Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
6.6K Views
•
08:34 min
•
January 8th, 2020
DOI :
January 8th, 2020
•Chapters in this video
0:05
Title
1:24
Detection of Double-crossover Recombinants of P. aeruginosa
2:24
Confirmation of Gene Deletion with Colony PCR
3:07
Visualization of Bioluminescence-labeled Bacteria in Mice
5:42
Inoculation of Animals with Bacterial Strains
6:28
Results: Evaluation of Attenuated P. aeruginosa Strain
7:24
Conclusion
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