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Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana

366 Views

11:48 min

March 17th, 2023

DOI :

10.3791/64817-v

March 17th, 2023


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Chromatin Immunoprecipitation

Chapters in this video

0:00

Introduction

0:30

Protein‑DNA Cross‑Linking

2:13

Homogenization and Lysis

3:30

Sonication

4:08

Immunoprecipitation (IP)

5:28

Recovery of the Immunocomplexes with Magnetic Beads

6:20

Washing, Elution, and Cross‑Linking Reversal

8:13

Protein and RNA Digestion and DNA Purification

10:07

Results: The Specificity and Effectiveness of the Assay by Quantitative PCR and Next‑Generation Sequencing

11:28

Conclusion

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