Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
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08:26 min
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April 6th, 2022
DOI :
April 6th, 2022
•Nadezhda Podoplelova1,2, Polina Soloveva1,4, Andrei Garzon Dasgupta1,2,3, Aleksandra Filkova1,2, Mikhail Panteleev1,2,3,4
1Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, 2National Medical Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, 3Faculty of Physics, Lomonosov Moscow State University, 4Faculty of Biological and Medical Physics, Moscow Institute of Physics and Technology
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Chapters in this video
0:04
Introduction
0:58
Kinetic Binding Experiments
2:13
Equilibrium Binding Experiments
2:45
Analysis of Flow Cytometry Data
5:13
Converting Fluorescence Intensity to the Mean Number of Binding Sites
7:05
Results: Specific Binding of Fluorescent-Labeled Coagulation Factor X or fX to Artificial Phospholipid Vesicles
7:42
Conclusion
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