Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor
3.5K Views
•
07:59 min
•
June 29th, 2021
DOI :
June 29th, 2021
•Chapters in this video
0:05
Introduction
0:53
Stress Treatment of E. coli Strains MG1655 and CFT073
2:12
Collecting Bacterial Cell Samples
2:43
Extracting the Insoluble Protein Aggregates
4:19
Soluble Protein Sample Preparation
5:30
Separation and Visualization of Extracted Protein Aggregates Using SDS-PAGE
6:49
Results: Visualization of Protein Aggregates After Treatment of Escherichia coli with a Proteotoxic Stressor
7:32
Conclusion
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