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Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

30.5K Views

10:44 min

October 21st, 2016

DOI :

10.3791/54640-v

October 21st, 2016


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Fluorescence Anisotropy

Chapters in this video

0:05

Title

0:36

SBDS-FlAsH Tag Protein Expression and Purification

4:10

Elongation Factor Like-1 (EFL1) Protein Expression and Purification

7:07

Labeling of SBDS-FlAsH with the FlAsH Fluorescent Dye 4',5'-Bis(1,3,2 dithioarsolan-2-yl) Fluorescein and Fluorescence Anisotropy Experiments

8:50

Results: Evaluation of Protein-protein Interactions Using Fluorescence Anisotropy

9:55

Conclusion

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