JoVE Logo

Sign In

Establishment and Characterization of Three Afatinib-resistant Lung Adenocarcinoma PC-9 Cell Lines Developed with Increasing Doses of Afatinib

7.9K Views

09:38 min

June 26th, 2019

DOI :

10.3791/59473-v

June 26th, 2019


Explore More Videos

Afatinib

Chapters in this video

0:04

Title

0:38

Initial Afatinib PC-9 Cell Exposure Determination by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) Assay

1:46

Continuous Afatinib Exposure

3:01

PC-9 Cell Growth Curve Determination

3:46

Epidermal Growth Factor Receptor (EGFR) Genomic DNA Alteration Evaluation

4:53

Epidermal Growth Factor Receptor (EGFR) Protein Alteration Evaluation

7:39

Results: Afatinib-resistant Cell Characterization

8:59

Conclusion

Related Videos

article

09:38

Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure

8.7K Views

article

15:43

Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts

23.3K Views

article

05:54

Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening

8.5K Views

article

00:09

Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines

6.9K Views

article

08:02

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

5.4K Views

article

08:52

Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids

3.9K Views

article

13:34

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

10.1K Views

article

06:08

Establishment and Characterization of Patient-Derived Xenograft Models of Anaplastic Thyroid Carcinoma and Head and Neck Squamous Cell Carcinoma

1.6K Views

article

07:08

Reprogramming Pancreatic Ductal Adenocarcinoma to Pluripotency

695 Views

article

03:36

3D Co-culture of Lung Cancer Cells with CAFs: An In Vitro Model System to Study Tumor Progression

2.3K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved