A Mouse Model for the Transition of Streptococcus pneumoniae from Colonizer to Pathogen upon Viral Co-Infection Recapitulates Age-Exacerbated Illness
2.3K Views
•
12:21 min
•
September 28th, 2022
DOI :
September 28th, 2022
•Alexsandra Lenhard*1, Basma H. Joma*2,3, Nalat Siwapornchai2, Anders P. Hakansson4, John M. Leong2,5, Elsa N. Bou Ghanem1
1Department of Microbiology and Immunology, University at Buffalo School of Medicine, 2Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 3Graduate Program in Immunology, Tufts Graduate School of Biomedical Sciences, 4Department of Translational Medicine, Lund University, 5Stuart B. Levy Center for the Integrated Management of Antimicrobial Resistance, Tufts University
Chapters in this video
0:05
Introduction
0:55
Growing Streptococcus pneumoniae Biofilm
4:15
Intranasal Inoculation of Mice with Biofilm-Grown S. pneumoniae
5:11
Viral Infection with Influenza A Virus (IAV)
6:01
Processing of Infected Tissues for Bacterial Enumeration
8:35
Processing of the Lung Samples for Flow Cytometry
9:07
Results: Enhancement of S. pneumoniae Pathogenicity upon Viral Infection and Advanced Age
11:43
Conclusion
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