Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy
1.3K Views
•
14:56 min
•
April 21st, 2023
DOI :
April 21st, 2023
•Chapters in this video
0:03
Introduction
1:17
Krebs-Henseleit Buffer Preparation
1:45
Perfusion System Preparation
2:29
Calibration and Preparation of the NMR Spectrometer for Acquisition
3:22
Animal Preparation, Surgical Procedure, and Perfusion of the Heart in the NMR Tube
9:23
Acquiring Data for Cardiac Energetics and pH
10:09
DNP Spin Polarization and Dissolution
11:25
Hyperpolarized 13C Spectroscopy
12:07
Representative Results
14:40
Conclusion
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