Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
2.4K Views
•
09:09 min
•
August 17th, 2022
DOI :
August 17th, 2022
•Brieuc Chauvin*1, Koyomi Nakazawa*1, Alexandre Beber1,7, Aurélie Di Cicco1, Bassam Hajj1, François Iv2, Manos Mavrakis2, Gijsje H. Koenderink3, João T. Cabral4, Michaël Trichet5, Stéphanie Mangenot*6, Aurélie Bertin*1
1Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, 2Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 3Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, 4Department of Chemical Engineering, Imperial College London, 5Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Service de microscopie électronique (IBPS-SME), 6Laboratoire Matière et Systèmes Complexes (MSC), Université Paris Cité, 7Institute of Biotechnology, Czech Academy of Sciences, BIOCEV
Explore More Videos
Chapters in this video
0:05
Introduction
0:49
Designing Wavy NOA (Norland Optical Adhesive) Replica from Wavy PDMS (Polydimethylsiloxane) Patterns
1:49
Generation of a Supported Lipid Bilayer and Protein Incubation
3:22
Sample Fixation, Sample Dehydration, Critical Point Drying
4:43
Sample Mounting and Coating
5:54
Acquire the Images Using a Field-Emission SEM (FE-SEM) Microscope
6:56
Results: Analysis of the Self-Assembly of Septins at the Lipid Membrane
8:27
Conclusion
Related Videos
12:30
Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards
11.4K Views
08:38
Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
22.0K Views
14:23
Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome
18.6K Views
08:14
Who is Who? Non-invasive Methods to Individually Sex and Mark Altricial Chicks
18.3K Views
10:01
In vitro Methylation Assay to Study Protein Arginine Methylation
15.1K Views
12:48
Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery
9.7K Views
11:02
Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
8.0K Views
11:02
Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
28.8K Views
05:58
Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization
3.0K Views
07:04
Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies
2.3K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved