Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
3.6K Views
ā¢
10:03 min
ā¢
January 30th, 2021
DOI :
January 30th, 2021
ā¢1Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, 2Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, 3Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine Baltimore, 4Department of Pathology, Albert Einstein College of Medicine, 5Department of Pediatrics, Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina
Chapters in this video
0:04
Introduction
0:27
Isolation and Culture of Human Keratinocytes from Neonatal Foreskin Tissue
2:37
Generating Skin Epidermosphere Cultures in Vitro
5:02
Epidermal Spheroid Re-Plating Assay
5:42
Characterization of Spheroid-Derived (SD) Sub-Populations by FACS
8:06
Results: Characterization of Epidermal Spheroid Formation and Spheroid-Derived NHKs
9:30
Conclusion
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