Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
1.5K Views
•
08:18 min
•
April 7th, 2023
DOI :
April 7th, 2023
•Chapters in this video
0:04
Introduction
1:01
Oligonucleotide‐Conjugated Antibody Staining
4:39
Multiplexed Imaging Reporter Plate
5:16
Operating the Multiplexed Imaging Machine
6:31
Multiplex Image Collection and Analysis
7:01
Results: Visualization of the Acquired Multiplexed Images
7:28
Conclusion
Related Videos
07:58
Cell-Free DNA Integrity Analysis in Urine Samples
13.7K Views
10:12
Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
8.8K Views
11:20
Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology
10.8K Views
06:36
Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue
6.9K Views
08:38
Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
21.1K Views
06:07
Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells
5.5K Views
09:58
Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
2.7K Views
08:32
Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model
6.0K Views
09:17
Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
2.2K Views
06:47
Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
2.1K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved