Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors
11.2K Views
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07:33 min
•
May 1st, 2019
DOI :
May 1st, 2019
•Maria G. Garone1, Valeria de Turris2, Alessandro Soloperto2, Carlo Brighi2, Riccardo De Santis3, Francesca Pagani2,4, Silvia Di Angelantonio2,4, Alessandro Rosa1,2
1Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Italy, 2Center for Life Nano Science, Istituto Italiano di Tecnologia, Italy, 3Laboratory of Stem Cell biology and Molecular Embryology, The Rockefeller University, USA, 4Department of Physiology and Pharmacology, Sapienza University of Rome, Italy
Chapters in this video
0:04
Title
0:54
Ngn2, Isl1 and Lhx3 (NIL) and Ngn2, Isl1 and Phox2a (NIP) Induced Pluripotent Stem Cell (iPSC) Line Generation
2:19
Antibiotic Selection
3:18
Motor Neuron Differentiation
5:12
Results: Representative Human iPSC Motor Neuron (MN) Differentiation Analysis
7:01
Conclusion
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