Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
10.0K Views
•
09:37 min
•
May 2nd, 2019
DOI :
May 2nd, 2019
•Chapters in this video
0:04
Title
0:34
Harvesting Cells After Blocking Free Cysteine Residues Using Iodoacetamide
2:02
Extraction of Protein and Sample Preparation
3:13
In Vivo Formaldehyde Cross-linking of Endogenous Proteins in U-2 OS Cells
4:30
Fractionation of Nuclei
6:11
Extraction of Protein and Sample Preparation
6:51
SDS-PAGE Analysis
7:53
Results: Analyzing Multimeric Complexes Using a Two-step Method of Non-reducing SDS-PAGE Analysis and Formaldehyde Cross-linking
9:04
Conclusion
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