We would like to thank the Children&#8217;s Hospital at Montefiore, Department of Pediatrics, for providing support for these studies. We would like to thank the National Alopecia Areata Foundation for providing financial support for conducting studies with C3H/HeJ engrafted mice.

伦理学声明:所有涉及动物的研究必须由该机构IACUC批准（为此如下数据，协议批准了蒙蒂菲奥里IACUC，协议＃11-6-240和＃13-7-100）。动物提供了浅麻醉指示摄影过程中保持他们仍然是唯一的目的，有需要这种协议没有痛苦的过程。 1.采集照片的<ol><li>看设定对焦和现场使用的纸张与印刷文字凝胶成像仪。验证跨区域拍照光源均匀性。为了帮助确保均匀性，使用凝胶成像仪具有一个内置的光源，用于反射摄影。不要使用透照光源，因为这将创建一个不适合进一步的灰度分析动物的剪影。 注:这将将鼠标稍微失焦，其中跨过感兴趣区域（ROI）的区域提供了光学平均，并会有助于减少量子错误的利息非常小（ 即 &lt;10像素）的区域。感兴趣较大区域将不会影响此次要聚焦调整，也不会受到影响，在动物的大小差异。 </li><li>麻醉用氯胺酮（100毫克/毫升）/赛拉嗪（20毫克/毫升）的动物（2:1），因为这提供了快速麻醉效果和快速恢复和最适合拍摄多个动物。 注:麻醉证实当动物仍足以允许摄影。由于动物通常在10-15分钟这个浅麻醉恢复，兽医已经不推荐使用眼药膏的。 </li><li>放置麻醉动物在垂直取向凝胶成像仪（如接近平行越好）。 <ol><li>对于背照片，将动物的四肢伸展俯卧位。 </li><li>对于腹面照片，将动物在仰卧位，照顾这些动物不是横向旋转。 </li&gt; </ol></li><li>将在拍下区域灰度标准。 </li><li>关闭检修门。重要提示:环境光可以引入变化曝光。 </li><li>设置光圈的曝光哪个地方采集的线性范围内的感兴趣的区域（读出的F-停止）。 注意:大多数的系统将显示在图像饱和。 </li><li>拍摄照片。 </li><li>变化的F-停止向其中通过增加或由1减小光圈，这样，无论是标准的和感兴趣的区域保持在采集（参考的线性范围放置的线性范围内的感兴趣的区域的另一曝光设置F-停止）。 </li><li>拍摄照片。 </li><li>一旦拍摄完成后，将动物在加温台和监控，直到他们能保持胸骨斜卧。回到动物的笼子。返回组动物一次所有的动物完全恢复的动物饲养。 </li></ol> 2.定量光吸收的&lt;/ P&gt; <ol><li>使用提供的对动物的图像感兴趣区域标记软件用于凝胶成像仪。 <ol><li>对于整个动物背视图中，使用矩形或椭圆形的图像从上肢到下肢延伸，横向延伸尽可能使得盒的任何部分延伸超过该动物的背部， 如图1A所示 。 注意:一个也标记感兴趣的使用徒手画图工具的区域。 </li><li>对于整个动物腹面观中，使用2矩形:1覆盖骨盆区域，一个覆盖胸部区域， 如图1B所示 。 </li><li>对于感兴趣的， 即，给药位置，标记为适当的较小的区域。 </li></ol></li><li>对灰度吸收基准利率标记区域（S）。 </li><li>从每个感兴趣的标记区域的记录吸收。 </li></ol> 3.分析吸收水平obtained表示感兴趣的区域，可能需要进行归一化的背景标准照片比较。暴露于吸收的关系是双对数（ 即，该关系是log（曝光之间线性）和log（吸收））。使用这种关系，感兴趣的区域的吸收可以是归一化到一个标准的背景值，这将允许吸收直接不同的照片，其中包括那些在不同时间点（一个研究协议范围内，即，连续测量）拍摄之间进行比较。用于执行这些校正的过程详述在任选的步骤3.1和3.2。 <ol><li>日志（曝光）使用来自灰度吸收标准得到的数值（可选）绘制的曲线与日志（吸收）。 </li><li> （可选）调整的变化每张照片的标准如下: <ol><li>选择F-停止阅读和F-STOP（如步骤1.6确定），以供参考（如在下定决心TEP 1.8）。 </li><li>计算标准平均吸收所有的照片在阅读F-停止。这平均值为参考标准值（RSV）。 </li><li>计算在每个照片中的阅读和参考光圈设置之间的吸收差值为标准（Δ-S）和为每个定义的ROI（Δ-ROI-1，Δ-ROI-2 ... ..delta-ROI-X）的</li><li>计算校正吸收每个ROI如下:在阅读F-停止更正吸收率（ROI-X）=吸收率（ROI-X）+（RSV - 吸收的标准，在阅读F-停止）*（Δ-ROI-X / Δ-S） </li></ol></li><li>编译的实验数据，并用标准的统计方法对连续变量进行数据分析（ 即，方差分析，T检验）。 </li></ol>

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Authors have nothing to disclose.

在这份报告中的详细描述，提供了一种新技术，用于量化脱发在啮齿类动物。此技术利用的凝胶成像仪进行图像采集和分析，设备这是在大多数实验室容易获得。测量已被证明是健壮到微小变化在技术（ 图4 - 5），并且是公相关，在治疗模型视觉毛发损失（ 图6）的程度。这些技术已被成功地用于在实验性疗法的评价为脱发17。 ?...

脱发（脱发）可以是心理和情感上令人痛心的事件有多个原因。男性型脱发是脱发的最常见的原因，35岁1影响大约三分之二男性。脱发的一个类似的模式可以在女性多囊卵巢综合征被观察到。在这两种病症中，脱发是雄激素介导的。脱发也可发生自身免疫病，斑秃，从而影响人口2的1.7％。脱发可发生的一些医学治疗，如化疗3的副作用。化疗患者的高比例（65-85％）经历某种程度的脱发4,5。脱发的心理影响已经得到很好的研究，在化疗的设置。化疗引起的脱发会导致焦虑，抑郁，消极的身体形象，降低自尊和幸福6,7减少的感觉。高PE女性癌症患者rcentage（47-58％）认为脱发是化疗最痛苦的方面，以及高达8％的跌幅治疗恐惧脱发4,6。也有在雄激素性脱发的证据来支持治疗，以减少脱发8,9-心理甚至医学后果。同样，斑秃已报道有严重的心理后果2和脱发的斑片状性质可以导致更不愉快的美容效果比脱发的最其他原因。 虽然药物轻度抗雄激素效应（ 即，安体舒通）曾使用了有限的成功为治疗脱发，首先有效的药物治疗脱发的米诺地尔是10。这种降压具有引起毛发生长的观察副作用，现在用作局部治疗许多形式的脱发。然而，反应往往是不完整的，有些科目只显示缓慢荷兰国际集团的脱发，而不是实际的再生10。非那雄胺是一种竞争性拮抗剂II型5α还原酶其阻断睾酮转化为二氢睾酮，致使在局部全身雄激素阻断牺牲改善雄激素性脱发。应答率与长期（10年以上）的治疗是在50％左右11。总体而言，尽管相当多的研究在这方面，目前还没有适当的治疗脱发。 几十年来，科学家和临床医生已经检查测量的头皮头发生长的临床试验的方法。用的药物治疗脱发的发展，出现了用于测量头发的生长，并且具体地，对治疗的反应的可靠的，经济的和微创手段更加需要。图像分析技术对头发密度的患者的脱发症的准确定量，得到在过去的一致和有效的结果使用各种techniqu的ES，包括分析数字化图像12，每根毛发的图像分析和皮损13，和显微镜扫描量化毛传中定义的头皮区域14。 不幸的是，虽然上述的方法提供了效力的改进的评价对头发生长促进干预在临床试验中，这些方法还没有被应用到啮齿动物的研究中的临床前调查。我们的目标是开发一个一致的和可靠的方法来量化脱发小鼠，这将允许研究者能够更准确地评估和比较新的治疗方法对各种形式的脱发。我们已经开发使用的设备在大多数实验室，这将使头发密度的小鼠棕色或黑色的头发快速，可靠的量化一应俱全的方法。这种方法已在化疗诱导的脱发，斑秃，并从waxin脱发的小鼠模型被测试克。详细的协议提出了进行这些测量，包括C57BL / 6的验证数据和C3H /小鼠品系HeJ小鼠。作为这种技术依赖于从在毛干色素检测光的吸收，它不能被用于检测的毛发生长小白鼠或白化小鼠。

<table><tbody><tr><td>KODAK Gel Logic 100 Imaging System</td><td>Eastman Kodak Company, Rochester, NY, USA</td><td></td><td>Gel imager for obtaining and analyzing photographs, must have built in light source for reflective photography.</td></tr><tr><td>The Kodak/Tiffen Q-13 Gray Scale</td><td><a href="http://www.imatest.com/docs/q13/" target="_blank"><img src="/img/new_window.png" /> Imatest</a></td><td></td><td>Greyscale standard</td></tr><tr><td>C57BL/6J Mice</td><td>Jackson Laboratories, Bar Harbor, Maine</td><td></td><td>Mice for representative study</td></tr><tr><td>C3H/HeJ engrafted mouse</td><td>Jackson Laboratories, Bar Harbor, Maine</td><td></td><td>Mice for representative study</td></tr></tbody></table>

a new technique for quantitative analysis of hair loss in mice using grayscale analysis

脱发是它可以发生在许多不同的条件，包括男性型脱发，多囊性卵巢综合征和斑秃脱发的常见形式。脱发，也可能发生由于化疗的癌症患者的副作用。在这项研究中，我们的目标是开发一个一致的和可靠的方法来量化脱发小鼠，这将允许研究者准确地评估和比较新的治疗方法，这些不同形式的脱发。该方法利用一种标准的凝胶成像仪获取和处理的小鼠的图像，测量光吸收，这发生在粗糙成比例的黑色（或灰色）的头发上的鼠标的量。已量化以这种方式的数据然后可以使用标准的统计技术（ 即，方差分析，T检验）分析。这种方法是在化疗诱导的脱发，斑秃和脱发的小鼠模型从打蜡测试。在这份报告中，详细的协议是PRESented用于执行这些测量，其中包括来自C57BL / 6的验证数据和C3H /小鼠HeJ小鼠品系。这种新技术提供了许多优点，包括应用的相对简单性，依赖于设备这是在大多数研究实验室容易获得的，并且施加一个客观，定量评估这比主观评价更加健壮。改善头发生长的量化小鼠会改善脱发模型研究和促进有前途的新治疗方法的评价在临床前研究。

用的C3H / HeJ小鼠植入小鼠，脱发的小鼠模型斑秃16这种技术进行了验证。这些动物发展出进步逐渐随着时间的推移全球性脱发。然而，脱发发生在补丁，并且可以变化从一个鼠标到下，引入相当大的变化，并且会阻碍的定性评估。该模型提供了一个机会，在对小鼠的有效性进行评估的不同位置，以测试光密度的测量值之间的相关性。 如果使用的是第一个问题是措施不?...

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A New Technique for Quantitative Analysis of Hair Loss in Mice Using Grayscale Analysis

Alopecia is a common form of hair loss which can occur in many different conditions, including as a side-effect of chemotherapy. We have developed a method to quantify hair loss in mice, utilizing a standard gel imager to perform a grayscale analysis, to facilitate study of promising new alopecia therapies.

新技术脱发的定量分析小鼠使用灰度分析

Alopecia is a common form of hair loss which can occur in many different conditions, including male-pattern hair loss, polycystic ovarian syndrome, and ...

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8.9K 次观看.  Children's Hospital at Montefiore. Alopecia is a common form of hair loss which can occur in many different conditions, including as a side-effect of chemotherapy. We have developed a method to quantify hair loss in mice, utilizing a standard gel imager to perform a grayscale analysis, to facilitate study of promising new alopecia therapies.

视频: A New Technique for Quantitative Analysis of Hair Loss in Mice Using Grayscale Analysis

Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology

Skin is a highly heterogeneous tissue. Intra-dermal structures include hair follicles, arrector pili muscles, epidermal specializations (such as Merkel cell clusters), sebaceous glands, nerves and nerve endings, and capillaries. The spatial arrangement of these structures is tightly controlled on a microscopic scale - as seen, for example, in the orderly arrangement of cell types within a single hair follicle - and on a macroscopic scale - as seen by the nearly identical orientations of thousands of hair follicles within a local region of skin. Visualizing these structures without physically sectioning the skin is possible because of the 2-dimensional geometry of this organ. In this protocol, we show that mouse skin can be dissected, fixed, permeabilized, stained, and clarified as an intact two dimensional object, a flat mount. The protocol allows for easy visualization of skin structures in their entirety through the full thickness of large areas of skin by optical sectioning and reconstruction. Images of these structures can also be integrated with information about position and orientation relative to the body axes.

Mammalian skin contains a diverse array of structures - such as hair follicles and nerve endings - that exhibit distinctive patterns of spatial organization. Analyzing skin as a flat mount takes advantage of the 2-dimensional geometry of this tissue to produce full-thickness high-resolution images of skin structures.

小鼠皮肤及其毛囊图案和感觉轴突形态分析中的应用平山成像

Skin is a highly heterogeneous tissue. Intra-dermal structures include hair follicles, arrector pili muscles, epidermal specializations (such as Merkel ...

科研

神经科学

Combined Recording of Mechanically Stimulated Afferent Output and Nerve Terminal Labelling in Mouse Hair Follicle Lanceolate Endings

A novel dissection and recording technique is described for monitoring afferent firing evoked by mechanical displacement of hairs in the mouse pinna. The technique is very cost-effective and easily undertaken with materials commonly found in most electrophysiology laboratories, or easily purchased. The dissection is simple and fast, with the mechanical displacement provided by a generic electroceramic wafer controlled by proprietary software. The same software also records and analyses the electroneurogram output. The recording of the evoked nerve activity is through a commercial differential amplifier connected to fire-polished standard glass microelectrodes. Helpful tips are given for improving the quality of the preparation, the stimulation and the recording conditions to optimize recording quality. The system is suitable for assaying the electrophysiological and optical properties of lanceolate terminals of palisade endings of hair follicles, as well as the outcomes from their pharmacological and/or genetic manipulation. An example of combining electrical recording with mechanical stimulation and labeling with a styryl pyridinium vital dye is given.

A simple and novel technique for recording afferent discharge due to mechanical stimulation of lanceolate terminals of palisade endings innervating mouse ear skin hair follicles is presented.

机械刺激传入输出和神经末梢标签联合录制在小鼠毛囊披针形结局

A novel dissection and recording technique is described for monitoring afferent firing evoked by mechanical displacement of hairs in the mouse pinna. The ...

Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin

A novel dissection and recording technique is described for optical monitoring staining and de-staining of lanceolate terminals surrounding hair follicles in the skin of the mouse pinna. The preparation is simple and relatively fast, reliably yielding extensive regions of multiple labeled units of living nerve terminals to study uptake and release of styryl pyridinium dyes extensively used in studies of vesicle recycling. Subdividing the preparations before labeling allows test vs. control comparisons in the same ear from a single individual. Helpful tips are given for improving the quality of the preparation, the labeling and the imaging parameters. This new system is suitable for assaying pharmacologically and mechanically-induced uptake and release of these vital dyes in lanceolate terminals in both wild-type and genetically modified animals. Examples of modulatory influences on labeling intensity are given.

Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin

Here we describe a novel preparation for imaging live lanceolate sensory terminals of palisade endings that innervate mouse ear skin hair follicles during staining and destaining with styryl pyridinium dyes.

在毛囊披针形结局生活神经末端标记的光学监测<em&gt;离体</em&gt;鼠耳皮肤

A novel dissection and recording technique is described for optical monitoring staining and de-staining of lanceolate terminals surrounding hair follicles ...

Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models

Although bioluminescence imaging (BLI) shows promise for monitoring tumor burden in animal models of cancer, these analyses remain mostly qualitative. Here we describe a method for bioluminescence imaging to obtain a semi-quantitative analysis of tumor burden and treatment response. This method is based on the calculation of a luminoscore, a value that allows comparisons of two animals from the same or different experiments. Current BLI instruments enable the calculation of this luminoscore, which relies mainly on the acquisition conditions (back and front acquisitions) and the drawing of the region of interest (manual markup around the mouse). Using two previously described mouse lymphoma models based on cell engraftment, we show that the luminoscore method can serve as a noninvasive way to verify successful tumor cell inoculation, monitor tumor burden, and evaluate the effects of in situ cancer treatment (CpG-DNA). Finally, we show that this method suits different experimental designs. We suggest that this method be used for early estimates of treatment response in preclinical small-animal studies.

Bioluminescence imaging is a well-known tool for localizing tumors and metastases, but quantification of these images often requires complex calculations and particular instruments. We describe the easy-to-use luminoscore method, based on precise acquisition conditions, requiring no calculations, and enabling tumor burden and treatment response to be monitored in mouse models.

对小鼠淋巴瘤模型监测肿瘤进展和治疗效果生物发光基于肿瘤定量方法

Although bioluminescence imaging (BLI) shows promise for monitoring tumor burden in animal models of cancer, these analyses remain mostly qualitative. ...

医学

Analysis of Craniomaxillofacial Malformations in Mice Using Three-dimensional Microcomputed Tomography

To model craniofacial malformations caused by vitamin A deficiency (VAD), we expressed a dominant-negative retinoid receptor mutation in osteoblasts to specifically inhibit RAR transcriptional activity in mice. This approach allowed us to investigate the effects of VAD on cranial hypomineralization, mandibular deformity, and clavicular hypoplasia in clinical cases. In this study, microcomputed tomography (microCT) scanning of the craniomaxillofacial region of mice represented a valuable tool for studying the growth and development of this animal model. The manual estimation of images is both time-consuming and inaccurate. Hence, here, we present a straightforward, efficient, and accurate approach for segmenting and quantifying the microCT images of each craniomaxillofacial bone. MicroCT software was used to slice the mandible, frontal bone, parietal bone, nasal bone, premaxilla, maxilla, interparietal bone, and occipital bone of mice and measure their corresponding lengths and widths. This segmentation method can be applied to study growth and development in developmental biology, biomedicine, and other related sciences and allows researchers to analyze the effects of genetic mutations on individual craniofacial bones.

Here, we describe in depth a method, which is based on microcomputed tomography, to segment and measure 3D models of craniomaxillofacial bones in mice, for better assessment of craniomaxillofacial bone development in mice than what is possible with current methods.

使用三维显微计算机断层扫描分析小鼠颅颌面畸形

To model craniofacial malformations caused by vitamin A deficiency (VAD), we expressed a dominant-negative retinoid receptor mutation in osteoblasts to ...

发育生物学

Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration

Hair follicle morphogenesis, a complex process requiring interaction between epithelia-derived keratinocytes and the underlying mesenchyme, is an attractive model system to study organ development and tissue-specific signaling. Although hair follicle development is genetically tractable, fast and reproducible analysis of factors essential for this process remains a challenge. Here we describe a procedure to generate targeted overexpression or shRNA-mediated knockdown of factors using lentivirus in a tissue-specific manner. Using a modified version of a hair regeneration model 5, 6, 11, we can achieve robust gain- or loss-of-function analysis in primary mouse keratinocytes or dermal cells to facilitate study of epithelial-mesenchymal signaling pathways that lead to hair follicle morphogenesis. We describe how to isolate fresh primary mouse keratinocytes and dermal cells, which contain dermal papilla cells and their precursors, deliver lentivirus containing either shRNA or cDNA to one of the cell populations, and combine the cells to generate fully formed hair follicles on the backs of nude mice. This approach allows analysis of tissue-specific factors required to generate hair follicles within three weeks and provides a fast and convenient companion to existing genetic models.

Tissue-specific analysis of a hair follicle regeneration model using lentivirus to mediate gain- or loss-of-function.

在毛发再生的上皮 - 间充质信号的快速遗传分析

Hair follicle morphogenesis, a complex process requiring interaction between epithelia-derived keratinocytes and the underlying mesenchyme, is an ...

生物学

Digital Planimetry for Assessing Wound Closure Kinetics in a Mouse Model

Chronic wounds, due to their high prevalence, are a serious global health concern. Effective therapeutic strategies can significantly accelerate healing, thereby reducing the risk of complications and alleviating the economic burden on healthcare systems. Although numerous experimental studies have investigated wound healing, most rely on qualitative observations or quantitative direct measurements. The objective of this study was to standardize an indirect wound measurement method using digital planimetry, incorporating digital scaling and segmentation. This approach addresses the lack of detailed, step-by-step methodologies for accurate wound assessment. A photodocumentation booth was designed and constructed, and computer-assisted digital planimetry tools were employed to minimize variability in measurements of the wound area, perimeter, and the distance from the wound center to its edges. A circular traumatic wound (5 mm in diameter) was created on the dorsal midline at the shoulder blade level of male CD1 mice (n = 4, 10 weeks old, 30-35 g). Wound evolution was photodocumented for 14 days using the custom-designed photo booth, which controlled lighting conditions, focal distance, and subject positioning. Scaling and wound measurements were performed using segmentation in ImageJ software, and statistical analysis was conducted using statistical analysis software. The kinetics of wound closure showed a slight increase in wound size and perimeter between day 0 and day 2, followed by a gradual decrease until complete closure by day 14. The photodocumentation booth and computer-assisted digital planimetry enabled quantitative measurements with minimal variability. In conclusion, these tools provide a reliable and reproducible method for evaluating wound closure kinetics in pre-clinical models.

Wounds represent a global health challenge. This study developed a standardized photo booth utilizing digital planimetry to minimize wound measurement variability. Monitoring wounds in mice over 14 days revealed an initial increase in wound area and perimeter, followed by gradual closure. This methodology may aid in evaluating wound closure kinetics in pre-clinical models.

用于评估小鼠模型中伤口闭合动力学的数字平面测量法

Chronic wounds, due to their high prevalence, are a serious global health concern. Effective therapeutic strategies can significantly accelerate healing, ...

生物工程

Non-invasive Skeletal Muscle Quantification in Small Animals Using Micro-computed Tomography

Skeletal muscle size, mass, and composition are critical properties for studying metabolic and muscle-related diseases, as they directly impact the understanding of disease progression and treatment outcomes. Quantifying a live animal's lean, adipose, and skeletal mass is important in metabolic, physiology, pharmacologic, and geroscience studies. However, obtaining accurate body composition measurements, especially of lean mass, remains challenging due to the inherent limitations of conventional assessment techniques. Micro-computed tomography (micro-CT) is a non-invasive radiological technique that enables high-resolution visualization of internal structures in small animal models. A standardized micro-CT method can significantly enhance translational research with more reliable and impactful results, particularly during aging studies or at different time points within the same animal. Despite its potential, the lack of standardization in image acquisition and analysis methods significantly hinders the comparability of results across different studies. Herein, we present a comprehensive and detailed low-cost protocol for lean mass analysis using micro-CT to address these challenges and promote consistency in research involving small animal models.

This protocol uses micro-computed tomography to enable the cost-effective quantification of lean mass, including skeletal muscle and visceral tissue, adipose tissue, and skeletal tissue in small animals. It distinguishes between lean and adipose tissue, which offers significant advantages for biomedical research, particularly in translational research in small animals.

使用显微计算机断层扫描对小动物进行无创骨骼肌量化

Skeletal muscle size, mass, and composition are critical properties for studying metabolic and muscle-related diseases, as they directly impact the ...

Non-Invasive Visualization of Nailbed Microvascular Morphology in Mice Using Capillaroscopy

Imaging microcapillary networks of the skin in humans using nailfold capillaroscopy (NFC) has underscored the importance of microcirculation as a target organ system in critical systemic illnesses. Nailfold capillaroscopy is applied clinically to detect peripheral microvascular dysfunction and abnormalities in a range of systemic conditions, including rheumatic, cardiac, ocular (e.g., glaucoma), and endocrine disorders (e.g., hypertension and diabetes mellitus). NFC is useful not only in detecting peripheral systemic microvasculature disruption but also in assessing drug efficacy. However, translating clinical NFC findings to animal disease models can be challenging. Detecting microvascular dysfunction or abnormalities in animals is often invasive (e.g., endoscopic), carried out ex vivo (e.g., post-mortem imaging of tissues), or expensive, requiring specialized equipment such as those used in microcomputed tomography and photoacoustic imaging techniques. Developing quick, non-invasive, and inexpensive techniques to image peripheral microvasculature in animal models of disease is warranted to decrease research expenses and increase translatability to the clinic.
Capillaroscopy has previously been used to visualize the nailfold microvasculature in animal models, including in guinea pigs and mice, thus demonstrating the capability of capillaroscopy as a non-invasive imaging tool in animal models. This study provides a protocol that applies capillaroscopy to a mouse nailbed, allowing researchers to easily and inexpensively assess the morphology of its microvasculature. Representative images of typical nailbed microvascular architecture in wild-type mice using two commonly used laboratory strains, SV129/S6 and C57/B6J, are provided. Further studies using this method are essential for applying nailbed capillaroscopy to a wide range of mouse disease models with peripheral microvascular abnormalities.

Capillaroscopy is an accessible tool for direct, inexpensive, and non-invasive visualization of microvasculature. The goal of this protocol is to enable researchers to use capillaroscopy for the visualization of peripheral microvascular morphology in the nailbeds of mice.

使用毛细血管镜检查小鼠甲床微血管形态的无创可视化

Imaging microcapillary networks of the skin in humans using nailfold capillaroscopy (NFC) has underscored the importance of microcirculation as a target ...

A Technique for Human Skin Grafting in a Mouse Model

Source: Moss, M. I. et al., <a href="https://appjove.unicartagenaproxy.elogim.com/v/64040">Xenograft Skin Model to Manipulate Human Immune Responses&#160;In Vivo.</a>&#160;J. Vis. Exp. (2022)
This video demonstrates a method for grafting human skin onto an immunodeficient mouse with a suppressed neutrophil response. The method involves applying human split-thickness skin to the mouse&#39;s graft bed, sealing it, and covering it for recovery. Eventually, the human skin adheres to the mouse&#39;s skin, establishing vascularization and circulation, resulting in a successful skin xenograft.

小鼠模型中的人体皮肤移植技术

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
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