Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
14.8K Views
•
11:48 min
•
April 4th, 2019
DOI :
April 4th, 2019
•Camille Bouillier1, Vincent Rincheval1, Delphine Sitterlin1, Sabine Blouquit-Laye1, Aurore Desquesnes1, Jean-François Eléouët2, Elyanne Gault1,3, Marie-Anne Rameix-Welti1,3
1UMR 1173 Institut national de la santé et de la recherche médicale (INSERM), Université de Versailles St. Quentin, 2UR892 Institut national de la recherche agronomique (INRA), Unité de virologie et immunologie moléculaires, 3Assistance Publique–Hôpitaux de Paris (AP-HP), Hôpital Ambroise Paré, Laboratoire de Microbiologie
Chapters in this video
0:04
Title
0:40
Rescue and First Passage of Recombinant Virus
4:17
Amplification of the Rescued Viruses
6:30
Plaque Titration Assay
9:41
Results: An Optimized Titration Assay of Human RSV
11:22
Conclusion
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