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Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

2.7K Views

07:24 min

October 29th, 2020

DOI :

10.3791/61843-v

October 29th, 2020


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Single Cell Multiplexed Fluorescence Imaging

Chapters in this video

0:04

Introduction

0:24

Cell Seeding, Fixation and Permeabilization

1:40

Coverslip Immobilization and Protease Treatment

2:31

Target Probe and Amplifier Hybridization

3:40

Protein Immunostaining and Nuclear Counterstaining

4:48

Mounting

5:28

Results: Representative Single‐Cell Multiplexed Fluorescence Imaging

6:54

Conclusion

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