Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System
1.9K Views
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07:04 min
•
February 4th, 2021
DOI :
February 4th, 2021
•Sandra Johnen1, Nina Harmening2,3, Corinne Marie4,5, Daniel Scherman4, Zsuzsanna Izsvák6, Zoltán Ivics7, Peter Walter1, Gabriele Thumann2,3
1Department of Ophthalmology, University Hospital RWTH Aachen, 2Experimental Ophthalmology, University of Geneva, 3Department of Ophthalmology, University Hospitals of Geneva, 4Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé, 5Chimie ParisTech, PSL Research University, 6Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 7Division of Medical Biotechnology, Paul-Ehrlich-Institute
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Chapters in this video
0:05
Introduction
0:57
Primary Human RPE Cell Electroporation and Plasmid DNA Preparation
1:57
Cultivated Primary Human RPE Cell Preparation
3:19
His-Tagged Pigment Epithelium-Derived Factor (PEDF) Fusion Protein Purification
4:57
Results: Representative Primary Human RPE Transfection and Genetic Modification Evaluation
6:24
Conclusion
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