Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples
2.0K Views
•
05:26 min
•
February 3rd, 2023
DOI :
February 3rd, 2023
•Roberto Plebani1,2, Alessandra D'Alessandro2, Paola Lanuti2,3, Pasquale Simeone2,3, Massimiliano Cinalli1, Ilaria Righi4, Alessandro Palleschi4,5, Matteo Mucci1,2, Marco Marchisio2,3, Francesco Cappabianca1, Marina Camera6,7, Felice Mucilli1, Mario Romano1,2
1Department of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 2Center on Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara, 3Department of Medicine and Aging Sciences, University “G. d’Annunzio” of Chieti-Pescara, 4U.O. di Chirurgia Toracica e dei Trapianti di Polmone, Fondazione IRCCS Ca’ Granda - Ospedale Maggiore Policlinico di Milano, 5Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti, Università degli Studi di Milano, 6Department of Pharmaceutical Sciences, Università degli Studi di Milano, 7Unit of Cell and Molecular Biology in Cardiovascular Diseases, Centro Cardiologico Monzino IRCCS
Chapters in this video
0:04
Introduction
0:45
Sample Preparation and Enzymatic Digestion
1:47
Recovery of the Digested Cells and Removal of Non-Endothelial Cells (EC) by Filtration
2:32
Sedimentation of Cell Clusters and Cell Seeding
3:16
Results: Purification and Characterization of Freshly Isolated HLMVECs
4:30
Conclusion
Related Videos
10:54
Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol
15.2K Views
09:04
Isolation of Mouse Lung Dendritic Cells
29.0K Views
08:14
Isolation of Primary Murine Brain Microvascular Endothelial Cells
32.2K Views
10:46
Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice
47.0K Views
09:23
Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries
15.6K Views
06:41
Isolation of Primary Mouse Lung Endothelial Cells
5.0K Views
08:42
Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
2.6K Views
07:36
Isolation of Mouse Coronary Endothelial Cells
11.1K Views
11:49
Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
8.0K Views
09:12
Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments
3.1K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved