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PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

4.2K Views

09:31 min

September 26th, 2020

DOI :

10.3791/61839-v

September 26th, 2020


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PCR Mutagenesis

Chapters in this video

0:04

Introduction

0:44

Purification of Wild Type or Mutant Form of α2β2 StTS Complex

3:53

Optimized Crystallization for Wild Type and Mutant Form of the α2β2 StTS Comple

6:49

Results: Purification of the α- and β -Subunits of the Tryptophan Synthase and the Wild Type and Mutant α2β2 StTS Complexes

8:50

Conclusion

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