Establishment and Analysis of Three-Dimensional (3D) Organoids Derived from Patient Prostate Cancer Bone Metastasis Specimens and their Xenografts
9.2K Views
•
07:21 min
•
February 3rd, 2020
DOI :
February 3rd, 2020
•Sanghee Lee1,2, Danielle N. Burner1,2, Theresa R. Mendoza1,2, Michelle T. Muldong1,2, Catalina Arreola1,2, Christina N. Wu2,3, Nicholas A. Cacalano4, Anna A. Kulidjian2,5, Christopher J. Kane1,2, Christina A. M. Jamieson1,2
1Department of Urology, University of California, San Diego, 2Moores Cancer Center, University of California, San Diego, 3Department of Medicine, University of California, San Diego, 4Department of Radiation Oncology, University of California, Los Angeles, 5Department of Orthopedic Surgery, University of California, San Diego
Chapters in this video
0:05
Introduction
1:24
Forming an Attached Round Dome
2:23
Forming Floating Beads
3:19
Organoid Processing for Histology
5:24
Results: 3D Organoids from Patient-derived Xenograft and Primary Tumor Tissue
6:17
Conclusion
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