Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
8.0K Views
•
11:49 min
•
July 21st, 2020
DOI :
July 21st, 2020
•Chapters in this video
0:04
Introduction
1:04
Small Airway and Alveolar Epithelial Progenitor Cell Enrichment: Distal Lung Tissue Preparation
2:16
Distal Lung Tissue Enzyme Digestion
3:03
Distal Lung Tissue Single Cell Isolation
4:34
Distal Lung Cell Surface Staining for Fluorescence Associated Cell Sorting (FACS)
5:45
Epithelial Progenitor Cell Enrichment from Tracheo-Bronchial Airways: Proximal Tissue Preparation
7:47
Proximal Lung Tissue Enzyme Digestion
8:51
Results: Representative Human Lung Epithelial Progenitor Cell Enrichment and Organoid Culture
10:48
Conclusion
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