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Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography

3.2K Views

09:34 min

February 17th, 2022

DOI :

10.3791/62334-v

February 17th, 2022


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Chimeric Antigen Receptor

Chapters in this video

0:04

Introduction

1:02

Ex Vivo T Cell Isolation from Peripheral Blood Mononuclear Cells (PBMCs)

2:47

T Cell Stimulation and T Cell Expansion

3:54

Transduction of Lentiviruses on Stimulated T Cells

5:10

Surface Staining of B Cell Maturation Antigen (BCMA)-CAR Using Goat Anti-Mouse IgG (H+L) and Intracellular Staining of NIS Using Anti-Human ETNL NIS

7:18

NIS+BCMA-CAR T Cell In Vivo Imaging Using BCMA+OPM-2 Xenograft Mouse Model

7:57

Results: T Cell Growth Kinetics and In Vivo Cell Trafficking Assay

9:08

Conclusion

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