Targeted Antibody Blocking by a Dual-Functional Conjugate of Antigenic Peptide and Fc-III Mimetics (DCAF)
6.7K Views
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09:39 min
•
September 17th, 2019
DOI :
September 17th, 2019
•1Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 2Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, 3MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University
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Chapters in this video
0:05
Title
0:57
Chemical Synthesis of the Hydrazine Derivative of a Fc-III Peptide
2:31
Cleavage of the SUMO Tag
4:09
Assembling of DCAF1 by Native Chemical Ligation
7:06
Mass Spectrometry and ELISA Assay
8:19
Results: Native Chemical Ligation and ELISA Assay
9:03
Conclusion
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