Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation
7.4K Views
•
12:49 min
•
March 21st, 2020
DOI :
March 21st, 2020
•Ying Fan*1, Francesca Tonelli*1, Shalini Padmanabhan2, Marco A.S. Baptista2, Lindsey Riley2, Danielle Smith3, Connie Marras4, Andrew Howden5, Dario R. Alessi1, Esther Sammler1,6
1MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, 2The Michael J. Fox Foundation for Parkinson's Research, 3Department of Medical and Molecular Genetics, Indiana University School of Medicine, 4Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson's Disease, Toronto Western Hospital, University of Toronto, 5Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, 6Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School, University of Dundee
Chapters in this video
0:01
Introduction
0:48
Neutrophil Isolation from Human Peripheral Blood
7:18
LRRK2 Kinase Inhibitor Treatment
10:33
Results
12:14
Conclusion
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