Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
2.2K Views
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07:35 min
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December 16th, 2021
DOI :
December 16th, 2021
•Maximilian Wulf1,2, Katalin Barkovits-Boeddinghaus1,2, Paula Sommer1,2, Karin Schork1,2, Martin Eisenacher1,2, Peter Riederer3,4, Manfred Gerlach5, Steffen Kösters1,2, Britta Eggers*1,2, Katrin Marcus*1,2
1Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI), Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy, University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research, University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, University Hospital of Wuerzburg, University of Wuerzburg
Chapters in this video
0:05
Introduction
0:46
Laser Microdissection and Pressure Catapulting
2:46
Sample Preparation for HPLC-MS and Proteomic Raw Data Analysis
5:10
Statistical Analysis
5:50
Results: Protein Identification in Neuromelanin Granules (NMGs) and Substantia nigra (SN)
7:00
Conclusion
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