JoVE Logo

Sign In

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

9.0K Views

11:39 min

June 9th, 2019

DOI :

10.3791/59551-v

June 9th, 2019


Explore More Videos

Aqueous Metabolites

Chapters in this video

0:04

Title

1:18

Cell Culture on Day 1

4:37

Preparation of Reagents and Pre-washing Centrifugal Filter Units

6:17

Cell Culture on Day 2

7:24

Extraction of Metabolites from Cultured Cells and Ultrafiltration of Cell Extracts

8:55

Results: Diamide Treatment

10:42

Conclusion

Related Videos

article

11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

22.4K Views

article

07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

11.6K Views

article

07:41

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

8.3K Views

article

11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

34.9K Views

article

12:16

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos

19.9K Views

article

11:25

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

33.0K Views

article

08:06

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

9.2K Views

article

07:25

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

4.3K Views

article

02:55

Arteriovenous Metabolomics to Measure In Vivo Metabolite Exchange in Brown Adipose Tissue

1.3K Views

article

14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

4.7K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved