A High-Throughput Platform for Culture and 3D Imaging of Organoids
2.4K Views
•
07:42 min
•
October 14th, 2022
DOI :
October 14th, 2022
•Gianluca Grenci1,2, Florian Dilasser1, Saburnisha Binte Mohamad Raffi1, Marion Marchand1, Mona Suryana1, Geetika Sahni1, Virgile Viasnoff1,3,4, Anne Beghin1,5
1Mechanobiology Institute (MBI), National University of Singapore, 2Biomedical Engineering Department, National University of Singapore, 3Department of Biological Sciences, National University of Singapore, 4IRL 3639 CNRS, 5Department of Microbiology & Immunology, Immunology Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore
Chapters in this video
0:04
Introduction
0:49
PDMS Mold Dicing and Production of the UV‐Curable, Adhesive, Textured Layer
2:19
Coverslip Substrate Preparation
3:34
Transferring the Textured Film to the Final Substrate
4:33
Cell Seeding, Release, and Collection of the Organoids
6:29
Results: Fabrication of the Microwell Culture Dish for a High‐Density Organoid Culture and Differentiation
7:00
Conclusion
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