Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line
5.4K Views
•
11:02 min
•
February 8th, 2022
DOI :
February 8th, 2022
•1Department of Pathology, Immunology and Laboratory Medicine, Rutgers-New Jersey Medical School, 2Department of Microbiology, Biochemistry & Molecular Genetics, Public Health Research Institute Center, New Jersey Medical School, Rutgers University, 3Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey
Chapters in this video
0:04
introduction
0:54
Natural Killer (NK) Cell Expansion from Liver Tissues: Day 0
3:14
Primary Natural Killer (NK) Cell Expansion from Peripheral Blood Mononuclear Cells (PBMCs): Day 0
4:34
Attachment of 293T Cells (Day 2) and Retrovirus Transfection (Day 3)
5:58
Retronectin Plate-Coating (Day 3) and Transduction (Day 4)
8:18
CAR-NK Cells Collection (Day 6 or 7)
9:04
Results: Expansion of PBNK Cells and Flow Cytometric Analysis of CAR-NK Cells
10:22
Conclusion
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