笔者想承认脑与行为研究基金会的慷慨支持。

该协议如下规定由道格拉斯研究所和伦理委员会的指导方针。 1.患者准备<ol><li>当参与者到达时，解释实验，并取得签名已获得伦理委员会批准的机构的伦理审查委员会的知情同意书上。 </li><li>用红铅笔在鼻（鼻根）的桥梁，在眉毛之间做一个记号。使用卷尺，从鼻根测量到头骨上，INION的下基部。由滑动的手的颈部直到参与者的头部的背面的凸块被认为识别该位置。 </li><li>取1/10鼻根和INION之间的测量，并作出标记向上邻近的鼻根发丝，小心，以确保这些测量都是在用鼻线。 </li><li>包裹头卷尺（4色带）头部周围的标记的水平在步骤1.3中进行。确定帽尺寸根据所在的带的非着色端满足着色端:使用一蓝帽子如果它穿过在蓝色区域，使用蓝红帽如果它穿过蓝色和红色区域等之间</li></ol> 2.电极放置<ol><li>将盖子上的参与者的头上。排队的两个电极在用眼帽的前面。插入一次性海绵盘上的正面电极（FP1和FP2），以保持电 - 凝胶扩散。 </li><li>抓住两个正面电极对近发际线的标记和拉盖在头上。确保盖子完全吻合的参与者头上。 </li><li>将耳电极上的参与者的耳垂为参比电极。用酒精清洁的耳垂。用棉签涂药，脑电波及磨料皮肤跃跃欲试凝胶到耳垂，方便性。 </li><li>拿注射器和电解克填充埃尔。然后，挤胶到耳电极。小心地将凝胶填充耳电极上的参与者的耳垂。 </li><li>附上从帽导线和耳电极脑电图放大器。设置放大器，以20000的增益。附加线束与盖带的盖，确保它被紧贴地保持在盖就位。 </li><li>开始应用电解质凝胶到接地电极，其在所述盖的中央前发现。电解质凝胶增加头皮和电极之间的电连接性。 </li><li>使用注射器挤电解质凝胶成电极。开始通过将注射器插入电极和摇动，来回，直到它与头皮接触。然后，同时挤压注射器拉起，创造凝胶的垂直列。 </li><li>对所有的电极重复步骤2.7。 </li><li>研磨用钝针头在电极下方的头皮。做到这一点，而observi纳克的计算机监视器，其中应该显示电极的示意图。瞄准直到屏幕上相应的电极变成黑色的划伤头皮。建议先从耳电极，然后移动到接地电极。 </li></ol> 3.脑电图EEG实验<ol><li>阅读说明在实验过程中的主题。打开电脑上的脑电信号采集软件。 </li><li>观察电极的静止活性。排除为"坏"的电极，例如平线的信号，或过度活跃信号。如果有任何，重复步骤2.7和2.9，以减少阻抗。 strong&gt;注意:阻抗，它使用的是30赫兹电流测量，应保持低于5千欧。 </li><li>开始录制后的电极信号很好看，也没有平线信号或过度活跃的信号。寻找任何myograms或眼睛运动，并且确保该partici七分裤止息闪烁，紧绷的下巴和额头的肌肉，因为这些会产生过大的噪声数据。处于静止状态，也可以是β和α节律本。 </li><li>开始第二个计算机上的实验。实验设计的时序是非常重要的。仔细观察，以确保实验软件，如E-总理，发出"标记"，以表明刺激发病。这是至关重要的，因为刺激发病通常被用作用于在ERP系统范例的事件的参考点。 </li><li>一旦试验完成，确保数据已经saved-根据所使用的软件的这一过程将有所不同。小心地取下帽子，帮助头发洗涤和干燥的参与者。 </li><li>分离来自放大器的帽和耳的电极，除去一次性海绵盘，并清洗下用棒或棉棒的末端自来水盖和耳的电极。用温和的肥皂或洗发水，还有牙刷，轻轻CL耳来自电极的凝胶。彻底冲洗。使设备在空气中干燥。 </li></ol> 4.数据处理<ol><li>以获得从脑电图ERP成分，确定哪个时期进行研究。例如，为了计算N400振幅，使用平均电压在300-500毫秒的时间窗口进行计算。 </li><li>前平均脑电图时代，拒绝过度的眼球运动或放大器饱和度试验。根据不同的实验范例，它可以是有关拒绝对应于不正确的反应试验。 </li><li>平均后，过滤的ERP和绘制数据（见动画图1为利用Matlab，EEGLab和ERPLab数据处理的具体说明）。 </li></ol>

<ol>
	<li>Debruille, J. B., Rodier, M., Prevost, M., Lionnet, C., Molavi, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+a+small+dose+of+olanzapine+on+healthy+subjects+according+to+their+schizotypy:+an+ERP+study+using+a+semantic+categorization+and+an+oddball+task.">Effects of a small dose of olanzapine on healthy subjects according to their schizotypy: an ERP study using a semantic categorization and an oddball task.</a> Europeam Neuropsychopharmacology. 23 (5), 339-350 (2013).</li><li>Bressler, S. L., Arbib, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The Handbook of Brain Theory and Neural Networks. , 412-415 (2002).</li><li>Peterson, N. N., Schroeder, C. E., Arezzo, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neural+generators+of+early+cortical+somatosensory+evoked+potentials+in+the+awake+monkey.">Neural generators of early cortical somatosensory evoked potentials in the awake monkey.</a> Electroencephalography and Clinical Neurophysiology. 96 (3), 248-260 (1995).</li><li>Blackwood, D. H., Muir, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cognitive+brain+potentials+and+their+application.">Cognitive brain potentials and their application.</a> The British Journal of Psychiatry Supplement. 9, 96-100 (1990).</li><li>Debruille, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+N400+potential+could+index+a+semantic+inhibition.">The N400 potential could index a semantic inhibition.</a> Brain Research Reviews. 56 (2), 472-477 (2007).</li><li>Kutas, M., Hillyard, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A.Event-Related+Brain+Potentials+(ERPs)+Elicited+by+Novel+Stimuli+during+Sentence+Processing.">A.Event-Related Brain Potentials (ERPs) Elicited by Novel Stimuli during Sentence Processing.</a> Annals of the New York Academy of Sciences. 425 (1), 236-241 (1984).</li><li>Holcomb, P. J., Kounios, J., Anderson, J. E., West, W. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dual-coding,+context-availability,+and+concreteness+effects+in+sentence+comprehension:+an+electrophysiological+investigation.">Dual-coding, context-availability, and concreteness effects in sentence comprehension: an electrophysiological investigation.</a> Journal of Experimental Psychology: Learning, Memory, and Cognition. 25, 721-742 (1999).</li><li>Renoult, L., Brodeur, M. B., Debruille, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Semantic+processing+of+highly+repeated+concepts+presented+in+single-word+trials:+electrophysiological+and+behavioral+correlates.">Semantic processing of highly repeated concepts presented in single-word trials: electrophysiological and behavioral correlates.</a> Biological Psychology. 84, 206-220 (2010).</li><li>Prevost, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Schizotypal+traits+and+N400+in+healthy+subjects.">Schizotypal traits and N400 in healthy subjects.</a> Psychophysiology. 47, 1047-1056 (2010).</li><li>Sur, S., Sinha, V. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Event-related+potential:+An+overview.">Event-related potential: An overview.</a> Industrial Psychiatry Journal. 18 (1), 70-73 (2009).</li><li>Condray, R., Siegle, G. J., Cohen, J. D., van Kammen, D. P., Steinhauer, S. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Automatic+activation+of+the+semantic+network+in+schizophrenia:+evidence+from+event-related+brain+potentials.">Automatic activation of the semantic network in schizophrenia: evidence from event-related brain potentials.</a> Biological Psychiatry. 54, 1134-1148 (2003).</li><li>Condray, R., Steinhauer, S. R., Cohen, J. D., van Kammen, D. P., Kasparek, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modulation+of+language+processing+in+schizophrenia:+effects+of+context+and+haloperidol+on+the+event-related+potential.">Modulation of language processing in schizophrenia: effects of context and haloperidol on the event-related potential.</a> Biological Psychiatry. 45, 1336-1355 (1999).</li><li>Light, G. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electroencephalography+(EEG)+and+event-related+potentials+(ERPs)+with+human+participants.">Electroencephalography (EEG) and event-related potentials (ERPs) with human participants.</a> Current Protocols in Neuroscience. , 21-24 (2010).</li><li>Luck, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> An Introduction to the Event-Related Potential Technique. , (2005).</li><li>Luck, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> An Introduction to the Event-Related Potential Technique. , (2005).</li></ol>

作者什么都没有透露。

在我们对N400抗精神病药物治疗的效果的调查，我们发现，在安慰剂条件下，前N400s是在具有高schizotypy比低schizotypy受试者个体大。然而，在服药状况，额叶中央N400s较小，但仅在高schizotypy的个人。因此，在服药被视为高分裂型个体，这证实了先前的研究说明与抗精神病药物治疗的精神分裂症患者，相对于服用安慰剂的患者11,12较小的前N400s减少这些大额叶中央N400s。在具有低schizotypy个人，有药物没有影响。这些研究结果与正确执行协议的获得，以及对收集清晰和准确的数据的重要性发言。 为了确保准确，有效的结果，有几个关键点需要注意。对获得良好的信号，这是至关重要的盖是紧密地贴合，并放置使矢状电极是恰好在中间，该阻抗被调整。帽过大可降低录音质量，因为这将是更难减少阻抗13。同样重要的是，与会者明白，他们必须避免过度的运动，眨眼，或弯曲的表面和颚肌，因为这些操作将引入变化，脑电图痕迹和可能使数据的解释很困难14。实验结束后，设备必须正确清洗，以确保该电极不被堵塞用凝胶残余物，这可能会影响以后的信号采集。 如果有信号的问题，如噪音或平线，检查，以确保地面和参比电极连接是否正确。减少这些电极的阻抗可以降低噪声，因此，如果有在所述的连接问题，重新应用凝胶和重新从头电极下方的头皮。如果有myograms，让被摄体，然后再继续放松。在设计该实验中，它是相关于ERP的记录，该实验设计占时序方面，如刺激呈现和短暂的时间周期，允许参与者闪烁的持续时间。 在开始一个脑电图实验，以了解有关的限制是重要的。空间分辨率相对较差可能是要考虑的事情，以及使用脑电图阐明皮质下活动的难度。通过闪烁和肌肉的活动引入的噪声也是不利的，并获得了神经信号，因为他们通过脑脊液，脑膜和颅骨传递被弄脏。这些限制可能会用其他影像学方法independently-，如磁共振成像，近红外光谱＆＃解决8211;或者通过脑电图与这些替代方案的完美组合。然而，如前面提到的那样，这些方法也携带其自身的局限性。必要的对比条件和功能磁共振成像缺乏基线和极性都是明显的例子。相对于另一种脑成像技术，ERP系统记录有许多优势 - 最特别是其显着的时间分辨率，这是在毫秒数量级。这也是非侵入性和最小不舒服的参与者，并没有与任何显著风险或危险有关。 ERP的记录是经济上可行的，特别是相对于诸如功能性磁共振成像（fMRI）和正电子发射断层扫描（PET）15其它神经成像技术。它提供了一个外表成中发生的认知，感觉和运动过程中的神经元的活动，并在将来，可以适用于探索前意识流程。

而神经影像学的进步已经不可否认增强我们的大脑的功能的理解，这些进步有一个基本上静止强调阐明脑的结构。这随后叶脑网络相对笼罩在朦胧的动态时间特性，神经影像学那些解决这些时间方面，如磁共振成像和近红外光谱法（NIRS），产生相比于脑电图的那是不够的时间分辨率的方法。因此，这是相关的，以便更好地理解和使用ERP的使用记录，其能够产生大量的有意义的信息，否则将是未知的。 ERP的记录是一种非侵入性的分析工具，它允许认知神经科学家跟踪神经活动的快速瞬时调制是由认知，感觉和运动过程中引出。它包括将头皮上的电极捕捉到大n的电突触后的反应euronal人群2至特定的事件或刺激。有效保护率是指在时间上被锁定到的感觉，运动或认知活动的脑电图变化，并且被认为是代表信息处理3中产生的突触后电位的总和。这些ERP的波形的特征是它们的等待时间，振幅，极性和头皮分布，提供了神经学数据的丰富阵列可能刺激进一步的深入了解，有助于认知的神经连接。 ERP系统的记录和脑电图的目标（EEG）是获得关于涉及高阶的基本神经过程，复杂的认知操作4的信息。事件相关电位由它们的负或正的波动，以及其在头皮上的位置以及它们的刺激后发病出现的时间限定。各种的ERP已经与各方面的联系认知过程。例如，我们一直在寻找在N400的事件相关脑电位5，即引起400ms的有意义的刺激如词语6，且其在头皮上的分布是已知的依赖于语义类别发病后的负波形这些刺激。在N400是语义激活的索引，并且有研究表明，它具有用于词语较大幅值激活多个表示，如混凝土的话（ 例如 ，香蕉），该激活视觉和口头陈述，相比于抽象的词语（ 例如 ，想法），后者激活只是口头表示7,8。研究也支持，即使是轻微的精神分裂症的症状，如schizotypy尺度衡量，都与N400 9的异常过度的语义激活有关的想法。因此，我们正在探索抗精神病药物，从而降低精神分裂症的症状表现，无论是标准化本身异常在N400的健康个体中具有高水平schizotypy的语义激活。这里，在一个语义分类的范例的使用事件相关电位的是有利的，对于研究在N400的抗精神病药物的效果。 由于ERP记录是一种非侵入性且相对经济的方法来评估神经功能，它可以在广泛的领域得到应用，就证明了大量的认知神经科学，神经学，神经心理学，心理语言学ERP研究和认知心理学。它的多功能性使调查人员询问有关的神经活动的相对时序相关问题的研究在一个大的各种领域，包括语言，认知，以及各种精神疾病10，如精神分裂症，双相情感障碍，抑郁症的研究，以及酒精依赖症和它在神经成像的替代方法，如FUNCT提供了很大的优势有理磁共振成像（fMRI）。其中一些包括它的优良的时间分辨率，其阐述的大脑活动到毫秒。 ERP的记录也可以直接索引兴奋性突触后电位（EPSP的）和抑制性突触后电位（IPSPs），通过许多脑计算执行，一些功能磁共振成像不会做。 ERP的记录的另一个优点是，它提供了区分兴奋活性（神经元去极化）的抑制活性（神经hyperpolarizations）的可能性，而在磁共振成像，更大的信号没有被明确地在这两者之间区分。此外，企业资源规划的方法不需要造影条件:原料的ERP可以检查对于只有一个实验条件下，从fMRI的区别，其中的控制条件，必须从实验条件中减去，导致不确定性相关联的实验或对照条件的观测。 ERP录音的效用，相对成本效益和相关优势，提供在认知神经科学中使用的ERP作为一种方法来跟踪神经活动的快速瞬时变化强有力的理由。其次是一步一步的指导，运行脑电图实验，并记录事件相关电位的基础知识。

<table><tbody><tr><td>EEG acquisition software (Scan 4.5)</td><td>Neuroscan</td><td>http://www.neuroscan.com/scan.cfm</td><td></td></tr><tr><td>Digital EEG Amplifier (NuAmp)</td><td>Neuro Scan Labs</td><td>&lt;a href=&quot;http://www.neuroscan.com/documents/AF573-01%20CURRY%20NuAmpsExpressPRESSD4.pdf&quot;&gt;http://www.neuroscan.com/documents/AF573-01%20CURRY%20NuAmpsExpressPRESSD4.pdf&lt;/a&gt;</td><td></td></tr><tr><td>2 computers</td><td></td><td></td><td></td></tr><tr><td>Matlab</td><td>The MathWorks, Inc</td><td>&lt;a href=&quot;http://www.mathworks.com/products/matlab/&quot;&gt;http://www.mathworks.com/products/matlab/&lt;/a&gt;</td><td></td></tr><tr><td>EEGLab Matlab toolbox</td><td></td><td>http://sccn.ucsd.edu/eeglab/</td><td></td></tr><tr><td>ERPLAB Toolbox</td><td></td><td>&lt;a href=&quot;http://erpinfo.org/erplab&quot;&gt;http://erpinfo.org/erplab&lt;/a&gt;</td><td></td></tr><tr><td>Stimulus generation software</td><td>E-Prime</td><td></td><td></td></tr><tr><td>ECI Electrode cap</td><td>Electro-cap International, Inc</td><td>http://www.electro-cap.com/index.cfm/caps/</td><td></td></tr><tr><td>Special Head Measuring Tape (4 Colour ribbon)</td><td>Electro-cap International, Inc</td><td>http://www.electro-cap.com/index.cfm/supplies/</td><td></td></tr><tr><td>Disposable Sponge Disks</td><td>Electro-cap International, Inc</td><td>http://www.electro-cap.com/index.cfm/supplies/</td><td></td></tr><tr><td>EEG Abrasive Skin Prepping Gel (Nuprep)</td><td>Weaver and Company</td><td>http://www.weaverandcompany.com/nuprep.html</td><td></td></tr><tr><td>Body harness</td><td>Electro-cap International, Inc</td><td>http://www.electro-cap.com/index.cfm/supplies/</td><td></td></tr><tr><td>Cap straps</td><td>Electro-cap International, Inc</td><td>http://www.electro-cap.com/index.cfm/supplies/</td><td></td></tr><tr><td>Electro-gel</td><td>Electro-cap International, Inc</td><td></td><td></td></tr><tr><td>Blunt needle (BD Vacutainer PrecisionGlide Multiple Sample Needle)</td><td>Becton, Dickinson and Company</td><td>367211; see http://www.bd.com/resource.aspx?IDX=7559</td><td></td></tr><tr><td>Syringe</td><td>Electro-cap International, Inc</td><td>http://www.electro-cap.com/index.cfm/supplies/</td><td></td></tr><tr><td>2 Ear Electrodes</td><td>Electro-cap International, Inc</td><td>http://www.electro-cap.com/index.cfm/supplies/</td><td></td></tr><tr><td>Alcohol wipes</td><td></td><td></td><td></td></tr><tr><td>Red pencil</td><td></td><td></td><td></td></tr><tr><td>Cotton swabs</td><td></td><td></td><td></td></tr><tr><td>Facilities and supplies for participants to wash their hair after the experiment- sink, shampoo, comb, towels, hair dryer</td><td></td><td></td><td></td></tr></tbody></table>

investigating the effects of antipsychotics and schizotypy on the n400 using event-related potentials and semantic categorization

在认知神经科学领域，功能性磁共振成像（fMRI）技术是可视化脑功能的常用方法。这是因为其出色的空间分辨率，这使研究人员能够确定与特定的认知过程相关的脑区的一部分。但是，在追求本地化脑的功能，它是相关的要注意的是许多认知，感觉和运动过程具有时间差别是势在必行捕获，即左未了通过磁共振成像的次优的时间分辨率的一个方面。为了更好地理解认知过程，它是这样有利地利用事件相关电位（ERP）的记录作为收集有关脑的信息的方法。它的一些优点包括它的奇妙的时间分辨率，这使得研究人员能够跟随大脑的活动到毫秒的能力。它也直接索引都兴奋性和抑制性突触后电位由其中​​大部分脑计算执行。这个位于相反的fMRI，其捕获的代谢活性的指标。另外，非侵入性的ERP方法不需要造影条件:原料的ERP可以检查对于只有一个实验条件下，从功能磁共振成像的区分，其中控制的条件必须由实验条件中减去，导致不确定性与实验相关联的观测值或对比条件。虽然它是由它的可怜的空间和皮质下活动分辨率的限制，ERP录音的效用，相对成本效益，以及相关的优势提供了强大的理论基础及其在认知神经科学使用，以追踪神经活动的快速随时间的变化。在努力促进增加其作为研究成像方法的使用，并确保正确和准确的数据采集，本文章将概述 - 在一个范式的框架内使用语义分类研究抗精神病药物的影响s和schizotypy的N400 - 的程序和ERP数据采集相关的关键环节。

五个数字已包括帮助研究人员获得最佳的ERP记录图1描述了成功完成该协议下获得的ERP波形。它构成了"良好"的结果，因为它准确地记录; 图2在另一方面，示出的ERP波形将描绘一个差的执行的协议的，从而产生"坏"的结果，其在噪音过大的信号，最主要的是标识。 图3示出了可能出现在ERP的记录，可以阻碍正确的数据集合中的常见问题。高活性可以解释在高频和大振幅的波。与此相反，一个"平直线"表示缺乏活动或不当的连接。 图4和图5显示了盛大的平均值为我们调查的主要结果纳入对抗精神病药物和schizotypy的影响在N400，如使用语义分类模式进行测定。 <img alt="图1" src="/files/ftp_upload/52082/52082fig1highres.jpg" /> 图1.良好的效果。该图描述了一个主题在所有试验之后成功地执行了该技术的平均ERP波形，造成不平整的线或噪音过大。需要注意的是波看起来略有不同，这取决于其在很大程度上依赖于不同的过程（认知，感觉和运动）将产生根据任务的内容和由该任务所需的技巧不同波形的实验范例使用-的任务。 <a href="https://jove.unicartagenaproxy.elogim.com/files/ftp_upload/52082/52082fig1large.jpg" target="_blank">请点击这里查看该图的放大版本。</a> <img alt="图2" src="/files/ftp_upload/52082/52082fig2highres.jpg" /&gt; 图2.可怜的结果。此图描绘了一个主题在所有试验后的技术执行不足平均ERP波形。此处所示的波形差是由于噪声，以及在FP2的扁平线。说明还缺少在脑电图识别的ERP组件。 <a href="https://jove.unicartagenaproxy.elogim.com/files/ftp_upload/52082/52082fig2large.jpg" target="_blank">请点击这里查看这个数字的放大版本。</a> <img alt="图3" src="/files/ftp_upload/52082/52082fig3highres.jpg" /> 图3.常见问题该图描绘了出现这种技术中普遍存在的问题;即，眼球运动，肌肉运动，或饱和度。需要注意的是，这些问题可以发生在任何通道的，所以一定要检查所有的人。 <a href="https://jove.unicartagenaproxy.elogim.com/files/ftp_upload/52082/52082fig3large.jpg" target="_blank">请点击此处争夺</a>WA更大的版本这个数字。 <img alt="图4" src="/files/ftp_upload/52082/52082fig4highres.jpg" /> Schizotypy图4.影响。该图描述了在安慰剂条件隆重平均的ERP。红色的线对应于参与者高分的分裂型人格问卷。黑线对应的参与者低schizotypy通过问卷调查来确定。 <img alt="图5" src="/files/ftp_upload/52082/52082fig5highres.jpg" /> 图5.药物影响高schizotypy个人，这个数字描绘了宏伟平均的ERP，其中红色对应药物（奥氮平）和黑色对应于安慰剂。 <a href="https://jove.unicartagenaproxy.elogim.com/files/ftp_upload/52082/EEGLab_Tutorial.mp4" target="_blank">请点击这里查看大图VERSI</a>在这个数字的。 动画图1.该图可被用作用于处理数据和获取事件相关电位的指南。

Watch this Scientific Journal Video about Investigating the Effects of Antipsychotics and Schizotypy on the N400 Using Event-Related Potentials and Semantic Categorization at JoVE.com

Investigating the Effects of Antipsychotics and Schizotypy on the N400 Using Event-Related Potentials and Semantic Categorization

采用事件相关脑电位（ERPs），我们研究在健康人与分裂型性状异常的语义激活大脑抗精神病药物的效果。我们使用的ERP来跟踪不同的变化，大脑活动，脱落的洞察与语义分类相关的认知过程。

使用事件相关电位和语义分类调查抗精神病药和Schizotypy在N400的影响

Within the field of cognitive neuroscience, functional magnetic resonance imaging (fMRI) is a popular method of visualizing brain function. This is in ...

investigating-effects-antipsychotics-schizotypy-on-n400-using-event

Universidad de Cartagena UDEC

Research

Education

JoVE Core

JoVE Journal

Pharmacology

Behavior

Unit 1

Pharmacotherapy of Psychosis and Mania

SCIENTISTS IN ACTION

12.8K 次观看.  McGill University. 采用事件相关脑电位（ERPs），我们研究在健康人与分裂型性状异常的语义激活大脑抗精神病药物的效果。我们使用的ERP来跟踪不同的变化，大脑活动，脱落的洞察与语义分类相关的认知过程。 

视频: Investigating the Effects of Antipsychotics and Schizotypy on the N400 Using Event-Related Potentials and Semantic Categorization

P50 Sensory Gating in Infants

Attentional deficits are common in a variety of neuropsychiatric disorders including attention deficit-hyperactivity disorder, autism, bipolar mood disorder, and schizophrenia. There has been increasing interest in the neurodevelopmental components of these attentional deficits; neurodevelopmental meaning that while the deficits become clinically prominent in childhood or adulthood, the deficits are the results of problems in brain development that begin in infancy or even prenatally. Despite this interest, there are few methods for assessing attention very early in infancy. This report focuses on one method, infant auditory P50 sensory gating.
Attention has several components. One of the earliest components of attention, termed sensory gating, allows the brain to tune out repetitive, noninformative sensory information. Auditory P50 sensory gating refers to one task designed to measure sensory gating using changes in EEG. When identical auditory stimuli are presented 500 ms apart, the evoked response (change in the EEG associated with the processing of the click) to the second stimulus is generally reduced relative to the response to the first stimulus (i.e. the response is &#34;gated&#34;). When response to the second stimulus is not reduced, this is considered a poor sensory gating, is reflective of impaired cerebral inhibition, and is correlated with attentional deficits.
Because the auditory P50 sensory gating task is passive, it is of potential utility in the study of young infants and may provide a window into the developmental time course of attentional deficits in a variety of neuropsychiatric disorders. The goal of this presentation is to describe the methodology for assessing infant auditory P50 sensory gating, a methodology adapted from those used in studies of adult populations.

Methods to record auditory P50 sensory gating, a physiological marker of cerebral inhibition which reflects early stages of attention, are described.

P50感觉门控的婴幼儿

Attentional deficits are common in a variety of neuropsychiatric disorders including attention deficit-hyperactivity disorder, autism, bipolar mood ...

科研

行为学

High Density Event-related Potential  Data Acquisition in Cognitive Neuroscience

Functional magnetic resonance imaging (fMRI) is currently the standard method of evaluating brain function in the field of Cognitive Neuroscience, in part because fMRI data acquisition and analysis techniques are readily available. Because fMRI has excellent spatial resolution but poor temporal resolution, this method can only be used to identify the spatial location of brain activity associated with a given cognitive process (and reveals virtually nothing about the time course of brain activity). By contrast, event-related potential (ERP) recording, a method that is used much less frequently than fMRI, has excellent temporal resolution and thus can track rapid temporal modulations in neural activity. Unfortunately, ERPs are under utilized in Cognitive Neuroscience because data acquisition techniques are not readily available and low density ERP recording has poor spatial resolution. In an effort to foster the increased use of ERPs in Cognitive Neuroscience, the present article details key techniques involved in high density ERP data acquisition. Critically, high density ERPs offer the promise of excellent temporal resolution and good spatial resolution (or excellent spatial resolution if coupled with fMRI), which is necessary to capture the spatial-temporal dynamics of human brain function.

Event-related potential (ERP) recording is under utilized in Cognitive Neuroscience because data acquisition techniques are not readily available and this method often has poor spatial resolution. To foster the increased use of ERPs in Cognitive Neuroscience, the present article details key techniques involved in high density ERP data acquisition.

高密度事件相关电位在认知神经科学的数据采集

Functional magnetic resonance imaging (fMRI) is currently the standard method of evaluating brain function in the field of Cognitive Neuroscience, in part ...

神经科学

Methods to Explore the Influence of Top-down Visual Processes on Motor Behavior

Kinesthetic awareness is important to successfully navigate the environment. When we interact with our daily surroundings, some aspects of movement are deliberately planned, while others spontaneously occur below conscious awareness. The deliberate component of this dichotomy has been studied extensively in several contexts, while the spontaneous component remains largely under-explored. Moreover, how perceptual processes modulate these movement classes is still unclear. In particular, a currently debated issue is whether the visuomotor system is governed by the spatial percept produced by a visual illusion or whether it is not affected by the illusion and is governed instead by the veridical percept. Bistable percepts such as 3D depth inversion illusions (DIIs) provide an excellent context to study such interactions and balance, particularly when used in combination with reach-to-grasp movements. In this study, a methodology is developed that uses a DII to clarify the role of top-down processes on motor action, particularly exploring how reaches toward a target on a DII are affected in both deliberate and spontaneous movement domains.

It is unclear how top-down signals from the ventral visual stream affect movement. We developed a paradigm to test motor behavior towards a target on a 3D depth inversion illusion. Significant differences are reported in both deliberate, goal-directed movements and automatic actions under illusory and veridical viewing conditions.

方法探索自上而下视觉过程对电机性能的影响

Kinesthetic awareness is important to successfully navigate the environment. When we interact with our daily surroundings, some aspects of movement are ...

Measurement of Fronto-limbic Activity Using an Emotional Oddball Task in Children with Familial High Risk for Schizophrenia

Adolescence is a critical developmental period where the early symptoms of schizophrenia frequently emerge. First-degree relatives of people with schizophrenia who are at familial high risk (FHR) may show similar cognitive and emotional changes. However, the neurological changes underlying the emergence of these symptoms remain unclear. This study sought to identify differences in frontal, striatal, and limbic regions in children and adolescents with FHR using functional magnetic resonance imaging. Groups of 21 children and adolescents at FHR and 21 healthy controls completed an emotional oddball task that relied on selective attention and the suppression of task-irrelevant emotional information. The standard oddball task was modified to include aversive and neutral distractors in order to examine potential group differences in both emotional and executive processing. This task was designed specifically to allow for children and adolescents to complete by keeping the difficulty and emotional image content age-appropriate. Furthermore, we demonstrate a technique for suitable fMRI registration for children and adolescent participants. This paradigm may also be applied in future studies to measure changes in neural activity in other populations with hypothesized developmental changes in executive and emotional processing.

This paper describes how to use the emotional oddball task and fMRI to measure brain activation in children and adolescents at familial high risk for schizophrenia (FHR).&#160;FMRI was used to measure differences in fronto-striato-limbic regions during an emotional oddball task. Children with FHR exhibited abnormal functional activation during adolescence.

的额边缘活度测量在儿童使用情绪穿着或行为古怪的任务与家族性高风险的精神分裂症

Adolescence is a critical developmental period where the early symptoms of schizophrenia frequently emerge. First-degree relatives of people with ...

A Semantic Priming Event-related Potential (ERP) Task to Study Lexico-semantic and Visuo-semantic Processing in Autism Spectrum Disorder

Individuals with autism spectrum disorder (ASD) have characteristic deficits in understanding the meaning of language, or semantic processing. However, some evidence indicates that semantic processing of non-linguistic stimuli is intact, suggesting that semantic deficits may be language-specific. To appropriately characterize semantic processing deficits in individuals with ASD, comparison of within-modality linguistic (e.g., written words) and non-linguistic (e.g., pictures) stimuli is required. This paper describes such a methodology that makes use of a semantic priming paradigm during concurrent recording of electroencephalographic (EEG) data. EEG provides a dynamic measure of brain activity that is well-suited to characterize subtle differences in semantic processing that may not be observable at the behavioral level. The semantic priming paradigm presents a prime picture or word (e.g., dog) followed by a target picture or word that is either related (e.g., cat) or unrelated (e.g., pencil) to the prime. This paradigm can thus be used to evaluate semantic processing across different modalities, and to compare lexico-semantic and visuo-semantic processing abilities in individuals with ASD and how they might differ from TD individuals. The specific steps involved in creating the stimuli, performing the EEG testing, and analyzing the EEG data are discussed. Representative results illustrate how the N400 component of the event-related potential (ERP) is reduced following semantically-related prime-target pairs compared to unrelated pairs. Comparisons of the N400 between conditions, modalities, and groups can provide estimates of the success of semantic processing, and can thereby be used to characterize semantic deficits in individuals with ASD or other clinical populations.

A Semantic Priming Event-related Potential ERP Task to Study Lexico-semantic and Visuo-semantic Processing in Autism Spectrum Disorder

This paper describes a semantic priming ERP task using within-modality pairs of pictures and words to investigate semantic processing in individuals with autism spectrum disorder (ASD).

语义启动事件相关电位 (ERP) 研究自闭症频谱障碍词汇语义和视觉语义处理

Individuals with autism spectrum disorder (ASD) have characteristic deficits in understanding the meaning of language, or semantic processing. However, ...

Dissociation of the Confounding Influences of Expectancy and Integrative Difficulty Residing in Anomalous Sentences in Event-related Potential Studies

The confounding factors of unexpectedness and semantic integration difficulty naturally residing in anomalous sentences in language studies make it difficult to determine the underlying processing mechanism of ERP components. Unlike the traditional static approach of manipulating expectancy through corpus frequency or cloze probability, this protocol proposes a dynamic method to enhance participants&#39; expectancy for rarely-met anomalous sentences by multiple repetitions while maintaining their semantic integration difficulties. To address the time cost increase resulting from multiple repetitions, this protocol proposes to repeat only the strictly simplified core structure extracted from the anomalous sentence before presenting the semantically enriched, much more informative complete anomalous sentence containing the anomalous core structure to reinitiate the semantic integration processing. The complete anomalous sentence elicited a P600 effect. It suggests that the participants did not give up processing the anomalous information after repetitions and the same semantic integration difficulty was successfully reinitiated. Importantly, the representative experimental results reveal that the greatly attenuated N400 effect caused by multiple repetitions was not recovered by the follow-up reinitiated semantic integration difficulty. It suggests that the attenuated N400 effect should be mainly attributed to the enhancement of expectancy for anomalous information by multiple repetitions. The experimental results show that this method can effectively enhance participants&#39; expectancy for anomalous sentences while retaining the semantic integration difficulty.

We present a protocol to dissociate the intertwining factors of integrative difficulty and unexpectedness in semantically anomalous sentences by applying multiple repetitions to enhance participant&#39;s expectancy for anomalous sentences. The dissociation helps to investigate the major contributor of elicited event-related potentials (ERP) effects such as N400 in language studies.

与事件相关的潜在研究中,在异常句中所驻住的期望值与综合难度的分离

The confounding factors of unexpectedness and semantic integration difficulty naturally residing in anomalous sentences in language studies make it ...

Interaction between Phonological and Semantic Processes in Visual Word Recognition using Electrophysiology

Controversies have always existed in research related to reading abilities; on whether printed words are perceived in a feedforward manner based on orthographic information after which, other representations, such as phonology and semantics are activated, or whether these are fully interactive and high-level semantic information affects early processing. An interference paradigm was implemented in the presented protocol of phonological and semantic judgment tasks that utilized the same precede-target pairs to explore the relative order of phonological and semantic activation. The high- and low-frequency target words were preceded with three conditions: semantically related, phonological-related (homophones), or unrelated. The results showed that the induced P200 component of low-frequency word pairs was significantly greater than high-frequency words in both the semantic and phonological tasks. In addition, both the homophones in the semantic task and the semantically related pairs in the phonological task caused reduction in N400 when compared to the the control condition, word frequency-independently. It is worth noting that for the low-frequency pairs in the phonological judgment task, the P200 released by the semantically related word pairs was significantly larger than that in the control condition. Overall, semantic processing in phonological tasks and phonological processing in semantic tasks were found in both high- and low-frequency words, suggesting that the interaction between semantics and phonology may operate in a task-independent manner. However, the specific time this interaction occurred may have been affected by the task and frequency.

We present a protocol to explore the relative activation sequence of phonology and semantics in visual word recognition. The results show that consistent with interactive accounts, semantic and phonological representations may be processed interactively, and higher-level linguistic representations may affect early processing.

使用电生理学的视觉单词识别中语音和语义过程的相互作用

Controversies have always existed in research related to reading abilities; on whether printed words are perceived in a feedforward manner based on ...

How to Find Effects of Stimulus Processing on Event Related Brain Potentials of Close Others when Hyperscanning Partners

The partners of each pair must be able to pass the McGill Friendship Questionnaire without communicating. Each partner is then seated in front of a screen in one of two adjacent rooms. These rooms are separated by a glass window through which participants communicate to maintain feelings of togetherness while being fitted with the EEG cap. After checking for adequate EEG signals, the glass is covered by a curtain to prevent visual communication. Then, partners must be silent but are instructed to try to feel in the presence of their partner during the entire experiment. Just before it starts, participants are told that each of them will be presented with one image at a time and that these images will occur at the same time for both of them on their own screen. They are also instructed that, for each trial, the simultaneous images will always be different. However, unbeknownst to them, trials are randomized: only half of them are consistent with this instruction and actually include two different images. These trials form the DSC, that is, the different-stimuli condition. The other half of the trials are inconsistent with the instruction. They include two identical images and form the ISC (identical-stimuli condition). After the experiment, participants are sorted into two groups: those who reported that they felt in the presence of their partner during the majority of the trials and those who reported they did not. The impact of the stimulus processing of the partner is found by subtracting the mean voltages of the ERPs of the ISC (inconsistent with the instructions) from the ERPs of the DSC (consistent with the instructions) in at least two time windows (TWs): firstly, in the 75 to 150 ms TW, where the absolute values of these subtractions are greater, especially at right frontal sites, in those who felt in the presence of their partner than in those who did not; secondly, in the LPP time window (i.e., from 650 to 950 ms post onset), where ERPs are significantly less positive in the DSC than in the ISC in those in whom the raw results of the early (75-150ms) subtractions are negative.

This protocol describes key steps involved in assessing the sensitivity of the brain of one person to the stimulus processing of a close other by selecting pairs of partners, recording their electroencephalogram (EEG) simultaneously and computing their event-related brain potentials (ERPs).

Hyperscanning 合作伙伴时如何发现刺激处理对近距离活动相关脑电位的影响

The partners of each pair must be able to pass the McGill Friendship Questionnaire without communicating. Each partner is then seated in front of a screen ...

Detecting Pre-Stimulus Source-Level Effects on Object Perception with Magnetoencephalography

Pre-stimulus oscillatory brain activity influences upcoming perception. The characteristics of this pre-stimulus activity can predict whether a near-threshold stimulus will be perceived or not perceived, but can they also predict which one of two competing stimuli with different perceptual contents is perceived? Ambiguous visual stimuli, which can be seen in one of two possible ways at a time, are ideally suited to investigate this question. Magnetoencephalography (MEG) is a neurophysiological measurement technique that records magnetic signals emitted as a result of brain activity. The millisecond temporal resolution of MEG allows for a characterization of oscillatory brain states from as little as 1 second of recorded data. Presenting an empty screen around 1 second prior to the ambiguous stimulus onset therefore provides a time window in which one can investigate whether pre-stimulus oscillatory activity biases the content of upcoming perception, as indicated by participants&#39;&#160;reports. The spatial resolution of MEG is not excellent, but sufficient to localise sources of brain activity at the centimetre scale. Source reconstruction of MEG activity then allows for testing hypotheses about the oscillatory activity of specific regions of interest, as well as the time- and frequency-resolved connectivity between regions of interest. The described protocol enables a better understanding of the influence of spontaneous, ongoing brain activity on visual perception.

This article describes how to set up an experiment that allows detecting pre-stimulus source-level influences on object perception using magnetoencephalography (MEG). It covers stimulus material, experimental design, MEG recording, and data analysis.

利用磁脑学检测刺激前源级对物体感知的影响

Pre-stimulus oscillatory brain activity influences upcoming perception. The characteristics of this pre-stimulus activity can predict whether a ...

The Joint Effect of Social Comparison and Social Distance on Evaluation of Intertemporal Choice Outcomes in Event-related Potential Studies

Intertemporal choice plays a crucial role in our daily lives, influencing decisions related to education, health, consumption, and investment. This research proposes an innovative experimental protocol that examines how social comparison and social distance jointly affect the neural processes involved in outcome assessment for intertemporal choices. The study is based on the theoretical framework of cognitive resource competition. This protocol enables researchers to dynamically establish an indifference point for each participant, effectively eliminating the influence of any biased indifference points on the assessment of intertemporal choices. Consequently, the study solely measures the combined impact of social comparison and social distance on how participants evaluate intertemporal choice outcomes. The findings reveal that individuals are more inclined to opt for immediate outcomes under negative unfair conditions. Moreover, compared to the fair and positive unfair conditions, people tend to undervalue delayed outcomes in the negative unfair condition. The strength of this approach lies in its dynamic indifference point setting, making it an effective method to investigate the influence of various external factors (such as social status and power level) on intertemporal decision-making. While the protocol is designed to measure electrophysiological events like event-related potentials, it can also be tailored for use with fMRI.

This protocol aims to investigate the neural activity related to social comparison and social distance during the processing of intertemporal decision outcomes. Indifference points will be measured using event-related potentials as part of the study.

社会比较与社会距离对事件相关潜力研究中跨期选择结局评价的联合影响

Intertemporal choice plays a crucial role in our daily lives, influencing decisions related to education, health, consumption, and investment. This ...