Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current
3.0K Views
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06:22 min
•
November 3rd, 2020
DOI :
November 3rd, 2020
•Philipp Tomsits*1,2,3, Dominik Schüttler*1,2,3, Stefan Kääb1,2, Sebastian Clauss*1,2,3, Niels Voigt*4,5,6
1Department of Medicine I, University Hospital Munich, Campus Großhadern, Ludwig-Maximilians University Munich (LMU), 2Partner Site Munich, Munich Heart Alliance (MHA), DZHK (German Centre for Cardiovascular Research), 3Walter Brendel Center of Experimental Medicine, Ludwig-Maximilians University Munich (LMU), 4Institute of Pharmacology and Toxicology, University Medical Center Göttingen, 5Partner Site Göttingen, DZHK (German Centre for Cardiovascular Research), 6Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Göttingen
Chapters in this video
0:05
Introduction
0:51
Organ Harvest
1:33
Enzymatic Digestion
3:59
Calcium Reintroduction
4:57
Results: Simultaneous Measurement of Calcium Currents and Transients in Atrial and Ventricular Murine Myocytes
5:36
Conclusion
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