Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
4.5K Views
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07:10 min
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March 4th, 2021
DOI :
March 4th, 2021
•Mowei Zhou1, Shadan H. Abdali1, David Dilworth2, Lifeng Liu2, Benjamin Cole2, Neha Malhan1, Amir H. Ahkami1, Tanya E. Winkler1, Joy Hollingsworth3, Julie Sievert3, Jeff Dahlberg3, Robert Hutmacher4,5, Mary Madera6, Judith A. Owiti6, Kim K. Hixson1, Peggy G. Lemaux6, Christer Jansson1, Ljiljana Paša-Tolić1
1Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 2DOE-Joint Genome Institute, Lawrence Berkeley Laboratory, 3Kearney Agricultural Research and Extension Center, University of California Agriculture and Natural Resources, 4West Side Research and Extension Center, University of California, 5Department of Plant Sciences, University of California, Davis, 6Department of Plant and Microbial Biology, University of California, Berkeley
Chapters in this video
0:05
Introduction
0:40
Nuclei Isolation from Sorghum Leaf Sample
2:13
Isolation of Nuclei from Remaining Cytoplasmic Contaminants
3:05
Nuclei Lysis and Histone Extraction
4:39
Results: Qualitative & Quantitative Analysis of the Identified Histones
6:36
Conclusion
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