Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block

4.3K Views

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06:10 min

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April 8th, 2021

DOI :

10.3791/62321-v

April 8th, 2021

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Natàlia de Martín Garrido¹, Kailash Ramlaul¹, Christopher H. S. Aylett¹

¹Section of Structural & Synthetic Biology, Department of Infectious Diseases, Faculty of Medicine, Imperial College London, South Kensington Campus

Transcript

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Summary

Explore More Videos

Transmission Electron Microscopy

Cryo EM Samples

Flotation Block

Sample Preparation

Hydrophilic Treatment

Bolting Devices

Negative Staining

Graphene Oxide Suspension

Air Water Interface

Safety Precautions

Chapters in this video

0:04

Introduction

0:47

Preparation of Negatively Stained Samples with Amorphous Carbon Support

2:21

Preparation of Graphene Oxide-coated TEM Grids

3:27

Preparation of Samples on Monolayer-graphene Films Using the Flotation Block

4:44

Results: Examples of Sample Support Films Prepared Using the Floatation Block

5:34

Conclusion

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