Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe
3.8K Views
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09:31 min
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September 17th, 2021
DOI :
September 17th, 2021
•1Molecular Microbial Pathogenesis Unit, Institut Pasteur, 2Chaire de Microbiologie et Maladies Infectieuses, Collège de France, 3Institut de Biologie Paris Seine (IBPS) - Developmental Biology Unit, Sorbonne Université, CNRS UMR7622, INSERM U1156, 4Molecular Radiotherapy, INSERM U1030, Gustave Roussy, Université Paris-Saclay, 5The Center for Microbes, Development and Health, Institut Pasteur Shanghai and Chinese Academy of Sciences, 6Microenvironment and Immunity Unit, Institut Pasteur
Chapters in this video
0:05
Introduction
1:07
Intestinal Organoids Culture
4:13
Visualization of Oxidative Stress in 3D Organoids by Confocal Microscopy
5:42
Quantification of the Oxidative Stress on the Dissociated Organoids Using Flow Cytometer
7:19
Results: Detection of ROS in the Intestinal Murine Organoids Using Qualitative Imaging and Quantitative Cytometry Assays
8:36
Conclusion
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