Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
5.7K Views
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08:21 min
•
January 14th, 2021
DOI :
January 14th, 2021
•Yu-Han Yeh*1,2, Mengwen Hu*1,2, Toshinori Nakagawa3,4, Akihiko Sakashita1,2, Shosei Yoshida3,4, So Maezawa5,6, Satoshi H. Namekawa1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine, 3Division of Germ Cell Biology, National Institute for Basic Biology, National Institutes of Natural Sciences, 4Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies (Sokendai), 5Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, 6Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science
Chapters in this video
0:04
Introduction
0:53
Testicular Cell Suspension Preparation
2:35
Cell Staining
3:30
Flow Cytometry
7:08
Results: Representative Spermatogenic Cell Imaging, Yield, and Viability
7:55
Conclusion
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