based on the experience of the European Union Reference Laboratory for Parasites and the National Reference Laboratory of Germany for Trichinella.

Magnetic Stirrer Method for the Detection of Trichinella Larvae in Muscle Samples

The prevention of human trichinellosis in many countries worldwide is based on the laboratory examination of muscle samples from susceptible animals by methods of digestion, of which the magnetic stirrer method is considered the gold standard.

对于检测磁力搅拌器方法<em&gt;旋毛虫</em&gt;幼虫肌肉样品

Trichinellosis is a debilitating disease in humans and is caused by the consumption of raw or undercooked meat of animals infected with the nematode ...

免疫与感染

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Enterohemorrhagic Escherichia coli O157:H7 has been linked to both waterborne and foodborne illnesses, and remains a threat despite the food- and water-screening methods used currently. While conventional bacterial detection methods, such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assays (ELISA) can specifically detect pathogenic contaminants, they require extensive sample preparation and lengthy waiting periods. In addition, these practices demand sophisticated laboratory instruments and settings, and must be executed by trained professionals. Herein, a protocol is proposed for a simpler diagnostic technique that features the unique combination of magnetic and fluorescent parameters in a nanoparticle-based platform. The proposed multiparametric magneto-fluorescent nanosensors (MFnS) can detect E. coli O157:H7 contamination with as little as 1 colony-forming unit present in solution within less than 1 h. Furthermore, the ability of MFnS to remain highly functional in complex media such as milk and lake water has been verified. Additional specificity assays were also used to demonstrate the ability of MFnS to only detect the specific target bacteria, even in the presence of similar bacterial species. The pairing of magnetic and fluorescent modalities allows for the detection and quantification of pathogen contamination in a wide range of concentrations, exhibiting its high performance in both early- and late-stage contamination detection. The effectiveness, affordability, and portability of the MFnS make them an ideal candidate for point-of-care screening for bacterial contaminants in a wide range of settings, from aquatic reservoirs to commercially packaged foods.

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

The overall goal of this protocol is to synthesize functional nanosensors for the portable, cost-effective, and rapid detection of specifically targeted pathogenic bacteria through a combination of magnetic relaxation and fluorescence emission modalities.

金属异物在儿童体内摄取的手持金属探测器筛选

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