Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii
13.0K Views
•
10:24 min
•
April 10th, 2020
DOI :
April 10th, 2020
•Chapters in this video
0:04
Introduction
0:55
Preparation of Bacteria for Membrane Extraction
2:18
Dissociation of the Outer Membrane and Plasmolysis
3:56
Pressurized Homogenization and Lysis
4:35
Total Membrane Fractionation
5:34
Separating the Dual Membranes
8:00
Results: Purity and Quality of Inner and Outer Membrane Fractions
9:36
Conclusion
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