Intravital Widefield Fluorescence Microscopy of Pulmonary Microcirculation in Experimental Acute Lung Injury Using a Vacuum-Stabilized Imaging System
2.8K Views
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09:28 min
•
April 6th, 2022
DOI :
April 6th, 2022
•Stefan Hall1, Sufyan Faridi2, Irene Euodia2,3, Sophie Tanner4, Andrew Krzysztof Chojnacki5, Kamala D. Patel6,7, Juan Zhou2,4, Christian Lehmann1,2,4,8
1Department of Physiology and Biophysics, Dalhousie University, 2Department of Microbiology and Immunology, Dalhousie University, 3Department of Neuroscience, Dalhousie University, 4Department of Anesthesia, Pain Management, and Perioperative Medicine, Dalhousie University, 5Live Cell Imaging Center, University of Calgary, 6Department of Physiology and Pharmacology, University of Calgary, 7Department of Biochemistry and Molecular Biology, University of Calgary, 8Department of Pharmacology, Dalhousie University
Chapters in this video
0:04
Introduction
0:36
Preparation of the Vacuum-Stabilized Lung Imaging System
1:10
Intubation
1:57
Ventilation
2:47
Thoracotomy
5:10
Microscopy
6:56
Results: Leukocyte Trafficking and Capillary Perfusion in the Pulmonary Microcirculation
8:48
Conclusion
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