Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice
2.7K Views
•
08:31 min
•
June 30th, 2021
DOI :
June 30th, 2021
•Corinne M. Nielsen*1,2,3, Yanrong Qian*4, Subhodip Adhicary1,5, Yunsheng Li4, Pratik Shriwas1,2,4, Xuan Wang1,2,4, Lindsey Bachmann6, Xiaozhuo Chen1,2,4,7
1Department of Biological Sciences, Ohio University, 2Molecular and Cellular Biology Program, Ohio University, 3Neuroscience Program, Ohio University, 4Edison Biotechnology Institute, Ohio University, 5Translational Biomedical Sciences Program, Ohio University, 6Honors Tutorial College, Ohio University, 7Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University
Chapters in this video
0:05
Introduction
1:05
Tumor Cell Culture and Xenograft Tumor Development
2:41
HMWFD and NHF-ATP Solution Preparation
3:21
ATP Internalization in Tumor Xenograft
3:53
Cryo-embedding
4:45
Tissue Slide Fixation
5:25
Fluorescence Microscopy and Image Acquisition
7:12
Results: Assessment of Nonhydrolyzable Fluorescent ATP Internalization
7:44
Conclusion
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