Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
13.5K Views
•
07:18 min
•
July 26th, 2019
DOI :
July 26th, 2019
•Chapters in this video
0:04
Title
0:59
Biosensor Hydration and Assay Set-Up
2:51
Loading of Ligand onto Biosensor and Washing Away Additional Ligand
3:43
Association of Analyte to Ligand and Dissociation of Analyte from Ligand
5:41
Results: A 28 Amino Acid Middle Region of GrgA Binds σ28 In Vitro
6:45
Conclusion
Related Videos
10:44
Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
30.4K Views
08:53
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
8.8K Views
13:57
Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
29.0K Views
09:18
Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
9.8K Views
08:29
Preparation of Keratin Hydrolysate from Chicken Feathers and Its Application in Cosmetics
19.3K Views
10:41
Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
8.2K Views
09:30
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
9.3K Views
08:46
Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification
5.8K Views
08:07
Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
1.1K Views
02:09
Biolayer Interferometry Technology to Detect Interactions between Ligand and Analyte
236 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved