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Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species

6.8K Views

07:37 min

February 21st, 2020

DOI :

10.3791/60868-v

February 21st, 2020


Explore More Videos

Multiplex Immunostaining

Chapters in this video

0:05

Introduction

0:42

First Target Protein Staining

3:15

Antibody Stripping

3:41

Second Target Protein Staining

4:49

Fluorescence Microscopy Imaging

5:30

Results: Representative Effects of Microwave-Mediated Stripping on Antibody Cross-Reactivity

6:38

Conclusion

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