Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells
1.7K Views
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12:54 min
•
May 19th, 2023
DOI :
May 19th, 2023
•Fatima Liaqat1, Richard Thiga Kangethe1, Rudolf Pichler1, Bo Liu1, Johann Huber2, Viskam Wijewardana1, Giovanni Cattoli1, Luca Porfiri1
1Animal Production and Health Section, Joint Food and Agriculture Organization (FAO)/International Atomic Energy Agency (IAEA) Centre of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, 2Teaching and Research Farm Kremesberg, Clinical Unit for Herd Health Management in Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna
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Chapters in this video
0:04
Introduction
1:09
Production of Naive Bovine Monocyte-Derived Dendritic Cells (MoDCs)
6:01
Generation of Antigen-Pulsed MoDCs
7:20
MoDC Lymphocyte Co-Culture
8:20
Flow Cytometric Analysis
9:47
Results: In Vitro Generation of Cattle MoDCs for the Evaluation of Candidate Vaccine Antigens
12:10
Conclusion
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