Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
4.3K Views
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14:56 min
•
May 6th, 2022
DOI :
May 6th, 2022
•Natália Yumi Noronha*1, Guilherme da Silva Rodrigues*1, Marcela Augusta de Souza Pinhel1, Jean-Baptiste Cazier2,3, Lígia Moriguchi Watanabe1, Albert Nobre Menezes4, Carlos Roberto Bueno5, Carolina Ferreira Nicoletti1, Bruno Affonso Parenti de Oliveira1, Isabelle Mello Schineider6, Isabella Harumi Yonehara Noma7, Igor Caetano Dias Alcarás8, Fernando Barbosa9, Carla Barbosa Nonino6
1Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, 2Centre for Computational Biology, University of Birmingham, 3Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, 4Cancer Genetics and Evolution Laboratory, Cancer Research UK, Institute of Genetics & Molecular Medicine, The University of Edinburgh, 5Ribeirão Preto School of Physical Education and Sport, University of São Paulo, 6Health Sciences Department, Ribeirao Preto Medical School, University of Sao Paulo, 7Faculty of Pharmaceutical Sciences, University of São Paulo, 8Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, 9Department of Clinical, Bromatological and Toxicological Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo
Chapters in this video
0:03
Introduction
0:43
Day 1: Bisulfite Treatment
1:21
Day 2: Amplification - Methylation Assay, Manual Protocol
2:52
Day 3: Hybridization - Methylation Assay, Manual Protocol
6:39
Day 4: Staining - Methylation Assay, Manual Protocol
10:48
Representative Results
14:34
Conclusion
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