作者没有确认。

根据机构指南, 动物被安置在南丹麦大学动物保育设施中。所有动物实验都是按照国家卫生研究院的指南进行的, 用于实验室动物的护理和使用。这是一个急性的, 非生存的手术程序。
1. 导管、电极及手术器械的制备
<ol><li>使用以下显微外科器械: 手术剪刀, 角度微剪刀, 纸巾钳, 一对 #7 和 #5 弯曲的微型钳, 微型针持有人, 和拉钩。 注意:由于这是一个急性的程序, 这些仪器不需要消毒。使用后, 清洁和擦拭的提示与70% 乙醇。</li>
	<li>将油管浸泡在70% 乙醇中, 然后在使用前用 100 U/毫升肝素冲洗0.9% 氯化钠。保持油管填充, 以避免引入气泡进入系统。</li>
	<li>将20-30 厘米长的聚乙烯 (PE)-50 油管切割成导管进行 ICP 测量。确保油管尽可能短, 以减少阻尼压力。</li>
	<li>弯曲一个无菌的24G 针的一侧到一边, 直到针打破中间。将该片与斜角连接到 PE 导管的远端, 以便插入到小弟弟小腿中。将另一半插入轮毂以连接到压力传感器。用血气盐水 (100 U/毫升) 填充系统。</li>
	<li>为了使双极聚四氟乙烯涂层电极, 切割两个125µm 银线, 以同等长度。使用一块胶带将电线连接到桌子的边缘, 并将它们串在一起。随后, 将电极连接到黑色板上。</li>
	<li>在聚四氟乙烯上做一个小切口, 用 #5 微钳将4-5 毫米长的聚四氟乙烯涂层从电极的尖端上剥开。用手术刀剪下窍门, 以达到均匀长度, 并通过弯曲刀刃钝边缘周围的两端来创建钩子。</li>
	<li>将电极贴在黑色板的边缘上, 用钩指向向上。十年代将硅胶混合在塑料板上, 将胶泡在电极周围1-2 毫米的钩子上包裹起来。</li>
	<li>在使用前允许它干燥大约5分钟 (图 1)。从另一端的较长的部分剥离聚四氟乙烯, 以允许与刺激器连接。 注意:随着在远端的钩重新做, 电极可以重复使用多次。</li>
</ol>2. 动物的制备
<ol><li>麻醉后, 将腹部、颈部和会阴下半部剃去。用70% 酒精擦洗动物, 接着是聚维酮碘三倍。把老鼠放在一个仰卧位置的加热手术垫上。应用兽医眼膏, 将麻醉转到鼻锥, 2.5% 异氟醚和0.8 升/分钟氧作为载体。 注意:调整异氟醚和氧气的水平, 以达到可接受的麻醉水平。</li>
</ol>3. 术前准备
<ol><li>在操作显微镜下完成整个手术过程: 从3.15X 到20X 的放大倍数是足够的。在整个手术中使用手套和保持一个干净的环境。把老鼠放在褶皱上。</li>
</ol>4. ICP 测量用 Ischiocavernosus 肌清扫术
<ol><li>使用手术刀, 直剪刀和 #7 弯曲的微型钳, 使1厘米垂直皮肤切口5毫米侧向中线开始在基部的小弟弟和向下延伸 (图 2A)。使用 Q 尖和仔细分开的筋膜侧向阴囊 (图 2B)。解剖筋膜后, 附上拉钩和触及与棉花倾斜交换找到坐骨股骨 (图 2C)。</li>
	<li>解剖通过脂肪组织内侧的这一点, 直到 ischiocavernosus 肌肉可视化 (图 3A)。使用一对 #7 弯曲的微型钳和纵向分开的肌肉。膜将显示为明亮的白色结构 (图 3B)。使用微钳和微剪刀, 充分暴露膜, 以查看其课程 (图 3C)。</li>
	<li>校准系统设置后 , 通过会的皮肤插入油管 , 确保它与小弟弟小腿平行 (图 4) 。保持线到位, 使切口湿润生理盐水。</li>
</ol>5. 用于刺激的 CN 解剖
<ol><li>做一个2厘米低, 中线腹部切口通过皮肤使用, 首先, 手术刀, 然后一对直剪刀和微钳。创建一个匹配切口通过筋膜沿 linea 和底层肌肉组织暴露膀胱和前列腺。</li>
	<li>使用拉钩达到良好的曝光率。使用棉尖拭子分离前列腺从脂肪组织, 以获得清晰可视化的主要骨盆神经节 (加仑) 和 CN, 运行在侧方面的前列腺 (图 5)。</li>
	<li>在对加仑和 CN 的可视化后, 小心地切开上面2-5 毫米神经的筋膜, 用角度微剪刀 (图 6a)。使用 #5 微型钳, 传播神经的每一侧和它下面的组织, 以释放4毫米长的部分 (图 6b), 并在神经下滑动9-0 缝合 (图 6c)。</li>
	<li>在缝合 (图 7a) 的帮助下轻微地提升神经, 以便在神经周围放置双极电极的钩子 (图 7b)。让助手将双组分硅胶与胰岛素针的尖端混合在一起5秒. 干神经, 将胶水涂在钩子和神经周围的区域 (图 7c, d)。在电极上稍微拉上一点, 使其神经升高大约1分钟, 让胶水干。</li>
	<li>除去拉钩, 除了右侧的拉钩, 以避免任何拉或扭的电极。用生理盐水将裸露的器官湿润, 在切口上浸泡在生理盐水中。</li>
</ol>6. ICP 测量用海绵体插管法
<ol><li>用拉钩恢复膜的可视化。确保不要将拉钩附着在 ischiocavernosus 肌肉上, 因为它会扭曲小腿。</li>
	<li>将针和血气盐水冲洗油管, 然后再将其引入膜。保持膜拉伸, 使用 #7 弯曲的微型钳一只手 (非显性), 持有的膜和其余的上覆肌肉远端到插入点。用直的微钳握住针在另一主导的手, 并确保介绍它与海绵体的过程平行 (图 8)。</li>
	<li>将针5-8 毫米推入海绵体。冲洗油管并按下小腿以测试线路 (图 9)。确保没有泄漏。用胶带将油管固定在桌子上, 以防意外拉线。删除拉钩。</li>
</ol>7. 对 CN 的刺激
<ol><li>与录音程序 (例如, 穗 2) 运行, 连续记录海绵和平均动脉压力。</li>
	<li>在刺激器上设置以下参数 (例如, SD9 草仪器, 请参见材料表), 用于 CN 刺激: 电流为1.5 毫安, 频率为16赫兹, 电压为3伏, 脉宽为5毫秒. 应用五十年代刺激以极小值 1 min在刺激之间休息。 注意:第一次刺激通常会导致响应减少 (图 10)。</li>
</ol>

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作者没有什么可透露的。

本研究的主要目的是描述一个简化的手术技术的小弟弟小腿插管的 ICP 记录和分离的 CN 为刺激。我们介绍了海绵体解剖的修改, 以简化手术, 并提供可重现性的记录, 增加的 ICP 与 CN 刺激。与1厘米垂直皮肤切口, 侧向基部的小弟弟, 用可触摸的坐骨股骨作为指导, 我们取得了良好的暴露 ischiocavernosus 肌肉和膜。此过程比文献中描述的更快 (低于15分钟), 导致最小组织中断2,<sup...

体内对实验动物勃起功能的研究始于 1863年, 埃克哈德1的开创性实验工作。刺激的骨盆神经被用来诱发更高的 ICP 的狗。在整个 20th世纪, 类似的实验性协议被用于大型动物, 如狗, 猴子, 猫和兔子。在 1989年2中, 由昆兰et 等) 首次开发出大鼠勃起功能的评价。此后, 该方法已被其他几个组34修改和更新。今天, 大鼠是最广泛使用的动物模型, 研究勃起功能障碍的病理学和评估新的治疗方案。该程序的主要步骤包括, 记录全身血压使用颈动脉线, 插管的小弟弟小腿, 以测量 icp, 并刺激 CN 诱导增加的 icp。虽然有几个研究人员对模型进行了改进, 但其重现性仍然是一个问题, 不同的实验室也报告了可变的结果。有几个陷阱仍然存在。
上一篇文章5,6,7,8,9,10描述使用全小弟弟暴露与脱的生殖器海绵体插管。这不是一个最佳的方法, 因为操纵和破坏性解剖导致结构损伤, 这是必不可少的勃起功能。CN 的解剖已被很好地描述为10,11, 但是由于多种因素可能会影响实验结果, 神经的刺激不是最佳的。CN 刺激技术包括通过拉住神经周围的双极钩电极来解除周围组织的神经, 并在每次刺激前干燥神经。这可能导致各种程度的神经损伤和电流泄漏, 从而导致通过刺激替代途径例如、盆底肌肉、膀胱和胃肠道而减少反应或假增加 ICP道12。所有这些因素都限制了重现性。
在我们的研究中, 我们观察到, 麻醉的深度和类型都对 ICP 产生了深远的影响。使用的麻醉剂是戊巴比妥钠, 氯胺酮/甲苯噻嗪或氯胺酮/咪达唑仑注射液, 或异氟醚/氧吸入。
在这里, 我们描述了一个简化的手术方法, 并提供数据支持标准化的实验协议。

<table><tbody><tr><td>Adson forceps</td><td>Fine Science Tool</td><td>11006-12</td><td></td></tr><tr><td>Dumont #7 forceps</td><td>Fine Science Tool</td><td>11271-30</td><td></td></tr><tr><td>Dumont #5 forceps</td><td>Fine Science Tool</td><td>11273-20</td><td></td></tr><tr><td>ToughCut Mayo scissor</td><td>Fine Science Tool</td><td>14110-15</td><td></td></tr><tr><td>Miniature Vannas Spring scissor</td><td>Fine Science Tool</td><td>15006-09</td><td></td></tr><tr><td>Ultra Fine Hemostat</td><td>Fine Science Tool</td><td>13020-12</td><td></td></tr><tr><td>Crile Hemostat</td><td>Fine Science Tool</td><td>13004-14</td><td></td></tr><tr><td>Kwik-Sil Adhesive</td><td>World Precision Instruments</td><td>KWIK-SIL</td><td></td></tr><tr><td>Teflon coated silver wire 0.125 mm</td><td>World Precision Instruments</td><td>AGT0510</td><td></td></tr><tr><td>Elastic wire retractors</td><td>Custom made</td><td></td><td></td></tr><tr><td>Scalpel blade</td><td>Fine Science Tool</td><td>10023-00</td><td></td></tr><tr><td>PE-50 tubing</td><td>Warner Instruments</td><td>64-0753</td><td></td></tr><tr><td>23 G Needle</td><td>Kruuse</td><td>121272</td><td></td></tr><tr><td>SD-9 Square Pulse Stimulator</td><td>Somatco</td><td>1077/183</td><td></td></tr><tr><td>Blood pressure transducer and cable</td><td>World Precision Instruments</td><td>BLPR2</td><td></td></tr><tr><td>Raucotupf Cotton-tipped Applicators</td><td>Lohmann-Raucher</td><td>11966</td><td></td></tr><tr><td>Pro-ophta Ocular Sticks</td><td>Lohmann-Raucher</td><td>16515</td><td></td></tr><tr><td>NaCl 0,9 % 100 mL</td><td>Local pharmacy</td><td></td><td></td></tr><tr><td>Heparin</td><td>Local pharmacy</td><td></td><td></td></tr><tr><td>25 mL Syringe</td><td>Odense University Hospital</td><td></td><td></td></tr><tr><td>Vet eye ointment - Viscotears</td><td>Local pharmacy</td><td></td><td></td></tr><tr><td>silver wires&amp;nbsp;</td><td>Science Products GmbH, Heidelberg, Germany</td><td></td><td></td></tr><tr><td>Silicon Glue&amp;nbsp;</td><td>Kwik-Sil, World Precision Instruments, Sarasota, FL, USA</td><td></td><td></td></tr></tbody></table>

cavernous nerve stimulation and recording of intracavernous pressure in a rat

海绵状神经 (CN) 的刺激和海绵压力的测量已被广泛用于测试和评估勃起功能障碍的治疗方法。然而, 所使用的方法在实验室之间有所不同, 而且仍然存在陷阱。这项研究的目的是描述一种外科技术, 将提供一个可靠的和可重复的模式。通过暴露 ischiocavernosus 肌肉在其插入点上的坐骨股骨, 小弟弟小腿可能是空心的最小解剖和伤害的结构涉及勃起功能。在不需要提升和干燥的情况下, 对 CN 进行反复刺激, 通过使用125µm 双极银电极和生物相容的硅胶来分离电极-神经复合体。该方法通过减少伸展和干燥神经, 提供完全的神经隔离, 消除电漏, 防止刺激的替代途径, 防止 neuropraxia。

使用本协议的建议刺激设置, 在吸入麻醉下, 异氟醚2.0% 氧0.8 升/分, 应产生的结果, 如图 11和图12, 其中有多个回到背面刺激之间的75-80 毫米汞.图 13显示与20分钟的激发相同的稳定响应, 其响应稳定在73-77 毫米汞. 通过冲洗管道和敲击小腿 (图 9) 来测试 ICP 测量的线路。回复基线的快?...

Watch this Scientific Journal Video about Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat at JoVE.com

Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat

本研究介绍了用硅胶胶和海绵压力测量分离神经电极复合物进行海绵状神经刺激的简化手术方法和技术。

大鼠海绵状神经刺激与海绵压力记录

The stimulation of the cavernous nerve (CN) and measurement of intracavernous pressure (ICP) have been used extensively to test and evaluate therapies for ...

cavernous-nerve-stimulation-recording-intracavernous-pressure

Universidad de Cartagena UDEC

Research

JoVE Journal

Medicine

12.4K 次观看.  University of Southern Denmark. 本研究介绍了用硅胶胶和海绵压力测量分离神经电极复合物进行海绵状神经刺激的简化手术方法和技术。

视频: Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat

Epidural Intracranial Pressure Measurement in Rats Using a Fiber-optic Pressure Transducer

Elevated intracranial pressure (ICP) is a significant problem in several forms of ischemic brain injury including stroke, traumatic brain injury and cardiac arrest. This elevation may result in further neurological injury, in the form of transtentorial herniation1,2,3,4, midbrain compression, neurological deficit or increased cerebral infarct2,4. Current therapies are often inadequate to control elevated ICP in the clinical setting5,6,7 . Thus there is a need for accurate methods of ICP measurement in animal models to further our understanding of the basic mechanisms and to develop new treatments for elevated ICP.
In both the clinical and experimental setting ICP cannot be estimated without direct measurement. Several methods of ICP catheter insertion currently exist. Of these the intraventricular catheter has become the clinical 'gold standard' of ICP measurement in humans8. This method involves the partial removal of skull and the instrumentation of the catheter through brain tissue. Consequently, intraventricular catheters have an infection rate of 6-11%9. For this reason, subdural and epidural cannulations have become the preferred methods in animal models of ischemic injury. 
Various ICP measurement techniques have been adapted for animal models, and of these, fluid-filled telemetry catheters10 and solid state catheters are the most frequently used11,12,13,14,15. The fluid-filled systems are prone to developing air bubbles in the line, resulting in false ICP readings. Solid state probes avoid this problem (Figure 1). An additional problem is fitting catheters under the skull or into the ventricles without causing any brain injury that might alter the experimental outcomes. Therefore, we have developed a method that places an ICP catheter contiguous with the epidural space, but avoids the need to insert it between skull and brain. 
An optic fibre pressure catheter (420LP, SAMBA Sensors, Sweden) was used to measure ICP at the epidural location because the location of the pressure sensor (at the very tip of the catheter) was found to produce a high fidelity ICP signal in this model. There are other manufacturers of similar optic fibre technologies13 that may be used with our methodology. Alternative solid state catheters, which have the pressure sensor located at the side of the catheter tip, would not be appropriate for this model as the signal would be dampened by the presence of the monitoring screw. 
Here, we present a relatively simple and accurate method to measure ICP. This method can be used across a wide range of ICP related animal models.

A novel technique to record the pressures within the skull is described. The minimally invasive method uses a fibre-optic pressure sensing system to accurately measure intracranial pressure (ICP) in anaesthetized rats without causing significant brain trauma. The technique may be used in a wide range of experimental models.

硬膜外颅内压测量中使用的光纤压力传感器的大鼠

Elevated intracranial pressure (ICP) is a significant problem in several forms of ischemic brain injury including stroke, traumatic brain injury and ...

科研

医学

A Wireless, Bidirectional Interface for In Vivo Recording and Stimulation of Neural Activity in Freely Behaving Rats

In vivo electrophysiology is a powerful technique to investigate the relationship between brain activity and behavior at a millisecond and micrometer scale. However, current methods mostly rely on tethered cable recordings or only use unidirectional systems, allowing either recording or stimulation of neural activity, but not at the same time or same target. Here, a new wireless, bidirectional device for simultaneous multichannel recording and stimulation of neural activity in freely behaving rats is described. The system operates through a single portable head stage that both transmits recorded activity and can be targeted in real-time for brain stimulation using a telemetry-based multichannel software. The head stage is equipped with a preamplifier and a rechargeable battery, allowing stable long-term recordings or stimulation for up to 1 h. Importantly, the head stage is compact, weighs 12 g (including battery) and thus has minimal impact on the animal&#180;s behavioral repertoire, making the method applicable to a broad set of behavioral tasks. Moreover, the method has the major advantage that the effect of brain stimulation on neural activity and behavior can be measured simultaneously, providing a tool to assess the causal relationships between specific brain activation patterns and behavior. This feature makes the method particularly valuable for the field of deep brain stimulation, allowing precise assessment, monitoring, and adjustment of stimulation parameters during long-term behavioral experiments. The applicability of the system has been validated using the inferior colliculus as a model structure.

A Wireless, Bidirectional Interface for In Vivo Recording and Stimulation of Neural Activity in Freely Behaving Rats

A wireless, bidirectional system for multi-channel neural recordings and stimulation in freely behaving rats is introduced. The system is light and compact, thus having minimal impact on the animal&#180;s behavioral repertoire. Moreover, this bidirectional system provides a sophisticated tool to assess causal relationships between brain activation patterns and behavior.

用于体内的无线双向接口记录和刺激自由行为大鼠的神经活动

In vivo electrophysiology is a powerful technique to investigate the relationship between brain activity and behavior at a millisecond and micrometer ...

行为学

Simultaneous Electrocardiography Recording and Invasive Blood Pressure Measurement in Rats

For studies related to cardiovascular physiology or pathophysiology, blood pressure (BP) and electrocardiography are basic observational parameters. Research focusing on cardiovascular disease models, potential cardiovascular therapeutic targets or pharmaceutical agents requires assessment of systemic arterial pressure and heart rhythm changes. In situations where radio telemetry systems are not available or affordable, the technique of femoral artery cannulation is an alternative way to obtain intra-arterial pressure waveform recordings and systemic BP measurements. This technique is economical and can be performed with standard equipment in animal facilities. However, invasive arterial pressure recording requires cannulation of small arteries, which can be a challenging surgical skill. Here, we present step-by-step protocols for femoral artery cannulation procedures. Key procedures include the calibration of the data acquisition system, tissue dissection and femoral artery cannulation, and setup of the arterial cannulation system for pressure recording. Surface electrocardiography recording procedures are also included. We also present examples of BP recordings from normotensive and hypertensive rats. This protocol allows reliable direct recordings of systemic BP with simultaneous electrocardiography.

Here, we describe a setup for simultaneous recording of electrocardiography and intra-arterial blood pressure (BP) in experimental rats, which can be done with standard equipment in animal facilities and can be applied to physiological or pharmacological studies to investigate pathogenic or therapeutic mechanisms in cardiovascular medicine.

大鼠同时心电图记录及侵入性血压测量

For studies related to cardiovascular physiology or pathophysiology, blood pressure (BP) and electrocardiography are basic observational parameters. ...

Preparation of Peripheral Nerve Stimulation Electrodes for Chronic Implantation in Rats

Peripheral nerve cuff electrodes have long been used in the neurosciences and related fields for stimulation of, for example, vagus or sciatic nerves. Several recent studies have demonstrated the effectiveness of chronic VNS in enhancing central nervous system plasticity to improve motor rehabilitation, extinction learning, and sensory discrimination. Construction of chronically implantable devices for use in such studies is challenging due to rats&#8217; small size, and typical protocols require extensive training of personnel and time-consuming microfabrication methods. Alternatively, commercially available implantable cuff electrodes can be purchased at a significantly higher cost. In this protocol, we present a simple, low-cost method for construction of small, chronically implantable peripheral nerve cuff electrodes for use in rats. We validate the short and long-term reliability of our cuff electrodes by demonstrating that VNS in ketamine/xylazine anesthetized rats produces decreases in breathing rate consistent with activation of the Hering-Breuer reflex, both at the time of implantation and up to 10 weeks after device implantation. We further demonstrate the suitability of the cuff electrodes for use in chronic stimulation studies by pairing VNS with skilled lever press performance to induce motor cortical map plasticity.

Existing approaches for constructing chronically implantable peripheral nerve cuff electrodes for use in small rodents often require specialized equipment and/or highly trained personnel. In this protocol we demonstrate a simple, low-cost approach for fabricating chronically implantable cuff electrodes, and demonstrate their effectiveness for vagus nerve stimulation (VNS) in rats.

大鼠慢性植入的外周神经刺激电极的制备

Peripheral nerve cuff electrodes have long been used in the neurosciences and related fields for stimulation of, for example, vagus or sciatic nerves. ...

神经科学

In Vivo Intracellular Recording of Type-Identified Rat Spinal Motoneurons During Trans-Spinal Direct Current Stimulation

Intracellular recording of spinal motoneurons in vivo provides a &#8220;gold standard&#8221; for determining the cells&#8217; electrophysiological characteristics in the intact spinal network and holds significant advantages relative to classical in vitro or extracellular recording techniques. An advantage of in vivo intracellular recordings is that this method can be performed on adult animals with a fully mature nervous system, and therefore many observed physiological mechanisms can be translated to practical applications. In this methodological paper, we describe this procedure combined with externally applied constant current stimulation, which mimics polarization processes occurring within spinal neuronal networks. Trans-spinal direct current stimulation (tsDCS) is an innovative method increasingly used as a neuromodulatory intervention in rehabilitation after various neurological injuries as well as in sports. The influence of tsDCS on the nervous system remains poorly understood and the physiological mechanisms behind its actions are largely unknown. The application of the tsDCS simultaneously with intracellular recordings enables us to directly observe changes of motoneuron membrane properties and characteristics of rhythmic firing in response to the polarization of the spinal neuronal network, which is crucial for the understanding of tsDCS actions. Moreover, when the presented protocol includes the identification of the motoneuron with respect to an innervated muscle and its function (flexor versus extensor) as well as the physiological type (fast versus slow) it provides an opportunity to selectively investigate the influence of tsDCS on identified components of spinal circuitry, which seem to be differently affected by polarization. The presented procedure focuses on surgical preparation for intracellular recordings and stimulation with an emphasis on the steps which are necessary to achieve preparation stability and reproducibility of results. The details of the methodology of the anodal or cathodal tsDCS application are discussed while paying attention to practical and safety issues.

This protocol describes in vivo intracellular recording of rat lumbar motoneurons with simultaneous trans-spinal direct current stimulation. The method enables us to measure membrane properties and to record rhythmic firing of motoneurons before, during and after anodal or cathodal polarization of the spinal cord.

在跨脊柱直流刺激期间类型识别大鼠脊柱腺瘤的 Vivo 细胞内记录

Intracellular recording of spinal motoneurons in vivo provides a &#8220;gold standard&#8221; for determining the cells&#8217; electrophysiological ...

腹部 VNS 治疗大鼠模型中的炎症

Implantation Surgery for Abdominal Vagus Nerve Stimulation and Recording Studies in Awake Rats

Abdominal vagus nerve stimulation (VNS) can be applied to the subdiaphragmatic branch of the vagus nerve of rats. Due to its anatomical location, it does not have any respiratory and cardiac off-target effects commonly associated with cervical VNS. The lack of respiratory and cardiac off-target effects means that the intensity of stimulation does not need to be lowered to reduce side effects commonly experienced during cervical VNS. Few recent studies demonstrate the anti-inflammatory effects of abdominal VNS in rat models of inflammatory bowel disease, rheumatoid arthritis, and glycemia reduction in a rat model of type 2 diabetes. Rat is a great model to explore the potential of this technology because of the well-established anatomy of the vagus nerve, the large size of the nerve that allows easy handling, and the availability of many disease models. Here, we describe the methods for cleaning and sterilizing the abdominal VNS electrode array and surgical protocol in rats. We also describe the technology required for confirmation of suprathreshold stimulation by recording evoked compound action potentials. Abdominal VNS has the potential to offer selective, effective treatment for a variety of conditions, including inflammatory diseases, and the application is expected to expand similarly to cervical VNS.

作者聚焦:探索腹部 VNS 治疗炎症性疾病

The present protocol describes the surgical technique for implanting an electrode array onto the abdominal vagus nerve in rats, along with methods for chronic electrophysiology testing and stimulation using the implanted device.

腹部迷走神经刺激植入手术和清醒大鼠的记录研究

Abdominal vagus nerve stimulation (VNS) can be applied to the subdiaphragmatic branch of the vagus nerve of rats. Due to its anatomical location, it does ...

Intracavernosal Pressure Recording to Evaluate Erectile Function in Rodents

Erectile dysfunction (ED) is defined as the inability to attain or keep an erection of the penis, and this has become a prevalent male sexual disorder. Rodents are employed by many studies to research the physiology/pathology of erectile function. Erectile function in rodents can be evaluated by measuring the intracavernosal pressure (ICP). In practice, ICP can be monitored following electrical stimulation of the cavernous nerves (CNs). The arterial pressure of the carotid artery (the mean arterial pressure) is used as the reference for ICP. Using ICP recording protocols, many key parameters of erectile function can be measured from the ICP response curve. The ICP measurement provides more information than the apomorphine-induced penile erection test, and is cheaper than telemetric monitoring of the corpus spongiosum penis, making this method the most popular one to evaluate erectile function. However, compared to the easily-performed APO-induced erectile function test, successful ICP recordings require attention to detail, practice, and adherence to the operation method. In this work, an introduction to ICP recording in rats is provided to complement the procedure efficiently.

Intracavernosal pressure recording (ICP) is an important method to evaluate the erectile function of experimental animals. Here, a detailed protocol is demonstrated for the recording procedure of ICP by catheterizing the crura penis and then electrically stimulating the cavernous nerves in rats.

Intracavernosal 压力记录对啮齿动物勃起功能的评价

Erectile dysfunction (ED) is defined as the inability to attain or keep an erection of the penis, and this has become a prevalent male sexual disorder. ...

生物学

Intracavernous Pressure Recording in a Cavernous Nerve Injury Rat Model

The bilateral cavernous nerve (CN) injury rat model has been extensively used to simulate clinical cavernous nerve injury associated with erectile dysfunction (ED) for evaluating the effect of clinical therapeutic methods. However, the methods of CN injury model construction are flawed and varied in the ED research field. It is CN crush injury that is the most commonly used method in recent years. This study aims to provide a detailed description of the procedure of bilateral CN injury rat model construction and measurement of intracavernous pressure (ICP) recording, providing a reliable and reproducible CN injury rat model. This work successfully developed the CN injury method of hemostat crush injury using a syringe needle as hard support and a hemostat with a rubber sleeve. Also, this method concludes that a voltage of 1.0 V, frequency of 20 Hz, and pulse-width of 5 ms are the optimized stimulation parameters for ICP recording in a bilateral CN injury rat model.

This protocol describes developing a stable bilateral cavernous nerve injury rat model of radical prostatectomy associated with erectile dysfunction and intracavernous pressure measurement.

大鼠海绵状神经刺激与海绵压力记录

摘要

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此视频中的章节

硬膜外颅内压测量中使用的光纤压力传感器的大鼠

用于体内的无线双向接口记录和刺激自由行为大鼠的神经活动

大鼠同时心电图记录及侵入性血压测量

大鼠慢性植入的外周神经刺激电极的制备

在跨脊柱直流刺激期间类型识别大鼠脊柱腺瘤的 Vivo 细胞内记录

腹部迷走神经刺激植入手术和清醒大鼠的记录研究

腹部迷走神经刺激植入手术和清醒大鼠的记录研究

腹部迷走神经刺激植入手术和清醒大鼠的记录研究

Intracavernosal 压力记录对啮齿动物勃起功能的评价

Intracavernous Pressure Recording in a Cavernous Nerve Injury Rat Model