Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana
370 Views
•
11:48 min
•
March 17th, 2023
DOI :
March 17th, 2023
•Mascha Fiona Dix*1, Peng Liu*2, Guoxin Cui1, Francesco Della Valle2, Valerio Orlando2, Manuel Aranda1
1Red Sea Research Center (RSRC), Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), 2KAUST Environmental Epigenetics Program (KEEP), Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST)
Chapters in this video
0:00
Introduction
0:30
Protein‑DNA Cross‑Linking
2:13
Homogenization and Lysis
3:30
Sonication
4:08
Immunoprecipitation (IP)
5:28
Recovery of the Immunocomplexes with Magnetic Beads
6:20
Washing, Elution, and Cross‑Linking Reversal
8:13
Protein and RNA Digestion and DNA Purification
10:07
Results: The Specificity and Effectiveness of the Assay by Quantitative PCR and Next‑Generation Sequencing
11:28
Conclusion
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