Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography
3.2K Views
•
09:34 min
•
February 17th, 2022
DOI :
February 17th, 2022
•Rozdziały w tym wideo
0:04
Introduction
1:02
Ex Vivo T Cell Isolation from Peripheral Blood Mononuclear Cells (PBMCs)
2:47
T Cell Stimulation and T Cell Expansion
3:54
Transduction of Lentiviruses on Stimulated T Cells
5:10
Surface Staining of B Cell Maturation Antigen (BCMA)-CAR Using Goat Anti-Mouse IgG (H+L) and Intracellular Staining of NIS Using Anti-Human ETNL NIS
7:18
NIS+BCMA-CAR T Cell In Vivo Imaging Using BCMA+OPM-2 Xenograft Mouse Model
7:57
Results: T Cell Growth Kinetics and In Vivo Cell Trafficking Assay
9:08
Conclusion
Powiązane Filmy
Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer
17.5K Views
Multimodal Bioluminescent and Positronic-emission Tomography/Computational Tomography Imaging of Multiple Myeloma Bone Marrow Xenografts in NOG Mice
6.7K Views
In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
11.5K Views
Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy
14.8K Views
Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells
10.3K Views
Management of Respiratory Motion Artefacts in 18F-fluorodeoxyglucose Positron Emission Tomography using an Amplitude-Based Optimal Respiratory Gating Algorithm
5.5K Views
Positron Emission Tomography-based Dose Painting Radiation Therapy in a Glioblastoma Rat Model using the Small Animal Radiation Research Platform
2.7K Views
Assessment of Chimeric Antigen Receptor T Cell-Associated Toxicities Using an Acute Lymphoblastic Leukemia Patient-Derived Xenograft Mouse Model
1.2K Views
Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor (CAR) T Cell Infusions in Mice
2.8K Views
Rapid In Vitro Cytotoxicity Evaluation of Jurkat Expressing Chimeric Antigen Receptor using Fluorescent Imaging
2.0K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone