Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow
4.0K Views
•
07:48 min
•
April 1st, 2022
DOI :
April 1st, 2022
•Mohammad N. Qasim*1,2, Ashley Valle Arevalo*1,2, Akshay D. Paropkari*1,2, Craig L. Ennis1,2,4, Suzanne S. Sindi3, Clarissa J. Nobile1,4, Aaron D. Hernday1,4
1Department of Molecular and Cell Biology, University of California, Merced, 2Quantitative and Systems Biology Graduate Program, University of California, Merced, 3Department of Applied Mathematics, University of California, Merced, 4Health Sciences Research Institute, University of California, Merced
Explore More Videos
Chapters in this video
0:04
Introduction
0:49
Isolation of Nuclei and Primary Antibody Binding
2:40
Binding of pAG-MNase to Antibody
3:36
Library Preparation for Sequencing
5:22
CUT&RUN Sequence Analysis
5:54
Results: Quality Control Steps and Evaluation of Ndt80 and Efg1 Enriched Peaks Using CUT&RUN Protocol
7:22
Conclusion
Related Videos
14:42
Isolation and Analysis of Hematopoietic Stem Cells from the Placenta
20.3K Views
07:32
Genome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays
12.4K Views
11:19
Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
14.4K Views
11:39
A Quantitative Fitness Analysis Workflow
14.4K Views
12:39
A Novel Bayesian Change-point Algorithm for Genome-wide Analysis of Diverse ChIPseq Data Types
11.3K Views
14:07
Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
16.5K Views
10:56
Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
68.2K Views
10:05
Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)
8.2K Views
09:14
DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
19.3K Views
11:02
Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
28.8K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved