JoVE Logo

Sign In

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

4.2K Views

10:08 min

May 21st, 2021

DOI :

10.3791/62075-v

May 21st, 2021


Explore More Videos

High throughput Protein Purification

Chapters in this video

0:00

Introduction

0:58

Ni-NTA Chromatography- Purification of 24 Different Proteins: Preparing the Purification Set-up

3:00

Ni-NTA Chromatography- Purification of 24 Different Proteins: Loading, Washing and Eluting

4:47

Ion Exchange- Simultaneous Purification of 6 Different Proteins: Preparing the Purification Set-up

6:15

Ion Exchange- Simultaneous Purification of 6 Different Proteins: Loading, Washing and Eluting

8:25

Results: Representative UV Spectroscopy and SDS PAGE Analyses

Related Videos

article

11:30

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

14.9K Views

article

06:53

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

19.9K Views

article

07:19

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

1.5K Views

article

10:21

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

43.5K Views

article

10:32

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

17.0K Views

article

07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

14.2K Views

article

13:51

The MultiBac Protein Complex Production Platform at the EMBL

15.9K Views

article

10:22

Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis

30.5K Views

article

01:59

Anion-Exchange Chromatography-Based Protein Purification: A Separation Technique to Isolate a Protein of Interest From Dialyzed Bacterial Lysate Based on Net Charge

1.3K Views

article

02:23

Nickel Affinity Chromatography-Based Protein Purification: A Technique to Purify Polyhistidine-Tagged Recombinant Proteins from Bacterial Cell Lysate

4.4K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved